AB74231

Anti-SOD2/MnSOD antibody [mAbcam 74231]

Name: Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) | Abcam
Brand: Abcam Limited
SKU: AB74231
Price: 535 USD
Availability: InStock
Rating: 3 (3 reviews)

KO Validated
What is this?

(3 Reviews)

(10 Publications)

Mouse Monoclonal SOD2/MnSOD antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Rat, Human samples. Cited in 10 publications.

View Alternative Names

SOD2

3 Images

Unknown

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)

The Superoxide dismutase 2 protein has a predicted molecular weight of 25-kDa. However, the sequence contains a 24 residue transit peptide at the N-terminus and, when cleaved, the protein has an expected molecular weight of approximately 23-kDa.

We recommend using 3% milk as the blocking agent in Western Blot.

All lanes:

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 5 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 3:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 4:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa

true

Exposure time: 5min

Lab

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)

Lane 1 : Wild-type HEK-293T cell lysate (40 ug) Lane 2 : Empty Lane Lane 3 : SOD2 knockout HEK-293T cell lysate (40 ug) Lanes 1 - 3 : Merged signal (red and green). Green - ab74231 observed at 25 kDa. Orange - loading control, ab181602, observed at 37 kDa. Gel type : MES Blocking buffer : 3% Milk ab74231 was shown to specifically react with SOD2. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab74231 and ab181602 GAPDH (loading control) were diluted 1 μg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 40 µg

Lane 3:

SOD2 knockout HEK-293T cell lysate at 40 µg

Secondary

Lanes 1 and 3:

Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed at 1/10000 dilution

Lanes 1 and 3:

Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Unknown

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)

All lanes:

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/mL

All lanes:

Recombinant Human SOD2/MnSOD protein (<a href='/en-us/products/unavailable/recombinant-human-sod2mnsod-protein-ab82656'>ab82656</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 24 kDa

true

Exposure time: 12min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 74231

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "We recommend using 3% milk as the blocking agent in Western Blot." }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "We recommend using 3% milk as the blocking agent in Western Blot." }, "Chimpanzee": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "We recommend using 3% milk as the blocking agent in Western Blot." }, "Zebrafish": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

This monoclonal antibody to SOD2 has been knockout validated in Western blot. The expected band for SOD2 was observed in wild type cells and the band was not seen in knockout cells.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

See full target information Superoxide dismutase [Mn], mitochondrial

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

NPJ systems biology and applications 11:67 PubMed40593928

2025

Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Te Lin,Yi-Ju Lee,Wen-Wei Tseng,Zih-Hua Chen,Huai-Ching Hsieh,Ko-Hong Lin,Jin-Yu Su,An-Chi Wei

Antioxidants (Basel, Switzerland) 14: PubMed40563335

2025

CSE-Induced ER-Mitochondria Crosstalk Promotes Oxidative Stress and Impairs Bronchial Contractile Response.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Rodríguez-Pérez,Rosa Andreu-Martínez,Leila Pérez-Sánchez,Ana Hernández-García,Cecilia Muñoz-Calleja,Ángel Cogolludo,María J Calzada

Cell death discovery 9:16 PubMed36681672

2023

Resveratrol protects osteocytes against oxidative stress in ovariectomized rats through AMPK/JNK1-dependent pathway leading to promotion of autophagy and inhibition of apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Wei,Shuang Chai,Chen Yue,Hong Zhang,Jitian Li,Na Qin

Antioxidants (Basel, Switzerland) 12: PubMed36670868

2022

Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Vladislava A Gusar,Angelika V Timofeeva,Vitaliy V Chagovets,Mikhail Yu Vysokikh,Nataliya E Kan,Ludmila A Manukhova,Maria V Marey,Gennadiy T Sukhikh

Free radical biology & medicine 193:9-22 PubMed36174878

2022

Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase.

Applications

Unspecified application

Species

Unspecified reactive species

J Sevilla-Montero,O Munar-Rubert,J Pino-Fadón,C Aguilar-Latorre,M Villegas-Esguevillas,B Climent,M Agrò,C Choya-Foces,A Martínez-Ruiz,E Balsa,C Muñoz-Calleja,R M Gómez-Punter,E Vázquez-Espinosa,A Cogolludo,M J Calzada

Cell cycle (Georgetown, Tex.) 21:1212-1221 PubMed35244512

2022

Human pulmonary artery smooth muscle cell dysfunction is regulated by miR-509-5p in hypoxic environment.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wang,Rong Jiang,Yanlin Tan,Kuan Cheng

Cell death & disease 13:104 PubMed35110539

2022

Adenine overload induces ferroptosis in human primary proximal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Ali Khan,Purba Nag,Anca Grivei,Kurt T K Giuliani,Xiangju Wang,Vishal Diwan,Wendy Hoy,Helen Healy,Glenda Gobe,Andrew J Kassianos

Journal of cellular physiology 236:6441-6456 PubMed33565085

2021

SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Lin,Jiacheng Du,Xiexing Wu,Congxin Xu,Jiangtao Liu,Luyong Jiang,Xiaoqiang Cheng,Gaoran Ge,Liang Chen,Qingjiang Pang,Dechun Geng,Haiqing Mao

Frontiers in pharmacology 11:944 PubMed32694997

2020

Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Noëmi Johanna Roos,Diell Aliu,Jamal Bouitbir,Stephan Krähenbühl

Molecular cell 77:475-487.e11 PubMed31759822

2019

TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Ferrari,Lara Isabel de Llobet Cucalon,Chiara Di Vona,François Le Dilly,Enrique Vidal,Antonios Lioutas,Javier Quilez Oliete,Laura Jochem,Erin Cutts,Giorgio Dieci,Alessandro Vannini,Martin Teichmann,Susana de la Luna,Miguel Beato

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOD2/MnSOD antibody [mAbcam 74231]

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)

Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

Product promise