AB13534

Anti-SOD2/MnSOD antibody

Name: Anti-SOD2/MnSOD antibody. Rabbit polyclonal (ab13534) | Abcam
Brand: Abcam Limited
SKU: AB13534
Price: 585 USD
Availability: InStock
Rating: 4 (14 reviews)

(14 Reviews)

(108 Publications)

Rabbit Polyclonal SOD2/MnSOD antibody. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 108 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Superoxide dismutase [Mn], mitochondrial.

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SOD2

5 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (AB13534)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification : 100x.

Left : DAPI (blue) nuclear stain. Middle : SOD2/MnSOD. Right : Merge.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

ICC/IF

AbReview10512****

Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (AB13534)

ab13534 staining differentiating (rat) PC12 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 2 μg/ml. An Alexa Fluor^® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary. Cells were counterstained with the nuclear TOPRO-3 stain.

This image is courtesy of an Abreview submitted by Dr Anne Chiaramello

IHC-P

AbReview35090****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.

This image is courtesy of an anonymous Abreview

Supplier Data

Western blot - Anti-SOD2/MnSOD antibody (AB13534)

All lanes:

Western blot - Anti-SOD2/MnSOD antibody (ab13534) at 1/1000 dilution

All lanes:

Rat Tissue lysates at 15 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG: HRP

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Rat Superoxide dismutase [Mn], mitochondrial. The exact immunogen used to generate this antibody is proprietary information.

P07895

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "10 µg/mL", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "10 µg/mL", "IP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This antibody was immunoaffinity purified.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

See full target information Superoxide dismutase [Mn], mitochondrial

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Publications (108)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45:2189-2202 PubMed40569616

2025

Assessing the effects of aging on the Koizumi's and Zea Longa's methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyeon Lim,Hongrae Kim,Sehyun Lim,Hyo-Eun Kim,So-Jung Moon,Hyung-Hwan Kim,Suin Cho

Journal of nanobiotechnology 23:439 PubMed40514650

2025

Nanoscale engineered exosomes for dual delivery of Sirtuin3 and insulin to ignite mitochondrial recovery in myocardial ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Yang,Xinyi Yun,Weihan Zheng,Huihui Zhang,Zi Yan,Youyu Chen,Wanting Xue,Siqi Mi,Ziyue Li,Hanxiao Sun,Guozhi Xiao,Zhenning Dai,Shiyu Li,Wenhua Huang

Frontiers in pharmacology 16:1538857 PubMed40176894

2025

Teriparatide mitigates oxidative stress following spinal cord injury and enhances neurological recovery via the Nrf2/HO-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Moliang,Ai Gangtong,Feng Yun,Luo Caiguang,Deng Liang,Guo Jia,Xiao Qiang

Frontiers in pharmacology 15:1427985 PubMed39411067

2024

Xiaoyin-anshen formula alleviates psoriasis complicated by sleep disturbances by regulating melatonin, antioxidant enzymes, and pro-inflammatory cytokines in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zebing Zhu,Qiang Yin,Xingwu Duan

BMC research notes 17:155 PubMed38840123

2024

Aspartame, as an artificial sweetener, does not affect renal function and antioxidative states in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Torigoe,Miki Torigoe,Satoru Oka,Yoko Obata,Hiroshi Mukae,Tomoya Nishino

Redox biology 71:103091 PubMed38412803

2024

SOD2 orchestrates redox homeostasis in intervertebral discs: A novel insight into oxidative stress-mediated degeneration and therapeutic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Shota Tamagawa,Daisuke Sakai,Hidetoshi Nojiri,Yoshihiko Nakamura,Takayuki Warita,Erika Matsushita,Jordy Schol,Hazuki Soma,Shota Ogasawara,Daiki Munesada,Masato Koike,Takahiko Shimizu,Masato Sato,Muneaki Ishijima,Masahiko Watanabe

The Journal of physiology 601:4699-4721 PubMed37815420

2023

Interleukin-1β triggers muscle-derived extracellular superoxide dismutase expression and protects muscles from doxorubicin-induced atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Mami Yamada,Mitsuharu Okutsu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23156 PubMed37624620

2023

Muscle p62 stimulates the expression of antioxidant proteins alleviating cancer cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Mami Yamada,Eiji Warabi,Hisashi Oishi,Vitor A Lira,Mitsuharu Okutsu

PloS one 18:e0286903 PubMed37310976

2023

The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ding,Sheng Cui,Song Yu Li,Lin Yan Cui,Qi Yan Nan,Xue Jing Lin,Mei Ying Xuan,Jian Jin,Shang Guo Piao,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Byung Ha Chung,Chul Woo Yang,Jing Hao Cui,Can Li

Nature 617:386-394 PubMed37100912

2023

A druggable copper-signalling pathway that drives inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphanie Solier,Sebastian Müller,Tatiana Cañeque,Antoine Versini,Arnaud Mansart,Fabien Sindikubwabo,Leeroy Baron,Laila Emam,Pierre Gestraud,G Dan Pantoș,Vincent Gandon,Christine Gaillet,Ting-Di Wu,Florent Dingli,Damarys Loew,Sylvain Baulande,Sylvère Durand,Valentin Sencio,Cyril Robil,François Trottein,David Péricat,Emmanuelle Näser,Céline Cougoule,Etienne Meunier,Anne-Laure Bègue,Hélène Salmon,Nicolas Manel,Alain Puisieux,Sarah Watson,Mark A Dawson,Nicolas Servant,Guido Kroemer,Djillali Annane,Raphaël Rodriguez

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SOD2/MnSOD antibody

Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (AB13534)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (AB13534)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

Western blot - Anti-SOD2/MnSOD antibody (AB13534)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (108)

Product promise