AB353

Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control

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(12 Publications)

Chicken Polyclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for ELISA, WB and reacts with Dog samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Dog ATP1A1.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Dog

Applications

WB, ELISA

applications

Immunogen

Full Length Protein corresponding to Dog ATP1A1. The exact immunogen used to generate this antibody is proprietary information.

P50997

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney]. Cross reactivity against ATPase, Sodium, Potassium from other tissues and species may occur but have not been specifically determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Dog": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107436 PubMed38838775

2024

LRRC8/VRAC volume-regulated anion channels are crucial for hearing.

Applications

Unspecified application

Species

Unspecified reactive species

Deborah A Knecht,Mariia Zeziulia,Mit B Bhavsar,Dmytro Puchkov,Hannes Maier,Thomas J Jentsch

Membranes 13: PubMed37887994

2023

Expression, Function and Trafficking of the Human ABCG2 Multidrug Transporter Containing Mutations in an Unstructured Cytoplasmic Loop.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Mózner,Boglárka Zámbó,Zsuzsa Bartos,Anna Gergely,Kata Sára Szabó,Bálint Jezsó,Ágnes Telbisz,György Várady,László Homolya,Tamás Hegedűs,Balázs Sarkadi

Scientific reports 8:7487 PubMed29749379

2018

Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression.

Applications

ICC/IF

Species

Unspecified reactive species

Boglárka Zámbó,Zsuzsa Bartos,Orsolya Mózner,Edit Szabó,György Várady,Gyula Poór,Márton Pálinkás,Hajnalka Andrikovics,Tamás Hegedűs,László Homolya,Balázs Sarkadi

Cell chemical biology 24:589-597.e5 PubMed28434875

2017

Covalent Protein Labeling at Glutamic Acids.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Martín-Gago,Eyad K Fansa,Michael Winzker,Sandip Murarka,Petra Janning,Carsten Schultz-Fademrecht,Matthias Baumann,Alfred Wittinghofer,Herbert Waldmann

Autophagy 10:1241-55 PubMed24905460

2014

Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Jingyi Li,Tao Zhang,Linzhi Zou,Yi Chen,Kui Wang,Yunlong Lei,Kefei Yuan,Yi Li,Jiang Lan,Lin Cheng,Na Xie,Rong Xiang,Edouard C Nice,Canhua Huang,Yuquan Wei

Molecular and cellular biology 34:1221-33 PubMed24449764

2014

S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Eike-Benjamin Braune,Karin Schuster-Gossler,Marcin Lyszkiewicz,Katrin Serth,Kristina Preusse,Johannes Madlung,Boris Macek,Andreas Krueger,Achim Gossler

Cell calcium 55:78-92 PubMed24439526

2014

Histone deacetylase inhibitor- and PMA-induced upregulation of PMCA4b enhances Ca2+ clearance from MCF-7 breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Varga,Katalin Pászty,Rita Padányi,Luca Hegedűs,Jean-Philippe Brouland,Béla Papp,Agnes Enyedi

Biochimica et biophysica acta 1833:2561-2572 PubMed23830917

2013

A C-terminal di-leucine motif controls plasma membrane expression of PMCA4b.

Applications

ICC/IF

Species

Unspecified reactive species

Géza Antalffy,Katalin Pászty,Karolina Varga,Luca Hegedűs,Ágnes Enyedi,Rita Padányi

Glia 59:882-92 PubMed21438010

2011

Differentiation of induced pluripotent stem cells into functional oligodendrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Marcin Czepiel,Veerakumar Balasubramaniyan,Wandert Schaafsma,Mirjana Stancic,Harald Mikkers,Christian Huisman,Erik Boddeke,Sjef Copray

The Journal of cell biology 171:1061-71 PubMed16344308

2005

The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin.

Applications

ICC/IF

Species

Dog

Yi Qin,Christopher Capaldo,Barry M Gumbiner,Ian G Macara

View all publications

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Product promise

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Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise