Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Sodium Potassium ATPase with ab283318 at 1/100 dilution (1μg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Sodium Potassium ATPase with ab283318 at 1/10000 dilution (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human hepatocellular carcinoma. The section was incubated with ab283318 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Sodium Potassium ATPase with ab283318 at 1/100 dilution (10.93 ug/ml), followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Sodium Potassium ATPase with ab283318 at 1/10000 dilution (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human colon. The section was incubated with ab283318 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Sodium Potassium ATPase with ab283318 at 1/10000 (0.109 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat stomach. The section was incubated with ab283318 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Sodium Potassium ATPase with ab283318 at 1/10000 dilution (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse stomach. The section was incubated with ab283318 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sodium Potassium ATPase with ab283318 at 1/10000 dilution (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly membranous staining on mouse kidney. The section was incubated with ab283318 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sodium Potassium ATPase with ab283318 at 1/10000 dilution (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly membranous staining on rat kidney. The section was incubated with ab283318 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• WB

Lab

Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (AB283318)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples of Lanes 3, 4, 6, 8 are non-boiled as boiling may cause protein aggregates.

Exposure time : 1 second

All lanes:

Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) (ab283318) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate prepared from RIPA lysis method at 20 µg

Lanes 2 and 4:

HeLa whole cell lysate prepared from 1% SDS HOT lysis method at 20 µg

Lane 3:

HeLa whole cell lysate prepared from RIPA lysis method at 20 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 6:

RAW264.7 whole cell lysate at 20 µg

Lane 7:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 8:

C6 whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 113 kDa

Observed band size: 100 kDa

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