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AB317090

Anti-SOD2 antibody [HL1483]

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Rabbit Recombinant Monoclonal SOD2/MnSOD antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Zebrafish samples. Immunogen corresponding to Synthetic Peptide within Human Superoxide dismutase [Mn], mitochondrial conjugated to Keyhole Limpet Haemocyanin.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2 antibody [HL1483] (AB317090)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2 antibody [HL1483] (AB317090)

ab317090 detects SOD2 protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded human breast carcinoma.
SOD2 stained by ab317090 diluted at 1 : 100.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-SOD2 antibody [HL1483] (AB317090)
  • WB

Supplier Data

Western blot - Anti-SOD2 antibody [HL1483] (AB317090)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ab317090 diluted at 1 : 2000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SOD2 antibody [HL1483] (ab317090) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lanes 2 - 3:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SOD2 antibody [HL1483] (AB317090)
  • WB

Supplier Data

Western blot - Anti-SOD2 antibody [HL1483] (AB317090)

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ab317090 diluted at 1 : 1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SOD2 antibody [HL1483] (ab317090) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1483

Isotype

IgG

Carrier free

No

Reacts with

Human, Zebrafish

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Superoxide dismutase [Mn], mitochondrial conjugated to Keyhole Limpet Haemocyanin.

P04179

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
See full target information Superoxide dismutase [Mn], mitochondrial

Alternative Names

SOD2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com