Rabbit Recombinant Monoclonal SOD2/MnSOD antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 8 publications.
Images
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (AB227846), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-bsa-and-azide-free-ab227846--western-blot-img141117.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (AB227846)")
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (AB227846), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-bsa-and-azide-free-ab227846--western-blot-img137056.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (AB227846)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (AB227846), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-bsa-and-azide-free-ab227846--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22715.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (AB227846)")
Publications
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- Scientific reports 7:79532017Inflammation-mediated SOD-2 upregulation contributes to epithelial-mesenchymal transition and migration of tumor cells in aflatoxin G-induced lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Yi et. al.PubMed 28801561
- Experimental and therapeutic medicine 13:2537-25432017Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Wang et. al.PubMed 28565876
- Oncotarget 8:22513-225232017AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema.Applications:WBReactive species:MouseXiao-Yu Cheng,Yang-Yang Li,Cheng Huang,Jun Li,Hong-Wei YaoPubMed 28186975
- PloS one 11:e01655802016Decreased Integrity, Content, and Increased Transcript Level of Mitochondrial DNA Are Associated with Keratoconus.
- Molecules (Basel, Switzerland) 21:2016Capsaicin Suppresses Cell Proliferation, Induces Cell Cycle Arrest and ROS Production in Bladder Cancer Cells through FOXO3a-Mediated Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiyu Qian et. al.PubMed 27775662
- Oxidative medicine and cellular longevity 2014:3205132014Saponins from Aralia taibaiensis attenuate D-galactose-induced aging in rats by activating FOXO3a and Nrf2 pathways.
- Virus research 179:187-2032013Hepatocyte pathway alterations in response to in vitro Crimean Congo hemorrhagic fever virus infection.Applications:Unspecified applicationReactive species:HumanChristophe Fraisier et. al.PubMed 24184319
- Respiratory research 12:1192011Increased oxidative stress and severe arterial remodeling induced by permanent high-flow challenge in experimental pulmonary hypertension.Applications:IHC-FrReactive species:RatPeter Dorfmüller,Marie-Camille Chaumais,Maria Giannakouli,Ingrid Durand-Gasselin,Nicolas Raymond,Elie Fadel,Olaf Mercier,Frédéric Charlotte,David Montani,Gérald Simonneau,Marc Humbert,Frédéric PerrosPubMed 21906276
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Expected | Expected |
| Rat | Not recommended | Not recommended | Not recommended | Expected | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Alternative names
SOD2
Recommended products
Rabbit Recombinant Monoclonal SOD2/MnSOD antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2560Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227846 is the carrier-free version of Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-bsa-and-azide-free-ab227846--western-blot-img141117.jpg "Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)")
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
This data was developed using the same antibody clone in a different buffer formulation (Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 observed at 22 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line Human SOD2 (MnSOD) knockout HEK-293T cell line ab255434 (knockout cell lysate Human SOD2 (MnSOD) knockout HEK-293T cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155) at 1/1000 dilution
Lane 1: SHSY5Y cell lysate at 20 µg
Lane 2: A549 cell lysate at 20 µg
Lane 2: Western blot - Human SOD2 (MnSOD) knockout HEK-293T cell line (Human SOD2 (MnSOD) knockout HEK-293T cell line ab255434)
Lane 3: Wild-type HEK-293T cell lysate at 20 µg
Lane 4: SOD2 knockout HEK-293T cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 29 kDa, 37 kDa
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-bsa-and-azide-free-ab227846--western-blot-img137056.jpg "Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)")
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
This WB data was generated using the same anti-SOD2 antibody clone, EPR2560Y, in a different buffer formulation (Cat# Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 observed at 29 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155) at 1/1000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: SOD2 knockout HAP1 cell lysate at 20 µg
Lane 3: Human heart tissue lysate at 20 µg
Lane 4: Rat heart tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 24 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-bsa-and-azide-free-ab227846--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22715.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
This IHC data was generated using the same anti-SOD2 antibody clone, EPR2560Y, in a different buffer formulation (cat# Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155).
Anti-SOD2/MnSOD antibody [EPR2560Y] ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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