Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (AB247806)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/100 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (AB247806)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/150 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

• IP

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Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (AB247806)

Purified ab109245 at 1/70 dilution (2 μg) immunoprecipitating SOCS2 in K-562 whole cell lysate.
Lane 1 (input) : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg
Lane 2 (+) : ab109245 + K-562 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109245 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 22 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

All lanes:

Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (<a href='/en-us/products/primary-antibodies/socs2-antibody-epr25882-ab109245'>ab109245</a>)

Predicted band size: 22 kDa

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• WB

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Western blot - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (AB247806)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

All lanes:

Western blot - Anti-SOCS2 antibody [EPR2588(2)] (<a href='/en-us/products/primary-antibodies/socs2-antibody-epr25882-ab109245'>ab109245</a>) at 1/2000 dilution

Lane 1:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

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