AB13533

Anti-SOD2/MnSOD antibody

Name: Anti-SOD2/MnSOD antibody (ab13533) | Abcam
Brand: Abcam Limited
SKU: AB13533
Price: 505 USD
Availability: InStock
Rating: 5 (30 reviews)

(30 Reviews)

(383 Publications)

Anti-SOD2/MnSOD antibody (ab13533) is a rabbit polyclonal antibody detecting SOD2/MnSOD in Western Blot. Suitable for Mouse, Rat.

- Over 320 publications
- Trusted since 2004

View Alternative Names

SOD2

3 Images

Supplier Data

Western blot - Anti-SOD2/MnSOD antibody (AB13533)

Block : 5% Skim Milk powder in TBST.

Color Development : Chemiluminescent for HRP for 5 min at RT.

All lanes:

Western blot - Anti-SOD2/MnSOD antibody (ab13533) at 1/1000 dilution

Lane 1:

Mouse brain at 15 µg

Lane 2:

Rat brain at 15 µg

Lane 3:

Rat brain membrane at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP for 1 hr at RT with shaking at 1/5000 dilution

Predicted band size: 24 kDa

false

AbReview6714****

Western blot - Anti-SOD2/MnSOD antibody (AB13533)

All lanes:

Western blot - Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

Lane 1:

2ug mouse liver homogenate supernatant

Lane 2:

1ug mouse liver homogenate supernatant

Lane 3:

0.5ug mouse liver homogenate supernatant

Secondary

All lanes:

HRP conjugated donkey anti-rabbit antibody

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Exposure time: 10s

This image is courtesy of an Abreview submitted by Dr sandra sobocanec

AbReview46903****

Western blot - Anti-SOD2/MnSOD antibody (AB13533)

All lanes:

Western blot - Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

Lane 1:

mouse liver whole cell lysate

Lane 2:

mouse hepatocytes whole cell lysate

Secondary

All lanes:

Goat anti-rabbit HRP conjugate at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

true

Exposure time: 15s

Image courtesy of anonymous abreviewer

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Human Superoxide dismutase [Mn], mitochondrial. The exact immunogen used to generate this antibody is proprietary information.

P04179

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-SOD2/MnSOD antibody (ab13533) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Mouse, Rat samples.

What is the molecular weight of SOD2/MnSOD?
Anti-SOD2/MnSOD (ab13533) specifically detects a band for SOD2/MnSOD (UniProt: P04179) at a molecular weight of 26.6kDa.

Trusted by the scientific community
Anti-SOD2/MnSOD (ab13533) was first used in a scientific publication in 2004 and has been cited over 320 times in peer-reviewed journals.

Reviewed by scientists
Anti-SOD2/MnSOD (ab13533) has over 30 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This antibody was immunoaffinity purified.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

See full target information Superoxide dismutase [Mn], mitochondrial

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Publications (383)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:643 PubMed41074154

2025

Microneedles combining delivery of hUMSC-derived exosomes and EGCG mitigate UV-induced skin damage.

Applications

Unspecified application

Species

Unspecified reactive species

Chenhui He,Zeqian Wang,Zhaoting Jiang,Yuqi Zhou,Xiaoqi Chen,Jia Zhang,Bo Wang,Qi Wang,Tong Wu,Chunying Li,Zhe Jian

Redox biology 86:103814 PubMed40789258

2025

Inhibition of eIF5A hypusination enhances antioxidant defense to prevent kidney Ischemia/Reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Cougnon,Sébastien Giraud,Maria Materozzi,Elisa Allart,Isabelle Rubera,Claire Mackowiak,Gisèle Jarretou,Nadège Boildieu,Virginie Ameteau,Estelle Lemarié,Hajar Ouahmi,Nicolas Melis,Mallorie Poet,Christophe Duranton,Luc Pellerin,Laurent Counillon,Marina Shkreli,Michel Tauc,Thierry Hauet,Didier F Pisani

JBMR plus 9:ziaf092 PubMed40584154

2025

Sirtuin-3 promotes osteoclast maturation and bone loss by regulating mitochondrial ROS production during ionizing radiation exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Gareeballah Osman Adam,Kimberly K Richardson,Ankita Chalke,Qiang Fu,Jeff D Thostenson,Hutomo Tanoto,Yuxiao Zhou,Nukhet Aykin-Burns,Ha-Neui Kim

The Journal of biological chemistry 301:108451 PubMed40147772

2025

Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron Naatz,Kelsey S Bohl,Rachel A Jones Lipinski,Joshua A Nord,Alyssa L Gehant,Polly A Hansen,Brian C Smith,John A Corbett

Medical gas research 15:383-390 PubMed39923134

2025

Variability in flow-induced vasodilation mechanisms in cerebral arteries: the impact of different hyperbaric oxygen protocols.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Drenjančević,Ivana Jukić,Vedran Đambić,Ana Stupin,Nataša Kozina,Anita Matić,Petar Šušnjara,Aleksandar Kibel,Darko Biljan,Zrinka Mihaljević

In vitro models 3:33-48 PubMed39872068

2025

Modelling neurodegeneration and inflammation in early diabetic retinopathy using 3D human retinal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Luisa de Lemos,Pedro Antas,Inês S Ferreira,Inês Paz Santos,Beatriz Felgueiras,Catarina M Gomes,Catarina Brito,Miguel C Seabra,Sandra Tenreiro

Redox report : communications in free radical research 30:2454892 PubMed39869517

2025

M6a demethylase FTO regulates the oxidative stress, mitochondrial biogenesis of cardiomyocytes and PGC-1a stability in myocardial ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Jiang,Xuehai Chen,Kezeng Gong,Zhe Xu,Lianglong Chen,Feilong Zhang

Journal of cellular and molecular medicine 28:e70192 PubMed39517099

2024

Hydrogen sulfide alleviates neural degeneration probably by reducing oxidative stress and aldose reductase expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Shen,Tingyu Hu,Xin Wang,Xiaoyan Zhang,Junxi Lu,Huijuan Lu,Yanyun Hu,Fang Liu

Frontiers in pharmacology 15:1427985 PubMed39411067

2024

Xiaoyin-anshen formula alleviates psoriasis complicated by sleep disturbances by regulating melatonin, antioxidant enzymes, and pro-inflammatory cytokines in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zebing Zhu,Qiang Yin,Xingwu Duan

Autophagy 21:407-423 PubMed39291751

2024

Serine ubiquitination of SQSTM1 regulates NFE2L2-dependent redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rukmini Mukherjee,Anshu Bhattacharya,João Mello-Vieira,Santosh Kumar Kuncha,Marina Hoffmann,Alexis Gonzalez,Rajeshwari Rathore,Attinder Chadha,Donghyuk Shin,Thomas Colby,Ivan Matic,Shaeri Mukherjee,Mohit Misra,Ivan Dikic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOD2/MnSOD antibody

Western blot - Anti-SOD2/MnSOD antibody (AB13533)

Western blot - Anti-SOD2/MnSOD antibody (AB13533)

Western blot - Anti-SOD2/MnSOD antibody (AB13533)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (383)

Product promise