Anti-SOCS3 antibody

• WB

Supplier Data

Western blot - Anti-SOCS3 antibody (AB16030)

Blocking and dilution buffer : 5% NFDM/TBST.

ab181602 was used as loading control at 1/1000000 dilution.

Lanes 1 - 3:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (<a href='/en-us/products/primary-antibodies/socs3-antibody-epr24090-74-ab280884'>ab280884</a>) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-SOCS3 antibody (ab16030) at 1/1000 dilution

Lanes 1 and 4:

KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate at 20 µg

Lanes 2 and 5:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lanes 3 and 6:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa,26 kDa

false

Exposure time: 180s

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody (AB16030)

ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• WB

AbReview33034****

Western blot - Anti-SOCS3 antibody (AB16030)

All lanes:

Western blot - Anti-SOCS3 antibody (ab16030) at 1 µg/mL

Lane 1:

Untreated Human peripheral blood mononuclear cell lysate at 15 µg

Lane 2:

Human peripheral blood mononuclear cell lysate (treated with aCD3/28) at 15 µg

Lane 3:

Human peripheral blood mononuclear cell lysate (treated with IFN&gamma;) at 15 µg

Lane 4:

SOCS3 protein at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

true

Exposure time: 15min

This image is courtesy of an anonymous Abreview

• WB

Lab

Western blot - Anti-SOCS3 antibody (AB16030)

The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3.

All lanes:

Western blot - Anti-SOCS3 antibody (ab16030) at 1 µg/mL

Lanes 1 and 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lanes 2 and 6:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lanes 3 and 7:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 8:

MOLT-4 (Human) Whole Cell Lysate (ab52252) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 24 kDa

Observed band size: 30 kDa

false