Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information. Database link P19634
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (PubMed:11350981, PubMed:11532004, PubMed:14680478, PubMed:15035633, PubMed:15677483, PubMed:17073455, PubMed:17493937, PubMed:22020933, PubMed:27650500, PubMed:32130622, PubMed:7110335, PubMed:7603840). Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival (PubMed:12947095, PubMed:15096511, PubMed:22020933, PubMed:8901634). In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter (PubMed:7603840). SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (PubMed:15096511).
Alternative names
APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1
Recommended products
Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information. Database link P19634
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Sodium/Hydrogen Exchanger 1 (NHE-1) also known as NHE transporter or antisodium exchanger plays a significant role in regulating intracellular pH and cell volume by exchanging sodium ions for hydrogen ions across the plasma membrane. NHE-1 is a type of NHE protein with a molecular weight of approximately 85-110 kDa. This transmembrane protein is widely expressed in various tissues including the heart brain kidneys and skeletal muscles where it helps maintain cellular homeostasis.
- Biological function summary
NHE-1 impacts cellular processes by modulating pH levels within cells and participating in the regulation of cell proliferation differentiation and apoptosis. It doesn’t function as part of a larger complex but plays an independent role essential to cellular ionic balance. The proper functioning of NHE-1 is critical for the survival and functionality of many cell types as it helps adapt to changes in metabolic and environmental conditions.
- Pathways
NHE-1 integrates into key cellular signaling systems such as the mitogen-activated protein kinase (MAPK) pathway and the Apoptosis signaling pathway. In the MAPK pathway NHE-1 interacts with signaling molecules to influence cellular responses to growth signals. Additionally its regulation of intracellular pH and ionic concentrations intersects with pathways involving proteins like Calcineurin which can impact various cellular responses and adaptations.
- Associated diseases and disorders
NHE-1 is heavily linked to several cardiovascular conditions including hypertension and cardiac hypertrophy. Its dysregulation can alter cardiac cell function contributing to the pathology of these diseases. In cancer aberrant NHE-1 activity may lead to increased cell survival and proliferation. The protein also connects to disorders like ischemia where its function influences the role of related proteins like Na+/K+ ATPase further implying NHE-1’s involvement in pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)
All lanes: Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449) at 1/500 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: Myc-tagged Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate
Predicted band size: 91 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)
Formalin-fixed, paraffin-embedded human-kidney tissue stained for Sodium/Hydrogen Exchanger 1/NHE-1 using ab230449 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1 stained for Sodium/Hydrogen Exchanger 1/NHE-1 (red) using ab230449 at 1/100 dilution in ICC/IF. Left panel stained with Azami-green.
Flow Cytometry (Intracellular) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)
Intracellular flow cytometric analysis of intracellular Sodium/Hydrogen Exchanger 1/NHE-1 expression in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transeintly expressing Sodium/Hydrogen Exchanger 1/NHE-1 and Azami green, using Normal rabbit IgG (left) and ab230449 at 1/100 dilution (right).
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