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Rabbit Recombinant Monoclonal Sodium/Potassium ATPase alpha 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Protein within Dog ATP1A1.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (AB308329), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (AB308329), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (AB308329), expandable thumbnail
  • Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (AB308329), expandable thumbnail
  • Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (AB308329), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Protein within Dog ATP1A1. The exact immunogen used to generate this antibody is proprietary information. Database link P50997

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Expected
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Expected
Expected
Tested
Drosophila melanogaster
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100.00000 - 1/200.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Rat, Drosophila melanogaster
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat, Drosophila melanogaster
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Drosophila melanogaster
Dilution info
1/500 - 1/3000
Notes

-

Target data

Alternative names

A1A1, AT1A1_HUMAN, ATP1A1, ATPase Na+/K+ transporting alpha 1 polypeptide, ATPase Na+/K+ transporting subunit alpha 1, Atpa-1, BC010319, EC 3.6.3.9, MGC3285, MGC38419, MGC51750, Na K ATPase alpha A catalytic polypeptide, Na K ATPase catalytic subunit alpha A protein, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na+, K+ ATPase alpha subunit, Na+/K+ ATPase alpha 1 subunit, Na+/K+ ATPase 1, Na,K ATPase alpha 1 subunit, Nkaa1b, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump 1, Sodium pump subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-1

Recommended products

Rabbit Recombinant Monoclonal Sodium/Potassium ATPase alpha 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Protein within Dog ATP1A1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Protein within Dog ATP1A1. The exact immunogen used to generate this antibody is proprietary information. Database link P50997
Carrier free
Yes
Clone number
HL114
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The alpha 1 Sodium Potassium ATPase often called Na+/K+ ATPase or Na K ATPase plays a critical role in cellular processes by maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) across the plasma membrane. Also known as ATP1 or anti sodium this enzyme facilitates the active transport of Na+ and K+ ions against their concentration gradients a vital function for cellular homeostasis. The molecular weight of this enzyme is approximately 112 kDa with expression observed in various tissues including neuronal and muscular tissues indicating its importance in both central and peripheral cellular functions.

Biological function summary

The alpha 1 Sodium Potassium ATPase establishes ionic balance and is a component of the larger Na K ATPase complex which comprises multiple subunits. This complex not only aids in nerve impulse transmission but also supports muscle contraction and intracellular fluid regulation. Through its activity it significantly influences cellular volume and internal osmotic conditions thereby affecting overall tissue functionality.

Pathways

Alpha 1 Sodium Potassium ATPase is an important player in ion transport and cellular signaling pathways. It closely interacts with pathways such as the renin-angiotensin-aldosterone system and is vital for maintaining blood pressure homeostasis. The enzyme also interacts with several proteins including ankyrin and phospholemman which modulate its activity and are integral to various signaling cascades involved in cardiovascular and renal function.

Associated diseases and disorders

The dysregulation of alpha 1 Sodium Potassium ATPase activity is linked to conditions like hypertension and congestive heart failure. Malfunctions in this enzyme can lead to improper ionic balance contributing to these diseases. The enzyme interacts with other proteins such as aldosterone synthase in hypertension and these interactions are critical in understanding their pathogenic mechanisms. A greater understanding of these associations may guide therapeutic strategies targeting ion transport abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse kidney and duodenum tissue lysate using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    Immunohistochemical analysis of mouse kidney and duodenum tissue labeling Sodium Potassium ATPase alpha 1 using ab308329 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse kidney tissue lysate. using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    Immunohistochemical analysis of mouse kidney tissue labeling Sodium Potassium ATPase alpha 1 using ab308329 at 1/200 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Immunocytochemistry/ Immunofluorescence staining of A431 cells using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    For immunofluorescence analysis, A431 cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of Sodium Potassium ATPase alpha 1 using ab308329 at 1/500 dilution (green). Blue: Fluoroshield with DAPI

  • Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Western blot staining using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    Samples were seperated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329) at 1/50000 dilution

    Lane 1: Mouse brain tissue at 50 µg

    Lane 2: Rat brain tissue at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 113 kDa

  • Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Western blot staining using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    Samples were seperated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329) at 1/500 dilution

    Lane 1: A431 whole cell extract at 30 µg

    Lane 2: A431 membrane extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 113 kDa

  • Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Western blot staining of Drosophila tissue extract using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    Samples were seperated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329) at 1/30000 dilution

    All lanes: Drosophila tissue extract at 5 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 113 kDa

  • Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329), expandable thumbnail

    Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329)

    Sodium Potassium ATPase alpha 1 Western blot staining using rabbit Anti-Sodium Potassium ATPase alpha 1 antibody

    A431 whole cell and membrane lysates (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (ab308329) diluted at 1/500. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Loading Control (ab308329) at 1/500 dilution

    Lane 1: A431 whole cell lysate at 30 µg

    Lane 2: A431 membrane lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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