AB280889

Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SOCS3 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

CIS3, SSI3, SOCS3, Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3, CIS-3, SSI-3

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human skin. (PMID : 22739986) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stain Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in human colon. (PMID : 28603075) The section was incubated with ab280884 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta. (PMID : 29201185 ) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

SOCS3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2 : ab280884 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG1 (ab18443) instead of ab280884 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 58 seconds

All lanes:

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (<a href='/en-us/products/primary-antibodies/socs3-antibody-epr24090-74-ab280884'>ab280884</a>)

Predicted band size: 24 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in rat colon. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

SOCS3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug

Lane 2 : ab280884 IP in RAW 264.7 treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab280884 in treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] (<a href='/en-us/products/primary-antibodies/socs3-antibody-epr24090-74-ab280884'>ab280884</a>)

Predicted band size: 24 kDa

false

Lab

Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17114447, 32306216, 31238931）
SOCS3 exists in two isoforms of different length that are encoded by one gene
Negative control : skin (PMID : 22739986)

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (<a href='/en-us/products/primary-antibodies/socs3-antibody-epr24090-74-ab280884'>ab280884</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Human skin tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse placenta tissue lysate at 20 µg

Lane 6:

Mouse skin tissue lysate at 20 µg

Lane 7:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa,26 kDa

false

Lab

Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17114447, 32306216, 31238931）

Exposure time : 26 seconds

All lanes:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (<a href='/en-us/products/primary-antibodies/socs3-antibody-epr24090-74-ab280884'>ab280884</a>) at 1/1000 dilution

Lane 1:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

RAW264.7 treated with 100ng/ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Supplier Data

Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Blocking and dilution buffer : 5% NFDM/TBST.

ab181602 was used as loading control at 1/1000000 dilution.

This data was developed using ab280884, the same antibody clone in a different buffer formulation.

Lanes 1 - 3:

Western blot - Anti-SOCS3 antibody [EPR24090-74] (<a href='/en-us/products/primary-antibodies/socs3-antibody-epr24090-74-ab280884'>ab280884</a>) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-SOCS3 antibody (<a href='/en-us/products/primary-antibodies/socs3-antibody-ab16030'>ab16030</a>) at 1/1000 dilution

Lanes 1 and 4:

KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate at 20 µg

Lanes 2 and 5:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lanes 3 and 6:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa,26 kDa

false

Exposure time: 180s

Unconjugated

Anti-SOCS3 antibody [EPR24090-74]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24090-74

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab280889 is the carrier-free version of ab280884.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 15601820).

See full target information SOCS3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:78 PubMed38844873

2024

Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Liu,Mengbi Zhang,Lu Zeng,Yanhong Lai,Songzhao Wu,Xiaoyan Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (AB280889)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (1)

Product promise