AB199410

Anti-Sodium Iodide Symporter antibody - N-terminal

Name: Anti-Sodium Iodide Symporter antibody - N-terminal (ab199410) | Abcam
Brand: Abcam Limited
SKU: AB199410

(1 Review)

(3 Publications )

Rabbit Polyclonal Sodium Iodide Symporter antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SLC5A5.

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Supplier Data

Western blot - Anti-Sodium Iodide Symporter antibody - N-terminal (AB199410)

8%SDS-PAGE

All lanes:

Western blot - Anti-Sodium Iodide Symporter antibody - N-terminal (ab199410) at 1/450 dilution

Lane 1:

Human colon cancer lysate at 40 µg

Lane 2:

Human ovarian cancer lysate at 40 µg

Predicted band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SLC5A5. The exact immunogen used to generate this antibody is proprietary information.

Q92911

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/450 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Iodide Symporter also called NIS or SLC5A5 actively transports iodide into cells particularly in the thyroid gland. This protein weighs approximately 87 kDa and primarily locates in the thyroid follicular cells. It exists also in lactating mammary glands salivary glands and gastric mucosa. By concentrating iodide in these tissues NIS plays an important role in synthesizing thyroid hormones which are essential for metabolism regulation.

Biological function summary

NIS facilitates iodide uptake into thyroid cells enabling the synthesis of thyroid hormones triiodothyronine (T3) and thyroxine (T4). This protein doesn't work alone; instead it is a part of a larger thyroid hormone synthesis complex interacting with thyroid peroxidase (TPO) and thyroglobulin (TG) to ensure efficient hormone production. Its action depends on the sodium gradient established by the Na+/K+ ATPase pump which drives active transport against the concentration gradient.

Pathways

NIS forms a critical component in the hypothalamic-pituitary-thyroid (HPT) axis essential for maintaining the body's metabolism and energy balance. This axis forms a feedback loop where thyrotropin-releasing hormone (TRH) and thyroid-stimulating hormone (TSH) regulate the expression and activity of NIS. In the iodine metabolism pathway NIS cooperates with pendrin a chloride/iodide transporter establishing a seamless flow of iodide into thyroid follicles for hormone synthesis.

NIS dysfunction relates to thyroid-related conditions such as congenital hypothyroidism and papillary thyroid cancer. In congenital hypothyroidism NIS mutations reduce iodide uptake leading to insufficient thyroid hormone production. Papillary thyroid cancer might exploit NIS for its malignancy but altered regulation hampers effective radioiodine therapy. In these disorders the interplay with proteins like TPO and TG alters the normal hormone synthesis process causing significant health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium : iodide symporter that mediates the transport of iodide into the thyroid gland (PubMed : 12488351, PubMed : 18372236, PubMed : 18708479, PubMed : 20797386, PubMed : 31310151, PubMed : 32084174, PubMed : 8806637, PubMed : 9329364). Can also mediate the transport of chlorate, thiocynate, nitrate and selenocynate (PubMed : 12488351).

See full target information SLC5A5

Alternative Names

NIS, SLC5A5, Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Natrium iodide transporter, Sodium-iodide symporter, Solute carrier family 5 member 5, Na(+)/I(-) symporter

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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

Scientific reports 14:4550 PubMed38402305

2024

High-dose exposure to butylparaben impairs thyroid ultrastructure and function in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Lan Jiang,Sha Li,Yang Zeng,Bo-Tao Zhang,Yu Cao,Tao Li,Jun Jiang

International journal of radiation biology 95:1718-1727 PubMed31486712

2019

The effect of radioiodine treatment on the diseased thyroid gland.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew S Riley,Gordon A G McKenzie,Victoria Green,Giuseppe Schettino,R James A England,John Greenman

BMC cancer 19:259 PubMed30902086

2019

A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Riley,Victoria Green,Ramsah Cheah,Gordon McKenzie,Laszlo Karsai,James England,John Greenman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sodium Iodide Symporter antibody - N-terminal

Western blot - Anti-Sodium Iodide Symporter antibody - N-terminal (AB199410)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (3)

Product promise