Rabbit Polyclonal SOD2/MnSOD antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 94 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Superoxide dismutase [Mn], mitochondrial.
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- The Journal of physiology 601:4699-47212023Interleukin-1β triggers muscle-derived extracellular superoxide dismutase expression and protects muscles from doxorubicin-induced atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMami Yamada,Mitsuharu OkutsuPubMed 37815420
- PloS one 18:e02869032023The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Ding,Sheng Cui,Song Yu Li,Lin Yan Cui,Qi Yan Nan,Xue Jing Lin,Mei Ying Xuan,Jian Jin,Shang Guo Piao,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Byung Ha Chung,Chul Woo Yang,Jing Hao Cui,Can LiPubMed 37310976
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1845-18572023Neuronal SIRT3 Deletion Predisposes to Female-Specific Alterations in Cellular Metabolism, Memory, and Network Excitability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer N Pearson-Smith,Ruth Fulton,Christopher Q Huynh,Anna G Figueroa,Gia B Huynh,Li-Ping Liang,Lindsey B Gano,Cole R Michel,Nichole Reisdorph,Richard Reisdorph,Kristofer S Fritz,Eric Verdin,Manisha PatelPubMed 36759193
- PloS one 17:e02741162022Inhibition of RIP1-RIP3-mediated necroptosis attenuates renal fibrosis via Wnt3α/β-catenin/GSK-3β signaling in unilateral ureteral obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShang Guo Piao,Jun Ding,Xue Jing Lin,Qi Yan Nan,Mei Ying Xuan,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Can LiPubMed 36223414
- Journal of neuroinflammation 19:1682022Targeted BRD4 protein degradation by dBET1 ameliorates acute ischemic brain injury and improves functional outcomes associated with reduced neuroinflammation and oxidative stress and preservation of blood-brain barrier integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Liu,Changjun Yang,Bianca P Lavayen,Ryland J Tishko,Jonathan Larochelle,Eduardo Candelario-JalilPubMed 35761277
- BioMed research international 2022:20640132022Kukoamine A Improves Pneumonia by Regulating miR-222-3p/Superoxide Dismutase 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiu-Xiu Liu,Ming-Jing Wang,Qian-Na Kan,Cui Li,Zhen Xiao,Yong-Hong Jiang,Wen Li,Xiao Li,Zhi-Yan JiangPubMed 35774277
- Food chemistry. Molecular sciences 4:1000692022Peptides GWN and GW protect kidney cells against Dasatinib induced mitochondrial injury in a SIRT1 dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhushwant S Bhullar,Fatemeh Ashkar,Jianping WuPubMed 35415678
- Nature communications 13:14262022Maintenance of mitochondrial integrity in midbrain dopaminergic neurons governed by a conserved developmental transcription factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederico Miozzo,Eva P Valencia-Alarcón,Luca Stickley,Michaëla Majcin Dorcikova,Francesco Petrelli,Damla Tas,Nicolas Loncle,Irina Nikonenko,Peter Bou Dib,Emi NagoshiPubMed 35301315
- Kidney & blood pressure research 47:277-2902022Effects of L-Carnitine Treatment on Kidney Mitochondria and Macrophages in Mice with Diabetic Nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeigo Ito,Masahiro Nakashima,Takuya Ishikiriyama,Hiroyuki Nakashima,Akira Yamagata,Toshihiko Imakiire,Manabu Kinoshita,Shuhji Seki,Hiroo Kumagai,Naoki OshimaPubMed 35104825
- Frontiers in pharmacology 12:7983812022Dapagliflozin Alleviates Renal Fibrosis by Inhibiting RIP1-RIP3-MLKL-Mediated Necroinflammation in Unilateral Ureteral Obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Ying Xuan,Shang Guo Piao,Jun Ding,Qi Yan Nan,Mei Hua Piao,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Can LiPubMed 35069210
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Superoxide dismutase [Mn], mitochondrial. The exact immunogen used to generate this antibody is proprietary information. Database link P07895
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Reactivity data
Select an application| IP | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
Species Rat | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Alternative names
SOD2
Recommended products
Rabbit Polyclonal SOD2/MnSOD antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 94 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Superoxide dismutase [Mn], mitochondrial.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Superoxide dismutase [Mn], mitochondrial. The exact immunogen used to generate this antibody is proprietary information. Database link P07895
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was immunoaffinity purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13534)
ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.
This image is courtesy of a customer review submitted by Dr Anne ChiaramelloImmunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13534)
ab13534 staining differentiating (rat) PC12 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 2 μg/ml. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary. Cells were counterstained with the nuclear TOPRO-3 stain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13534)
ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13534)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification: 100x.
Left: DAPI (blue) nuclear stain. Middle: SOD2/MnSOD. Right: Merge.
Immunoprecipitation - Anti-SOD2/MnSOD antibody (ab13534)
Lane 1: ab13534 + Mouse brain lysate (250μg)
Lane 2: ab13534 + Mouse brain lysate (250μg, ½ dilution)
Lane 3: Mouse brain lysate (15μg)
Lane 4: Rat brain lysate (15μg)ab13534 at 1:1000 immunoprecipitating SOD2 in mouse brain and rat brain lysate. For western blotting, donkey anti- rabbit IgG (HRP) was used as the secondary antibody.
All lanes: Immunoprecipitation - Anti-SOD2/MnSOD antibody (ab13534)
Predicted band size: 24 kDa
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