Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) - Plasma Membrane Loading Control (ab283345)

Rat Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) - Plasma Membrane Loading Control (AB283345), expandable thumbnail

Publications

Key facts

Isotype: IgG2a
Host species: Rat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Expected
Rat	Tested	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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2 products for Alternative Version

Target data

See full target information ATP1A1

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed:29499166, PubMed:30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

Alternative names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

Recommended products

Rat Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype: IgG2a
Host species: Rat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Clone number: EP1845Y
Purification technique: Ion exchange chromatography
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control ab76020). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Associated diseases and disorders

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

Product promise

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9 product images

Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) - Plasma Membrane Loading Control (ab283345)
Sodium Potassium ATPase Western blot staining using rat Anti-Sodium Potassium ATPase antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples of Lanes 3, 4, 6, 8 are non-boiled as boiling may cause protein aggregates.
Exposure time: 1 second
All lanes: Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) - Plasma Membrane Loading Control (ab283345) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate prepared from RIPA lysis method at 20 µg
Lanes 2 and 4: HeLa whole cell lysate prepared from 1% SDS HOT lysis method at 20 µg
Lane 3: HeLa whole cell lysate prepared from RIPA lysis method at 20 µg
Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 6: RAW264.7 whole cell lysate at 20 µg
Lane 7: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 8: C6 whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 113 kDa
Observed band size: 100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human colon. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human hepatocellular carcinoma. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly membranous staining on mouse kidney. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse stomach. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly membranous staining on rat kidney. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat stomach. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Sodium Potassium ATPase with ab283345 at 1/50 (22.18 ug/ml) dilution, followed by Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HeLa cell line. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) (ab283345)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Sodium Potassium ATPase with ab283345 at 1/100 dilution (1ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.