Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for ICC, WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.
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Publications
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- Cancers 15:2022Voltage-Gated Sodium Channel Na1.5 Controls NHE-1-Dependent Invasive Properties in Colon Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOsbaldo Lopez-Charcas,Lucile Poisson,Oumnia Benouna,Roxane Lemoine,Stéphanie Chadet,Adrien Pétereau,Widad Lahlou,Serge Guyétant,Mehdi Ouaissi,Piyasuda Pukkanasut,Shilpa Dutta,Sadanandan E Velu,Pierre Besson,Driffa Moussata,Sébastien RogerPubMed 36612049
- Scientific reports 12:62632022Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHideto Deguchi,Tomoko Yamashita,Nao Hiramoto,Yohei Otsuki,Atsushi Mukai,Morio Ueno,Chie Sotozono,Shigeru Kinoshita,Junji HamuroPubMed 35428816
- Scientific reports 11:142942021Correlation between electrical characteristics and biomarkers in breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wang,Ying Li,Jie Huang,Yan Zhang,Ren Ma,Shunqi Zhang,Tao Yin,Shangmei Liu,Yan Song,Zhipeng LiuPubMed 34253828
- Science advances 7:2021The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Pyo Lee et. al.PubMed 33523987
- European journal of pharmacology 888:1734202020Anti-hypertrophic effect of Na/H exchanger-1 inhibition is mediated by reduced cathepsin B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSadaf Riaz et. al.PubMed 32781168
- Scientific reports 9:64302019The NaH-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca] in the Suprachiasmatic Nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPi-Cheng Cheng et. al.PubMed 31015514
- International journal of molecular medicine 43:1193-12022018Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongbin Yang,Liyan Ma,Peng Wang,Dongjing Yang,Yingna Zhang,Xue Zhao,Jie Lv,Jing Zhang,Zhenxiang Zhang,Feng GaoPubMed 30592266
- Journal of experimental & clinical cancer research 37:2552018Elevated Na/H exchanger 1 (SLC9A1) emerges as a marker for tumorigenesis and prognosis in gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiudong Guan et. al.PubMed 30333031
- Oncology letters 16:6713-67202018Increased expression of Na/H exchanger isoform 1 predicts tumor aggressiveness and unfavorable prognosis in epithelial ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixia Wang et. al.PubMed 30405813
- Cell death & disease 9:2922018The interactome and spatial redistribution feature of Ca receptor protein calmodulin reveals a novel role in invadopodia-mediated invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Li et. al.PubMed 29463791
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information. Database link P19634
Reactivity data
Select an application| ICC | WB | IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected | Tested | Expected |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4 µg/mL | Notes - |
Species Mouse | Dilution info 1-4 µg/mL | Notes - |
Species Rat | Dilution info 1-4 µg/mL | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Species Mouse | Dilution info 1-2 µg/mL | Notes - |
Species Rat | Dilution info 1-2 µg/mL | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (PubMed:11350981, PubMed:11532004, PubMed:14680478, PubMed:15035633, PubMed:15677483, PubMed:17073455, PubMed:17493937, PubMed:22020933, PubMed:27650500, PubMed:32130622, PubMed:7110335, PubMed:7603840). Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival (PubMed:12947095, PubMed:15096511, PubMed:22020933, PubMed:8901634). In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter (PubMed:7603840). SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (PubMed:15096511).
Alternative names
APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1
Recommended products
Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for ICC, WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information. Database link P19634
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Sodium/Hydrogen Exchanger 1 (NHE-1) also known as NHE transporter or antisodium exchanger plays a significant role in regulating intracellular pH and cell volume by exchanging sodium ions for hydrogen ions across the plasma membrane. NHE-1 is a type of NHE protein with a molecular weight of approximately 85-110 kDa. This transmembrane protein is widely expressed in various tissues including the heart brain kidneys and skeletal muscles where it helps maintain cellular homeostasis.
- Biological function summary
NHE-1 impacts cellular processes by modulating pH levels within cells and participating in the regulation of cell proliferation differentiation and apoptosis. It doesn’t function as part of a larger complex but plays an independent role essential to cellular ionic balance. The proper functioning of NHE-1 is critical for the survival and functionality of many cell types as it helps adapt to changes in metabolic and environmental conditions.
- Pathways
NHE-1 integrates into key cellular signaling systems such as the mitogen-activated protein kinase (MAPK) pathway and the Apoptosis signaling pathway. In the MAPK pathway NHE-1 interacts with signaling molecules to influence cellular responses to growth signals. Additionally its regulation of intracellular pH and ionic concentrations intersects with pathways involving proteins like Calcineurin which can impact various cellular responses and adaptations.
- Associated diseases and disorders
NHE-1 is heavily linked to several cardiovascular conditions including hypertension and cardiac hypertrophy. Its dysregulation can alter cardiac cell function contributing to the pathology of these diseases. In cancer aberrant NHE-1 activity may lead to increased cell survival and proliferation. The protein also connects to disorders like ischemia where its function influences the role of related proteins like Na+/K+ ATPase further implying NHE-1’s involvement in pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
Lane 1: Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/mL
Lane 2: Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 2 µg/mL
All lanes: MOLT4 cell lysate at 15 µg
Predicted band size: 91 kDa
Observed band size: 48 kDa, 91 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Nhe-1 antibody (ab67314) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nhe-1 antibody (ab67314) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Nhe-1 antibody (ab67314) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/mL
Lane 1: Mouse heart tissue lysate at 15 µg
Lane 2: Mouse kidney tissue lysate at 15 µg
Lane 3: Mouse skin tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa
Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/mL
All lanes: Rat brain tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa
Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 4 µg/mL
All lanes: K562 cell lysates at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa
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