AB67314

Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody

Name: Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) | Abcam
Brand: Abcam Limited
SKU: AB67314
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for ICC, WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.

View Alternative Names

APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nhe-1 antibody (ab67314) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Nhe-1 antibody (ab67314) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Nhe-1 antibody (ab67314) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.

Supplier Data

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 4 µg/mL

All lanes:

K562 cell lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 91 kDa

Observed band size: 91 kDa

false

Unknown

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Lane 1:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/mL

Lane 2:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 2 µg/mL

All lanes:

MOLT4 cell lysate at 15 µg

Predicted band size: 91 kDa

Observed band size: 48 kDa,91 kDa

false

Supplier Data

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/mL

Lane 1:

Mouse heart tissue lysate at 15 µg

Lane 2:

Mouse kidney tissue lysate at 15 µg

Lane 3:

Mouse skin tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 91 kDa

Observed band size: 91 kDa

false

Supplier Data

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/mL

All lanes:

Rat brain tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 91 kDa

Observed band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-FoFr, ICC, WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information.

P19634

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium/Hydrogen Exchanger 1 (NHE-1) also known as NHE transporter or antisodium exchanger plays a significant role in regulating intracellular pH and cell volume by exchanging sodium ions for hydrogen ions across the plasma membrane. NHE-1 is a type of NHE protein with a molecular weight of approximately 85-110 kDa. This transmembrane protein is widely expressed in various tissues including the heart brain kidneys and skeletal muscles where it helps maintain cellular homeostasis.

Biological function summary

NHE-1 impacts cellular processes by modulating pH levels within cells and participating in the regulation of cell proliferation differentiation and apoptosis. It doesn’t function as part of a larger complex but plays an independent role essential to cellular ionic balance. The proper functioning of NHE-1 is critical for the survival and functionality of many cell types as it helps adapt to changes in metabolic and environmental conditions.

Pathways

NHE-1 integrates into key cellular signaling systems such as the mitogen-activated protein kinase (MAPK) pathway and the Apoptosis signaling pathway. In the MAPK pathway NHE-1 interacts with signaling molecules to influence cellular responses to growth signals. Additionally its regulation of intracellular pH and ionic concentrations intersects with pathways involving proteins like Calcineurin which can impact various cellular responses and adaptations.

NHE-1 is heavily linked to several cardiovascular conditions including hypertension and cardiac hypertrophy. Its dysregulation can alter cardiac cell function contributing to the pathology of these diseases. In cancer aberrant NHE-1 activity may lead to increased cell survival and proliferation. The protein also connects to disorders like ischemia where its function influences the role of related proteins like Na+/K+ ATPase further implying NHE-1’s involvement in pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (PubMed : 11350981, PubMed : 11532004, PubMed : 14680478, PubMed : 15035633, PubMed : 15677483, PubMed : 17073455, PubMed : 17493937, PubMed : 22020933, PubMed : 27650500, PubMed : 32130622, PubMed : 7110335, PubMed : 7603840). Exchanges intracellular H(+) ions for extracellular Na(+) in 1 : 1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival (PubMed : 12947095, PubMed : 15096511, PubMed : 22020933, PubMed : 8901634). In addition, can transport lithium Li(+) and also functions as a Na(+)/Li(+) antiporter (PubMed : 7603840). SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (PubMed : 15096511).

See full target information SLC9A1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107206 PubMed37456829

2023

IDH1 K224 acetylation promotes colorectal cancer via miR-9-5p/NHE1 axis-mediated regulation of acidic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Long Zhao,Changjiang Yang,Yilin Lin,Shan Wang,Yingjiang Ye,Jianyuan Luo,Zhanlong Shen

Cancers 15: PubMed36612049

2022

Voltage-Gated Sodium Channel Na1.5 Controls NHE-1-Dependent Invasive Properties in Colon Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Osbaldo Lopez-Charcas,Lucile Poisson,Oumnia Benouna,Roxane Lemoine,Stéphanie Chadet,Adrien Pétereau,Widad Lahlou,Serge Guyétant,Mehdi Ouaissi,Piyasuda Pukkanasut,Shilpa Dutta,Sadanandan E Velu,Pierre Besson,Driffa Moussata,Sébastien Roger

Scientific reports 12:6263 PubMed35428816

2022

Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hideto Deguchi,Tomoko Yamashita,Nao Hiramoto,Yohei Otsuki,Atsushi Mukai,Morio Ueno,Chie Sotozono,Shigeru Kinoshita,Junji Hamuro

Scientific reports 11:14294 PubMed34253828

2021

Correlation between electrical characteristics and biomarkers in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Ying Li,Jie Huang,Yan Zhang,Ren Ma,Shunqi Zhang,Tao Yin,Shangmei Liu,Yan Song,Zhipeng Liu

Science advances 7: PubMed33523987

2021

The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Pyo Lee,Farid Alisafaei,Kolade Adebawale,Julie Chang,Vivek B Shenoy,Ovijit Chaudhuri

European journal of pharmacology 888:173420 PubMed32781168

2020

Anti-hypertrophic effect of Na/H exchanger-1 inhibition is mediated by reduced cathepsin B.

Applications

Unspecified application

Species

Unspecified reactive species

Sadaf Riaz,Nabeel Abdulrahman,Shahab Uddin,Ayesha Jabeen,Alain P Gadeau,Larry Fliegel,Fatima Mraiche

Scientific reports 9:6430 PubMed31015514

2019

The NaH-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca] in the Suprachiasmatic Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Pi-Cheng Cheng,Hsin-Yi Lin,Ya-Shuan Chen,Ruo-Ciao Cheng,Hung-Che Su,Rong-Chi Huang

International journal of molecular medicine 43:1193-1202 PubMed30592266

2018

Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Dongbin Yang,Liyan Ma,Peng Wang,Dongjing Yang,Yingna Zhang,Xue Zhao,Jie Lv,Jing Zhang,Zhenxiang Zhang,Feng Gao

Journal of experimental & clinical cancer research 37:255 PubMed30333031

2018

Elevated Na/H exchanger 1 (SLC9A1) emerges as a marker for tumorigenesis and prognosis in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xiudong Guan,Lanxin Luo,Gulnaz Begum,Gary Kohanbash,Qingkun Song,Aparna Rao,Nduka Amankulor,Baoshan Sun,Dandan Sun,Wang Jia

Oncology letters 16:6713-6720 PubMed30405813

2018

Increased expression of Na/H exchanger isoform 1 predicts tumor aggressiveness and unfavorable prognosis in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Wang,Xingtao Long,Dong Wang,Meng Lou,Dongling Zou,Rui Chen,Weiqi Nian,Qi Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67314)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise