AB58475

Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control

Name: Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (ab58475) | Abcam
Brand: Abcam Limited
SKU: AB58475
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

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(1 Review)

(28 Publications)

Rabbit Polyclonal alpha 1 Sodium Potassium ATPase antibody. Plasma Membrane marker. Suitable for WB, IHC-P and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human ATP1A1.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

2 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (AB58475)

ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (AB58475)

All lanes:

Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (ab58475) at 1/500 dilution

Lane 1:

293 cell extracts treated with PMA (125ng/ml, 30mins)

Lane 2:

293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

Predicted band size: 113 kDa

Observed band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ATP1A1. The exact immunogen used to generate this antibody is proprietary information.

P05023

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:2083-2100 PubMed40083708

2025

Thrombospondin 1 Promotes Cytoskeleton Remodeling, Dedifferentiation, and Pulmonary Metastasis through ITGA1 and ITGA6 in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Enjie Xu,Zhen Huang,Kunpeng Zhu,Jianping Hu,Xiaolong Ma,Yongjie Wang,Jiazhuang Zhu,Chunlin Zhang

Journal for immunotherapy of cancer 10: PubMed35764366

2022

Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Mandriani,Eleonora Pellè,Francesco Mannavola,Antonio Palazzo,Renè Massimiliano Marsano,Giuseppe Ingravallo,Gerardo Cazzato,Maria Cecilia Ramello,Camillo Porta,Jonathan Strosberg,Daniel Abate-Daga,Mauro Cives

Viruses 14: PubMed35458528

2022

Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1.

Applications

Unspecified application

Species

Unspecified reactive species

Ilina Bhattacharya,Tejabhiram Yadavalli,David Wu,Deepak Shukla

Clinical and translational medicine 12:e699 PubMed35184403

2022

Persistent high glucose induced EPB41L4A-AS1 inhibits glucose uptake via GCN5 mediating crotonylation and acetylation of histones and non-histones.

Applications

Unspecified application

Species

Unspecified reactive species

Weijie Liao,Naihan Xu,Haowei Zhang,Weifang Liao,Yanzhi Wang,Songmao Wang,Shikuan Zhang,Yuyang Jiang,Weidong Xie,Yaou Zhang

Cellular and molecular life sciences : CMLS 79:64 PubMed35013841

2022

A novel effect of PDLIM5 in α7 nicotinic acetylcholine receptor upregulation and surface expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Lin Li,Chen-Yu Gou,Wen-Hui Wang,Yuan Li,Yu Cui,Jing-Jing Duan,Yuan Chen

International journal of molecular sciences 22: PubMed34681640

2021

Mutant in Aldosterone-Producing Adenoma Leads to Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiro Kobuke,Kenji Oki,Celso E Gomez-Sanchez,Elise P Gomez-Sanchez,Kiyotaka Itcho,Haruya Ohno,Gaku Nagano,Yoko Yoshii,Ryuta Baba,Takaya Kodama,Koji Arihiro,Noboru Hattori,Masayasu Yoneda

Frontiers in neurology 12:591223 PubMed33708168

2021

Venlafaxine Attenuated the Cognitive and Memory Deficit in Mice Exposed to Isoflurane Alone.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Li,Chunhai Zhang

Nature communications 11:4116 PubMed32807793

2020

OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmad Sharanek,Audrey Burban,Matthew Laaper,Emilie Heckel,Jean-Sebastien Joyal,Vahab D Soleimani,Arezu Jahani-Asl

The FEBS journal 287:4747-4766 PubMed32112499

2020

Relieving ferroptosis may partially reverse neurodegeneration of the auditory cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Dan Li,Hai-Ying Sun,Wen-Wen Wang,Han Wu,Wen Kong,Wei-Jia Kong

Frontiers in neuroscience 13:1139 PubMed31736686

2019

Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel A Fischer,Michael L Risner,Abigail L Roux,Lauren K Wareham,Rebecca M Sappington

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (AB58475)

Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (AB58475)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

Product promise