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AB224589

Anti-SOCS7 antibody

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(4 Publications)

Rabbit Polyclonal SOCS7 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOCS7 aa 1-200.

View Alternative Names

NAP4, SOCS6, SOCS7, Suppressor of cytokine signaling 7, SOCS-7, Nck-associated protein 4, NAP-4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SOCS7 antibody (AB224589)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOCS7 antibody (AB224589)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SOCS7 (green) using ab224589 at 1/100 dilution in ICC/IF.

Western blot - Anti-SOCS7 antibody (AB224589)
  • WB

Supplier Data

Western blot - Anti-SOCS7 antibody (AB224589)

All lanes:

Western blot - Anti-SOCS7 antibody (ab224589) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Lane 5:

Rat heart tissue lysate

Lane 6:

Rat liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SOCS7 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O14512

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/4998", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DAB1 and IRS1 (PubMed : 16127460). Specifically recognizes and binds phosphorylated proteins via its SH2 domain, promoting their ubiquitination (By similarity). The ECS(SOCS7) complex acts as a key regulator of reelin signaling by mediating ubiquitination and degradation of phosphorylated DAB1 in the cortical plate of the developing cerebral cortex, thereby regulating neuron positioning during cortex development (By similarity). Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation (PubMed : 16127460). Also inhibits prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA (PubMed : 15677474).
See full target information SOCS7
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 41:185 PubMed35624501

2022

SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhua Du,Xiang Xu,Siang Lv,Han Liu,Hong Sun,Jun Wu

PeerJ 9:e11374 PubMed34040893

2021

DZNep, an inhibitor of the histone methyltransferase EZH2, suppresses hepatic fibrosis through regulating miR-199a-5p/SOCS7 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rongrong Ding,Jianming Zheng,Ning Li,Qi Cheng,Mengqi Zhu,Yanbing Wang,Xinlan Zhou,Zhanqing Zhang,Guangfeng Shi

Frontiers in oncology 11:668743 PubMed33968776

2021

Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Hongyan Li,Lei Wu,Xueli Jiao,Zihui Jin,Yujie Zhu,Ziling Fang,Xiaodong Zhang,Haishan Huang,Lingling Zhao

Oncology letters 16:65-72 PubMed29928388

2018

miR-885-5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling.

Applications

WB

Species

Human

Meng Su,Baoli Qin,Fang Liu,Yuze Chen,Rui Zhang
View all publications
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