Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Apical staining in mouse colon (PMID : 33904662).The section was incubated with ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh) tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining along the apical margin of surface enterocytes on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307365 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining in the brush border membrane of proximal tubular cells on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307365 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307365 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse small intestine tissue staining Uridine Phosphorylase 1 with ab325391 at a 1/2000 (0.258 μg/ml) dilution, ab307365 anti-Sodium/Hydrogen Exchanger 3/NHE-3 used at a 1/5000 (0.126 μg/ml) dilution and ab272692 anti-MUC2 used at a 1/2000 (0.26 μg/ml) dilution.

Panel A : anti-Uridine Phosphorylase 1 (green; Opal™690), anti-Sodium/Hydrogen Exchanger 3/NHE-3 (magenta; Opal™520), anti-MUC2 (gray; Opal™570) on mouse small intestine.

Panel B : anti-Uridine Phosphorylase 1 staining cytoplasm and nucleus of epithelium in mouse small intestine.

Panel C : anti-Sodium/Hydrogen Exchanger 3/NHE-3 staining apical and luminal facing edges of surface cells in mouse small intestine.

Panel D : anti-MUC2 staining on goblet cells in mouse small intestine.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325391, ab307365 and ab272692 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on mouse liver.The section was incubated with ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the brush border in rat kidney.The section was incubated with ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the brush border in mouse kidney (PMID : 8238556).The section was incubated with ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on rat liver.The section was incubated with ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Sodium/Hydrogen Exchanger 3/NHE-3 with ab307365 at 1/4000 (0.126 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Apical staining in rat colon.The section was incubated with ab307365 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse small intestine tissue labelling Sodium/Hydrogen Exchanger 3 with ab307365 at 1 : 5000 dilution (B), MUC2 with ab272692 at 1 : 2000 dilution (C) Eph receptor B2 with ab252935 at 1 : 500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on mouse small intestine.

Panel B : anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in mouse small intestine.

Panel C : anti-MUC2 staining goblet cells in mouse small intestine.

Panel D : anti-Eph receptor B2 staining membrane of stem cells in mouse small intestine.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307365, ab272692 and ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections rat colon tissue labelling Sodium/Hydrogen Exchanger 3 with ab307365 at 1 : 5000 dilution (B), MUC2 with ab272692 at 1 : 2000 dilution (C) Eph receptor B2 with ab252935 at 1 : 500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on rat colon.

Panel B : anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in rat colon.

Panel C : anti-MUC2 staining goblet cells in rat colon.

Panel D : anti-Eph receptor B2 staining membrane of stem cells in rat colon.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307365, ab272692 and ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse colon tissue labelling Sodium/Hydrogen Exchanger 3 with ab307365 at 1 : 5000 dilution (B), MUC2 with ab272692 at 1 : 2000 dilution (C) Eph receptor B2 with ab252935 at 1 : 500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on mouse colon.

Panel B : anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in mouse colon.

Panel C : anti-MUC2 staining goblet cells in mouse colon.

Panel D : anti-Eph receptor B2 staining membrane of stem cells in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307365, ab272692 and ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] - BSA and Azide free (AB307366)

This data was developed using ab307365, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections rat small intestine tissue labelling Sodium/Hydrogen Exchanger 3 with ab307365 at 1 : 5000 dilution (B), MUC2 with ab272692 at 1 : 2000 dilution (C) Eph receptor B2 with ab252935 at 1 : 500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on rat small intestine.

Panel B : anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in rat small intestine.

Panel C : anti-MUC2 staining goblet cells in rat small intestine.

Panel D : anti-Eph receptor B2 staining membrane of stem cells in rat small intestine.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307365, ab272692 and ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.