Anti-SOCS3 antibody [EPR24090-74]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human skin. (PMID : 22739986) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stain Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in human colon. (PMID : 28603075) The section was incubated with ab280884 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta. (PMID : 29201185 ) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

SOCS3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2 : ab280884 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG1 (ab18443) instead of ab280884 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 58 seconds.

All lanes:

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (ab280884)

Predicted band size: 24 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in rat colon. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

SOCS3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug

Lane 2 : ab280884 IP in RAW 264.7 treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab280884 in treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (ab280884)

Predicted band size: 24 kDa

false

• WB

Lab

Western blot - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17114447, 32306216, 31238931）
SOCS3 exists in two isoforms of different length that are encoded by one gene
Negative control : skin (PMID : 22739986)

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Human skin tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse placenta tissue lysate at 20 µg

Lane 6:

Mouse skin tissue lysate at 20 µg

Lane 7:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa,26 kDa

false

• WB

Lab

Western blot - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17114447, 32306216, 31238931）

Exposure time : 26 seconds

All lanes:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution

Lane 1:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

RAW264.7 treated with 100ng/ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-SOCS3 antibody [EPR24090-74] (AB280884)

Blocking and dilution buffer : 5% NFDM/TBST.

ab181602 was used as loading control at 1/1000000 dilution.

Lanes 1 - 3:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-SOCS3 antibody (<a href='/en-us/products/primary-antibodies/socs3-antibody-ab16030'>ab16030</a>) at 1/1000 dilution

Lanes 1 and 4:

KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate at 20 µg

Lanes 2 and 5:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lanes 3 and 6:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa,26 kDa

false

Exposure time: 180s