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AB236519

Anti-SOCS3 antibody [OTI3D3]

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(5 Publications)

Mouse Monoclonal SOCS3 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SOCS3.

View Alternative Names

CIS3, SSI3, SOCS3, Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3, CIS-3, SSI-3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (AB236519)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (AB236519)

Paraffin-embedded human thyroid carcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (AB236519)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (AB236519)

Paraffin-embedded human colon adenocarcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-SOCS3 antibody [OTI3D3] (AB236519)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SOCS3 antibody [OTI3D3] (AB236519)

Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

Flow Cytometry (Intracellular) - Anti-SOCS3 antibody [OTI3D3] (AB236519)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SOCS3 antibody [OTI3D3] (AB236519)

Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] (AB236519)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] (AB236519)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA stained for SOCS3 (green) using ab236519 at 1/100 dilution in ICC/IF.

Western blot - Anti-SOCS3 antibody [OTI3D3] (AB236519)
  • WB

Supplier Data

Western blot - Anti-SOCS3 antibody [OTI3D3] (AB236519)

All lanes:

Western blot - Anti-SOCS3 antibody [OTI3D3] (ab236519) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate at 5 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3D3

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SOCS3.

O14543

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Perform heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Clone OTI3D3 (formerly 3D3).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 15601820).
See full target information SOCS3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e36340 PubMed39253270

2024

M1 macrophages as promising agents for cell therapy of endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Artemova,Polina Vishnyakova,Andrey Elchaninov,Elena Gantsova,Gennady Sukhikh,Timur Fatkhudinov

Heliyon 10:e33835 PubMed39050450

2024

OIP5-AS1 enhances the malignant characteristics and resistance to chemotherapy of pancreatic cancer cells by targeting miR-30d-5p/MARCH8.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Ying,Kening Li,Chao Chen,Ying Wang,Qing Zhao,Yaohui Wang,Lichao Xu,Haozhe Huang,Ge Song,Wentao Li,Xinhong He

Journal of gastrointestinal oncology 14:1669-1693 PubMed37720449

2023

Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Ruijiang Lin,Minjie Ma,Biao Han,Ya Zheng,Yuping Wang,Yongning Zhou

Journal of experimental & clinical cancer research : CR 42:140 PubMed37270563

2023

Kremen2 drives the progression of non-small cell lung cancer by preventing SOCS3-mediated degradation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiao Sun,Yu Gao,Mingxin Dong,Jiuzhen Li,Xin Li,Ningning He,Huijuan Song,Manman Zhang,Kaihua Ji,Jinhan Wang,Yeqing Gu,Yan Wang,Liqing Du,Yang Liu,Qin Wang,Hezheng Zhai,Daqiang Sun,Qiang Liu,Chang Xu

EBioMedicine 80:104019 PubMed35490460

2022

ALKBH5 regulates STAT3 activity to affect the proliferation and tumorigenicity of osteosarcoma via an m6A-YTHDF2-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Zechuan Yang,Zhuo Cai,Caihong Yang,Zhengqiang Luo,Xing Bao
View all publications
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Product promise

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