Mouse Monoclonal SOD2/MnSOD antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Rat, Human samples. Cited in 4 publications.
Images
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-mabcam-74231-ab74231--western-blot-img95627.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)")
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-mabcam-74231-ab74231--western-blot-img87857.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)")
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-mabcam-74231-ab74231--western-blot-img400259.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (AB74231)")
Publications
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- Antioxidants (Basel, Switzerland) 12:2022Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladislava A Gusar,Angelika V Timofeeva,Vitaliy V Chagovets,Mikhail Yu Vysokikh,Nataliya E Kan,Ludmila A Manukhova,Maria V Marey,Gennadiy T SukhikhPubMed 36670868
- Cell death & disease 13:1042022Adenine overload induces ferroptosis in human primary proximal tubular epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Ali Khan,Purba Nag,Anca Grivei,Kurt T K Giuliani,Xiangju Wang,Vishal Diwan,Wendy Hoy,Helen Healy,Glenda Gobe,Andrew J KassianosPubMed 35110539
- Journal of cellular physiology 236:6441-64562021SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayi Lin,Jiacheng Du,Xiexing Wu,Congxin Xu,Jiangtao Liu,Luyong Jiang,Xiaoqiang Cheng,Gaoran Ge,Liang Chen,Qingjiang Pang,Dechun Geng,Haiqing MaoPubMed 33565085
- Frontiers in pharmacology 11:9442020Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoëmi Johanna Roos,Diell Aliu,Jamal Bouitbir,Stephan KrähenbühlPubMed 32694997
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| Flow Cyt (Intra) | WB | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Predicted |
| Rat | Not recommended | Tested |
| Chimpanzee | Not recommended | Predicted |
| Cow | Not recommended | Predicted |
| Cynomolgus monkey | Not recommended | Predicted |
| Guinea pig | Not recommended | Predicted |
| Horse | Not recommended | Predicted |
| Macaque monkey | Not recommended | Predicted |
| Orangutan | Not recommended | Predicted |
| Pig | Not recommended | Predicted |
| Rabbit | Not recommended | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested |
| Zebrafish | Not recommended | Predicted |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Zebrafish, Cynomolgus monkey, Macaque monkey, Orangutan, Mouse, Chimpanzee, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1 µg/mL | Notes We recommend using 3% milk as the blocking agent in Western Blot. |
Species Rat | Dilution info 1 µg/mL | Notes We recommend using 3% milk as the blocking agent in Western Blot. |
Species Human | Dilution info 1 µg/mL | Notes We recommend using 3% milk as the blocking agent in Western Blot. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Pig, Zebrafish, Cynomolgus monkey, Macaque monkey, Orangutan, Mouse, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Alternative names
SOD2
Recommended products
Mouse Monoclonal SOD2/MnSOD antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Rat, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbcam 74231
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This monoclonal antibody to SOD2 has been knockout validated in Western blot. The expected band for SOD2 was observed in wild type cells and the band was not seen in knockout cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-mabcam-74231-ab74231--western-blot-img95627.jpg "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)")
Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
The Superoxide dismutase 2 protein has a predicted molecular weight of 25-kDa. However, the sequence contains a 24 residue transit peptide at the N-terminus and, when cleaved, the protein has an expected molecular weight of approximately 23-kDa.
We recommend using 3% milk as the blocking agent in Western Blot.
All lanes: Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 5 µg/mL
Lane 1: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lane 2: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lane 3: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 4: Brain (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 23 kDa
Exposure time: 5min
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-mabcam-74231-ab74231--western-blot-img87857.jpg "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)")
Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
All lanes: Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/mL
All lanes: Recombinant Human SOD2/MnSOD protein (ab82656) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Exposure time: 12min
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231), expandable thumbnail](https://content.abcam.com/products/images/sod2-mnsod-antibody-mabcam-74231-ab74231--western-blot-img400259.jpg "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)")
Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
Lane 1: Wild-type HEK-293T cell lysate (40 ug)
Lane 2: Empty Lane
Lane 3: SOD2 knockout HEK-293T cell lysate (40 ug)
Lanes 1 - 3: Merged signal (red and green). Green - ab74231 observed at 25 kDa. Orange - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
Gel type: MES
Blocking buffer: 3% Milk
ab74231 was shown to specifically react with SOD2. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab74231 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 GAPDH (loading control) were diluted 1 μg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/mL
Lane 1: Wild-type HEK-293T cell lysate at 40 µg
Lane 3: SOD2 knockout HEK-293T cell lysate at 40 µg
SecondaryLanes 1 and 3: Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed at 1/10000 dilution
Lanes 1 and 3: Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
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