Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

Rabbit Recombinant Monoclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Mouse, Rat samples.

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Images

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB322349), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB	mIHC
Human	Tested	Not recommended	Not recommended	Expected
Mouse	Tested	Not recommended	Not recommended	Tested
Rat	Tested	Not recommended	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information Slc9a1

Function

Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (By similarity). Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (By similarity).

Alternative names

Nhe1, Sodium/hydrogen exchanger 1, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

Rabbit Recombinant Monoclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28913-51
Purification technique: Affinity purification Protein A
Specificity: The immunogen shares no sequence similarity with NHE-2, NHE-3, NHE-4, NHE-5. Based on the immunogen sequence, we do not expect this antibody to cross-react with the other family members. No cross-reactivity testing has been performed.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse stomach tissue labelling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 dilution (B), CD34 with Anti-CD34 antibody [EPR27431-71] ab316277 at 1/10000 dilution (C) and Cathepsin E with ab322657 at 1/5000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 1/NHE-1 (magenta; Opal™690), anti-CD34 (green; Opal™520) and anti-Cathepsin E (gray; Opal™570) on mouse stomach.
Panel B: anti-Sodium/Hydrogen Exchanger 1/NHE-1 showed membranous staining in epithelium of mouse stomach.
Panel C: anti-CD34 staining endothelium in mouse stomach.
Panel D: anti-Cathepsin E showed cytoplasmic staining in mouse stomach.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab322349, Anti-CD34 antibody [EPR27431-71] ab316277 and ab322657 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat liver.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon (PMID: 36612049).

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human stomach.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse stomach.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat colon (PMID: 8282777).

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat stomach.

The section was incubated with ab322349 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins