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Rabbit Recombinant Monoclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB322349), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB322349), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB322349), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB322349), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB322349), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (By similarity). Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (By similarity).

Alternative names

Slc9a1, Sodium/hydrogen exchanger 1, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

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Rabbit Recombinant Monoclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

Alternative names

Slc9a1, Sodium/hydrogen exchanger 1, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28913-51

Purification technique

Affinity purification Protein A

Specificity

The immunogen shares no sequence similarity with NHE-2, NHE-3, NHE-4, NHE-5. Based on the immunogen sequence, we do not expect this antibody to cross-react with the other family members. No cross-reactivity testing has been performed.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on rat liver.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse cerebrum.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse liver.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human liver.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon (PMID: 36612049).

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human stomach.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse stomach.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon (PMID: 8282777).

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (ab322349)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab322349 at 1/4000 (0.125 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat stomach.

    The section was incubated with ab322349 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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