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AB308329

Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free

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Rabbit Recombinant Monoclonal Sodium/Potassium ATPase alpha 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Protein within Dog ATP1A1.
6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

For immunofluorescence analysis, A431 cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of Sodium Potassium ATPase alpha 1 using ab308329 at 1/500 dilution (green). Blue : Fluoroshield with DAPI

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

Immunohistochemical analysis of mouse kidney and duodenum tissue labeling Sodium Potassium ATPase alpha 1 using ab308329 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

Immunohistochemical analysis of mouse kidney tissue labeling Sodium Potassium ATPase alpha 1 using ab308329 at 1/200 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)
  • WB

Supplier Data

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (ab308329) at 1/500 dilution

Lane 1:

A431 whole cell extract at 30 µg

Lane 2:

A431 membrane extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)
  • WB

Supplier Data

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (ab308329) at 1/50000 dilution

Lane 1:

Mouse brain tissue at 50 µg

Lane 2:

Rat brain tissue at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)
  • WB

Supplier Data

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (ab308329) at 1/30000 dilution

All lanes:

Drosophila tissue extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL114

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Drosophila melanogaster

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Protein within Dog ATP1A1. The exact immunogen used to generate this antibody is proprietary information.

P50997

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1 Sodium Potassium ATPase often called Na+/K+ ATPase or Na K ATPase plays a critical role in cellular processes by maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) across the plasma membrane. Also known as ATP1 or anti sodium this enzyme facilitates the active transport of Na+ and K+ ions against their concentration gradients a vital function for cellular homeostasis. The molecular weight of this enzyme is approximately 112 kDa with expression observed in various tissues including neuronal and muscular tissues indicating its importance in both central and peripheral cellular functions.
Biological function summary

The alpha 1 Sodium Potassium ATPase establishes ionic balance and is a component of the larger Na K ATPase complex which comprises multiple subunits. This complex not only aids in nerve impulse transmission but also supports muscle contraction and intracellular fluid regulation. Through its activity it significantly influences cellular volume and internal osmotic conditions thereby affecting overall tissue functionality.

Pathways

Alpha 1 Sodium Potassium ATPase is an important player in ion transport and cellular signaling pathways. It closely interacts with pathways such as the renin-angiotensin-aldosterone system and is vital for maintaining blood pressure homeostasis. The enzyme also interacts with several proteins including ankyrin and phospholemman which modulate its activity and are integral to various signaling cascades involved in cardiovascular and renal function.

The dysregulation of alpha 1 Sodium Potassium ATPase activity is linked to conditions like hypertension and congestive heart failure. Malfunctions in this enzyme can lead to improper ionic balance contributing to these diseases. The enzyme interacts with other proteins such as aldosterone synthase in hypertension and these interactions are critical in understanding their pathogenic mechanisms. A greater understanding of these associations may guide therapeutic strategies targeting ion transport abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Sodium/Potassium ATPase alpha 1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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