AB242007

Anti-Sodium Iodide Symporter antibody [FP5]

Name: Anti-Sodium Iodide Symporter antibody [FP5] (ab242007) | Abcam
Brand: Abcam Limited
SKU: AB242007

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(5 Publications)

Mouse Monoclonal Sodium Iodide Symporter antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC5A5 aa 450 to C-terminus.

View Alternative Names

NIS, SLC5A5, Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Natrium iodide transporter, Sodium-iodide symporter, Solute carrier family 5 member 5, Na(+)/I(-) symporter

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Formalin-fixed, paraffin-embedded mouse trachea tissue stained for Sodium Iodide Symporter with ab242007 at a 1/1000 dilution for 1 hr at RT in immunohistochemical analysis. Fixation : 10% Formalin Solution for 12-24 hours at RT.

Secondary Antibody : HRP/DAB Detection System : Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain : Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μL for 5 minutes at RT.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Formalin-fixed, paraffin-embedded mouse thyroid tissue stained for Sodium Iodide Symporter with ab242007 at a 1/1000 dilution for 1 hr at RT in immunohistochemical analysis. Fixation : 10% Formalin Solution for 12-24 hours at RT.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Supplier Data

Western blot - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

All lanes:

Western blot - Anti-Sodium Iodide Symporter antibody [FP5] (ab242007) at 1/1000 dilution

All lanes:

Human thyroid lysate

Predicted band size: 68 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FP5

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC5A5 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92911

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Iodide Symporter also called NIS or SLC5A5 actively transports iodide into cells particularly in the thyroid gland. This protein weighs approximately 87 kDa and primarily locates in the thyroid follicular cells. It exists also in lactating mammary glands salivary glands and gastric mucosa. By concentrating iodide in these tissues NIS plays an important role in synthesizing thyroid hormones which are essential for metabolism regulation.

Biological function summary

NIS facilitates iodide uptake into thyroid cells enabling the synthesis of thyroid hormones triiodothyronine (T3) and thyroxine (T4). This protein doesn't work alone; instead it is a part of a larger thyroid hormone synthesis complex interacting with thyroid peroxidase (TPO) and thyroglobulin (TG) to ensure efficient hormone production. Its action depends on the sodium gradient established by the Na+/K+ ATPase pump which drives active transport against the concentration gradient.

Pathways

NIS forms a critical component in the hypothalamic-pituitary-thyroid (HPT) axis essential for maintaining the body's metabolism and energy balance. This axis forms a feedback loop where thyrotropin-releasing hormone (TRH) and thyroid-stimulating hormone (TSH) regulate the expression and activity of NIS. In the iodine metabolism pathway NIS cooperates with pendrin a chloride/iodide transporter establishing a seamless flow of iodide into thyroid follicles for hormone synthesis.

NIS dysfunction relates to thyroid-related conditions such as congenital hypothyroidism and papillary thyroid cancer. In congenital hypothyroidism NIS mutations reduce iodide uptake leading to insufficient thyroid hormone production. Papillary thyroid cancer might exploit NIS for its malignancy but altered regulation hampers effective radioiodine therapy. In these disorders the interplay with proteins like TPO and TG alters the normal hormone synthesis process causing significant health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium : iodide symporter that mediates the transport of iodide into the thyroid gland (PubMed : 12488351, PubMed : 18372236, PubMed : 18708479, PubMed : 20797386, PubMed : 31310151, PubMed : 32084174, PubMed : 8806637, PubMed : 9329364). Can also mediate the transport of chlorate, thiocynate, nitrate and selenocynate (PubMed : 12488351).

See full target information SLC5A5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14209 PubMed38825816

2024

Sevoflurane-induced overexpression of extrasynaptic α5-GABAR via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhun Wang,Jinpeng Dong,Mengxue Zhang,Sixuan Wang,Jiangnan Wu,Shengran Wang,Yuan Luo,Yongan Wang,Yiqing Yin

Clinical and translational medicine 14:e1694 PubMed38797942

2024

Targeting miR-31 represses tumourigenesis and dedifferentiation of BRAF-associated thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peitao Zhang,Lizhao Guan,Wei Sun,Yu Zhang,Yaying Du,Shukai Yuan,Xiaolong Cao,Zhengquan Yu,Qiang Jia,Xiangqian Zheng,Zhaowei Meng,Xingrui Li,Li Zhao

European thyroid journal 12: PubMed37352166

2023

Iodine avidity in papillary and poorly differentiated thyroid cancer is predicted by immunohistochemical and molecular work-up.

Applications

Unspecified application

Species

Unspecified reactive species

Joachim N Nilsson,Jonathan Siikanen,Vincenzo Condello,Kenbugul Jatta,Ravi Saini,Christel Hedman,Catharina Ihre Lundgren,C Christofer Juhlin

Environmental science & technology 57:6072-6084 PubMed37022920

2023

Thyroid-Disrupting Effects of Exposure to Fipronil and Its Metabolites from Drinking Water Based on Human Thyroid Follicular Epithelial Nthy-ori 3-1 Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhui Zhuang,Zhiqiang Jiang,Dawei Chen,Jingguang Li,M James C Crabbe,Meiyue Qiu,Yuxin Zheng,Weidong Qu

Frontiers in pharmacology 12:795386 PubMed35002727

2021

AhR Antagonist Promotes Differentiation of Papillary Thyroid Cancer Regulating circSH2B3/miR-4640-5P/IGF2BP2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ri Sa,Meiliang Guo,Danyan Liu,Feng Guan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Sodium Iodide Symporter antibody [FP5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Western blot - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise