Rabbit Polyclonal SOCS3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 134 publications.
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- Molecular neurobiology 61:3105-31202023Beneficial Effect of Fingolimod in a Lafora Disease Mouse Model by Preventing Reactive Astrogliosis-Derived Neuroinflammation and Brain Infiltration of T-lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Rubio,Ángela Campos-Rodríguez,Pascual SanzPubMed 37971656
- World journal of gastroenterology 29:5226-52392023Curcumin alleviated dextran sulfate sodium-induced colitis by recovering memory Th/Tfh subset balance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Xin Zheng,Kai-En Guo,Jia-Qi Huang,Miao-Hua Liu,Bai-Ling Deng,Duan-Yong Liu,Bu-Gao Zhou,Wen Zhou,You-Bao Zhong,Hai-Mei ZhaoPubMed 37901446
- Journal of experimental & clinical cancer research : CR 42:1402023Kremen2 drives the progression of non-small cell lung cancer by preventing SOCS3-mediated degradation of EGFR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxiao Sun,Yu Gao,Mingxin Dong,Jiuzhen Li,Xin Li,Ningning He,Huijuan Song,Manman Zhang,Kaihua Ji,Jinhan Wang,Yeqing Gu,Yan Wang,Liqing Du,Yang Liu,Qin Wang,Hezheng Zhai,Daqiang Sun,Qiang Liu,Chang XuPubMed 37270563
- Frontiers in immunology 14:10885422023SOCS3 as a potential driver of lung metastasis in colon cancer patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuejie Li,Zuyi Yang,Bi Chen,Lei Gu,Guoyan Tian,Xinbing SuiPubMed 37025997
- PLoS pathogens 19:e10111662023Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXian-Zhang Wang,Le Wen,Yue-Peng Zhou,Sheng-Nan Huang,Bo Yang,Shuang Cheng,Wen-Bo Zeng,Meng-Jie Mei,Jin-Yan Sun,Xuan Jiang,Han Cheng,Min-Hua LuoPubMed 36753521
- Cell death & disease 14:552023PTK6 inhibits autophagy to promote uveal melanoma tumorigenesis by binding to SOCS3 and regulating mTOR phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Liu,Xueting Yao,Chaoyang Zhang,Yufen Liu,Li Wei,Qinying Huang,Mengting Wang,Yanchen Zhang,Danning Hu,Wencan WuPubMed 36690663
- International journal of molecular sciences 24:2022TSLP as a Potential Therapy in the Treatment of CRLF2 B Cell Acute Lymphoblastic Leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHossam R Alkashgari,Caleb Ruiz-Jimenez,Cornelia Stoian,Jacqueline S Coats,Ineavely Baez,Evgeny Chirshev,Shannalee R Martinez,Sinisa Dovat,Olivia L Francis-Boyle,Carlos A Casiano,Kimberly J PaynePubMed 36613920
- Brain, behavior, and immunity 107:76-862022Opening K channels induces inflammatory tolerance and prevents chronic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Qian,Yixin Fan,Lijuan Zong,Chen Miao,Lu-Lu Ji,Li Wan,Rumeng Jia,Xinmiao Qin,Yu Wang,Qi Wu,Xue-You Tao,Lanxiang Hao,Liang Hu,Wen-Tao LiuPubMed 36198341
- Folia histochemica et cytobiologica 60:226-2362022Activation of the JAK1/STAT1 signaling pathway is associated with peroxiredoxin 6 expression levels in human epididymis epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Shi,Xiaoyu Liu,Yanwei Wang,Haiyan Wang,Bochen Pan,Jianyuan LiPubMed 35929062
- Journal of inflammation research 15:3305-33212022Identification of Immune-Related Genes in Patients with Acute Myocardial Infarction Using Machine Learning Methods.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Zhu,Ting Yin,Ting Zhang,Qingqing Zhu,Xinyi Lu,Luyang Wang,Shengen Liao,Wenming Yao,Yanli Zhou,Haifeng Zhang,Xinli LiPubMed 35692951
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820).
Alternative names
Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3, CIS-3, SSI-3, SSI3, CIS3, SOCS3
Recommended products
Rabbit Polyclonal SOCS3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 134 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-SOCS3 antibody (ab16030)
The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3.
All lanes: Western blot - Anti-SOCS3 antibody (ab16030) at 1 µg/mL
Lanes 1 and 5: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 2 and 6: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lanes 3 and 7: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Lane 8: MOLT-4 (Human) Whole Cell Lysate (ab52252) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody (ab16030)
ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
This image is courtesy of an anonymous customer reviewWestern blot - Anti-SOCS3 antibody (ab16030)
All lanes: Western blot - Anti-SOCS3 antibody (ab16030) at 1 µg/mL
Lane 1: Untreated Human peripheral blood mononuclear cell lysate at 15 µg
Lane 2: Human peripheral blood mononuclear cell lysate (treated with aCD3/28) at 15 µg
Lane 3: Human peripheral blood mononuclear cell lysate (treated with IFNγ) at 15 µg
Lane 4: SOCS3 protein at 15 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 15min
Western blot - Anti-SOCS3 antibody (ab16030)
Blocking and dilution buffer: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as loading control at 1/1000000 dilution.
Lanes 1 - 3: Western blot - Anti-SOCS3 antibody [EPR24090-74] (Anti-SOCS3 antibody [EPR24090-74] ab280884) at 1/1000 dilution
Lanes 4 - 6: Western blot - Anti-SOCS3 antibody (ab16030) at 1/1000 dilution
Lanes 1 and 4: KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate at 20 µg
Lanes 2 and 5: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lanes 3 and 6: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa, 26 kDa
Exposure time: 180s
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