Name: Anti-SOCS3 antibody [EPR24090-74]
SKU: ab280884
Rating: 5 (1 reviews)

Anti-SOCS3 antibody [EPR24090-74] (ab280884) is a rabbit monoclonal antibody that is used to detect SOCS3 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Images

Western blot - Anti-SOCS3 antibody [EPR24090-74] (AB280884), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	Flow Cyt	WB	IHC-P
Human	Not recommended	Tested	Not recommended	Tested	Tested
Mouse	Not recommended	Tested	Not recommended	Tested	Tested
Rat	Not recommended	Expected	Not recommended	Not recommended	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Target data

See full target information SOCS3

Function

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820).

Alternative names

CIS3, SSI3, SOCS3, Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3, CIS-3, SSI-3

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24090-74
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-SOCS3 antibody [EPR24090-74] (ab280884) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of SOCS3?

Anti-SOCS3 [EPR24090-74] (ab280884) specifically detects a band for SOCS3 (UniProt: O14543) at a molecular weight of 24kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR24090-74] also available for your convenience:
ab280884, Carrier free - ab280889

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17114447, 32306216, 31238931）
Exposure time: 26 seconds
All lanes: Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution
Lane 1: Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 2: RAW264.7 treated with 100ng/ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysates at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa
Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17114447, 32306216, 31238931）
SOCS3 exists in two isoforms of different length that are encoded by one gene
Negative control: skin (PMID: 22739986)
Exposure time: 3 minutes
All lanes: Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg
Lane 2: Human placenta tissue lysate at 20 µg
Lane 3: Human skin tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Mouse placenta tissue lysate at 20 µg
Lane 6: Mouse skin tissue lysate at 20 µg
Lane 7: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa, 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the stromal cells in human colon. (PMID: 28603075) The section was incubated with ab280884 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human placenta. (PMID: 29201185 ) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
SOCS3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab280884 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG1 (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of ab280884 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 58 seconds.
All lanes: Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse spleen. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
SOCS3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug
Lane 2: ab280884 IP in RAW 264.7 treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab280884 in treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the stromal cells in rat colon. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human skin. (PMID: 22739986) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stain Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884)
Blocking and dilution buffer: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as loading control at 1/1000000 dilution.
Lanes 1 - 3: Western blot - Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution
Lanes 4 - 6: Western blot - Anti-SOCS3 antibody (Anti-SOCS3 antibody ab16030) at 1/1000 dilution
Lanes 1 and 4: KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate at 20 µg
Lanes 2 and 5: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lanes 3 and 6: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa, 26 kDa
Exposure time: 180s