AB230449

Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody

Name: Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449) | Abcam
Brand: Abcam Limited
SKU: AB230449
Price: 605 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.

View Alternative Names

APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Formalin-fixed, paraffin-embedded human-kidney tissue stained for Sodium/Hydrogen Exchanger 1/NHE-1 using ab230449 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1 stained for Sodium/Hydrogen Exchanger 1/NHE-1 (red) using ab230449 at 1/100 dilution in ICC/IF. Left panel stained with Azami-green.

Flow Cytometry (Intracellular) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Intracellular flow cytometric analysis of intracellular Sodium/Hydrogen Exchanger 1/NHE-1 expression in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transeintly expressing Sodium/Hydrogen Exchanger 1/NHE-1 and Azami green, using Normal rabbit IgG (left) and ab230449 at 1/100 dilution (right).

Supplier Data

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

All lanes:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Myc-tagged Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information.

P19634

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium/Hydrogen Exchanger 1 (NHE-1) also known as NHE transporter or antisodium exchanger plays a significant role in regulating intracellular pH and cell volume by exchanging sodium ions for hydrogen ions across the plasma membrane. NHE-1 is a type of NHE protein with a molecular weight of approximately 85-110 kDa. This transmembrane protein is widely expressed in various tissues including the heart brain kidneys and skeletal muscles where it helps maintain cellular homeostasis.

Biological function summary

NHE-1 impacts cellular processes by modulating pH levels within cells and participating in the regulation of cell proliferation differentiation and apoptosis. It doesn’t function as part of a larger complex but plays an independent role essential to cellular ionic balance. The proper functioning of NHE-1 is critical for the survival and functionality of many cell types as it helps adapt to changes in metabolic and environmental conditions.

Pathways

NHE-1 integrates into key cellular signaling systems such as the mitogen-activated protein kinase (MAPK) pathway and the Apoptosis signaling pathway. In the MAPK pathway NHE-1 interacts with signaling molecules to influence cellular responses to growth signals. Additionally its regulation of intracellular pH and ionic concentrations intersects with pathways involving proteins like Calcineurin which can impact various cellular responses and adaptations.

NHE-1 is heavily linked to several cardiovascular conditions including hypertension and cardiac hypertrophy. Its dysregulation can alter cardiac cell function contributing to the pathology of these diseases. In cancer aberrant NHE-1 activity may lead to increased cell survival and proliferation. The protein also connects to disorders like ischemia where its function influences the role of related proteins like Na+/K+ ATPase further implying NHE-1’s involvement in pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (PubMed : 11350981, PubMed : 11532004, PubMed : 14680478, PubMed : 15035633, PubMed : 15677483, PubMed : 17073455, PubMed : 17493937, PubMed : 22020933, PubMed : 27650500, PubMed : 32130622, PubMed : 7110335, PubMed : 7603840). Exchanges intracellular H(+) ions for extracellular Na(+) in 1 : 1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival (PubMed : 12947095, PubMed : 15096511, PubMed : 22020933, PubMed : 8901634). In addition, can transport lithium Li(+) and also functions as a Na(+)/Li(+) antiporter (PubMed : 7603840). SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (PubMed : 15096511).

See full target information SLC9A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 14: PubMed38255741

2024

Spatial Transcriptomic Profiling of Tetraspanins in Stage 4 Colon Cancer from Primary Tumor and Liver Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Thanawat Suwatthanarak,Kullanist Thanormjit,Tharathorn Suwatthanarak,Onchira Acharayothin,Asada Methasate,Vitoon Chinswangwatanakul,Pariyada Tanjak

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Flow Cytometry (Intracellular) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB230449)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise