Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free

13 product images

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  Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  Sodium/Hydrogen Exchanger 3/NHE-3 Multiplex immunohistochemistry staining of Rat small intestine tissue using rabbit Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.

  Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections rat small intestine tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with Anti-MUC2 antibody [EPR23479-47] ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
  DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on rat small intestine.

  Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in rat small intestine.

  Panel C: anti-MUC2 staining goblet cells in rat small intestine.

  Panel D: anti-Eph receptor B2 staining membrane of stem cells in rat small intestine.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, Anti-MUC2 antibody [EPR23479-47] ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• 

  Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  Sodium/Hydrogen Exchanger 3/NHE-3 Multiplex immunohistochemistry staining of Rat colon tissue using rabbit Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.

  Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections rat colon tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with Anti-MUC2 antibody [EPR23479-47] ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
  DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on rat colon.

  Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in rat colon.

  Panel C: anti-MUC2 staining goblet cells in rat colon.

  Panel D: anti-Eph receptor B2 staining membrane of stem cells in rat colon.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, Anti-MUC2 antibody [EPR23479-47] ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• 

  Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  Sodium/Hydrogen Exchanger 3/NHE-3 Multiplex immunohistochemistry staining of Mouse small intestine tissue using rabbit Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.

  Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse small intestine tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with Anti-MUC2 antibody [EPR23479-47] ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
  DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on mouse small intestine.

  Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in mouse small intestine.

  Panel C: anti-MUC2 staining goblet cells in mouse small intestine.

  Panel D: anti-Eph receptor B2 staining membrane of stem cells in mouse small intestine.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, Anti-MUC2 antibody [EPR23479-47] ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• 

  Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  Sodium/Hydrogen Exchanger 3/NHE-3 Multiplex immunohistochemistry staining of Mouse colon tissue using rabbit Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.

  Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse colon tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with Anti-MUC2 antibody [EPR23479-47] ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
  DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on mouse colon.

  Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in mouse colon.

  Panel C: anti-MUC2 staining goblet cells in mouse colon.

  Panel D: anti-Eph receptor B2 staining membrane of stem cells in mouse colon.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, Anti-MUC2 antibody [EPR23479-47] ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on rat liver.The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the brush border in rat kidney.The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Apical staining in rat colon.The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on mouse liver.The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the brush border in mouse kidney (PMID: 8238556).The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Apical staining in mouse colon (PMID:?33904662).The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Frozen sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse liver (fresh) tissue lABeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse kidney (fresh) tissue lABeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining in the brush border membrane of proximal tubular cells on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (ab307366)

  This data was developed using Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse colon (fresh) tissue lABeling Sodium/Hydrogen Exchanger 3/NHE-3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining along the apical margin of surface enterocytes on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.