Rabbit Monoclonal Superoxide Dismutase 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human, Cat, Dog samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Cow SOD1.
Images
![Western blot - Anti-SOD1 antibody [HL1653] (AB308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--western-blot-img407197.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD1 antibody [HL1653] (AB308181)")
![Western blot - Anti-SOD1 antibody [HL1653] (AB308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--western-blot-img407200.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD1 antibody [HL1653] (AB308181)")
![Western blot - Anti-SOD1 antibody [HL1653] (AB308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--western-blot-img407199.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD1 antibody [HL1653] (AB308181)")
![Immunohistochemistry paraffin embedded sections - Anti-SOD1 antibody [HL1653] (AB308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--immunohistochemistry-paraffin-embedded-sections-img407198.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-SOD1 antibody [HL1653] (AB308181)")
Publications
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- International journal of molecular sciences 24:2023Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia de Las Heras,Adrián Galiana,Sandra Ballesteros,José Carlos Quintela,Ileana Bonilauri,Vicente Lahera,Beatriz Martín-FernándezPubMed 37175685
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Cow SOD1. The exact immunogen used to generate this antibody is proprietary information. Database link P00442
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cat | Tested | Expected |
| Dog | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Cat | Dilution info 1/1000 | Notes - |
Species Dog | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Alternative names
Superoxide dismutase [Cu-Zn], SOD1
Recommended products
Rabbit Monoclonal Superoxide Dismutase 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human, Cat, Dog samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Cow SOD1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Cow SOD1. The exact immunogen used to generate this antibody is proprietary information. Database link P00442
- Carrier free
- Yes
- Clone number
- HL1653
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-SOD1 antibody [HL1653] (ab308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--western-blot-img407197.jpg "Western blot - Anti-SOD1 antibody [HL1653] (ab308181)")
Western blot - Anti-SOD1 antibody [HL1653] (ab308181)
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1653] (ab308181) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SOD1 antibody [HL1653] (ab308181) at 1/1000 dilution
Lane 1: Mouse brain lysate at 50 µg
Lane 2: Rat brain lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 15.9 kDa
![Western blot - Anti-SOD1 antibody [HL1653] (ab308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--western-blot-img407200.jpg "Western blot - Anti-SOD1 antibody [HL1653] (ab308181)")
Western blot - Anti-SOD1 antibody [HL1653] (ab308181)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1653] (ab308181) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SOD1 antibody [HL1653] (ab308181) at 1/1000 dilution
Lane 1: MDCK cell lysate at 30 µg
Lane 2: PG-4 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 15.9 kDa
![Western blot - Anti-SOD1 antibody [HL1653] (ab308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--western-blot-img407199.jpg "Western blot - Anti-SOD1 antibody [HL1653] (ab308181)")
Western blot - Anti-SOD1 antibody [HL1653] (ab308181)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1653] (ab308181) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SOD1 antibody [HL1653] (ab308181) at 1/1000 dilution
Lane 1: A431 cell lysate at 30 µg
Lane 2: HeLa cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 15.9 kDa
![Immunohistochemistry paraffin embedded sections - Anti-SOD1 antibody [HL1653] (ab308181), expandable thumbnail](https://content.abcam.com/products/images/sod1-antibody-hl1653-ab308181--immunohistochemistry-paraffin-embedded-sections-img407198.jpg "Immunohistochemistry paraffin embedded sections - Anti-SOD1 antibody [HL1653] (ab308181)")
Immunohistochemistry paraffin embedded sections - Anti-SOD1 antibody [HL1653] (ab308181)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling SOD1 protein with ab308181 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.
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