AB300508

Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free

Name: Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (ab300508) | Abcam
Brand: Abcam Limited
SKU: AB300508
Price: 905 USD
Availability: InStock
Rating: 3 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell line - Human, Mouse, Rat samples.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1, Sodium/potassium-transporting ATPase subunit alpha-3, Na(+)/K(+) ATPase alpha-3 subunit, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3, ATP1A3, KIAA0778, ATP1A2, Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Sodium pump subunit alpha-2, ATP1AL2, ATP1A4, Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, Sodium pump subunit alpha-4

16 Images

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with human ATP1A1 or ATP1A2 or ATP1A4 expression vector containing a myc tag. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-172 (human brain glioblastoma) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/50 (10.76 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in A-172 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A-172 (human brain glioblastoma) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human cardiac muscle, particularly on the intercalated discs (PMID : 26109061).The section was incubated with ab300507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human cerebrum.The section was incubated with ab300507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with human ATP1A3 expression vector containing a myc tag. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on rat cardiac muscle, particularly on the intercalated discs. The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/50 (10.76 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membrane and weak cytoplasm staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID : 32301109).The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Supplier Data

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation. The samples are unboiled. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (<a href='/en-us/products/primary-antibodies/sodium-potassium-transporting-atpase-pan-alpha-subunit-antibody-epr25414-249-plasma-membrane-loading-control-ab300507'>ab300507</a>) at 1/5000 dilution

Lane 1:

Human cerebellum tissue lysate

Lane 2:

Mouse brain tissue lysate

Lane 3:

Rat brain tissue lysate

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Supplier Data

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation. The samples are unboiled. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Lane 1:

A-172 (human brain glioblastoma ) whole cell lysate

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Exposure time: 48s

Supplier Data

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

This data was developed using ab300507, the same antibody clone in a different buffer formulation. The samples are unboiled. Bands above 110 kDa are aggregates of Na+/K+-ATPase alpha subunits. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate 10

Lane 2:

HEK-293 transfected with ATP1A1 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

Lane 3:

HEK-293 transfected with ATP1A2 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

Lane 4:

HEK-293 transfected with ATP1A3 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

Lane 5:

HEK-293 transfected with ATP1A4 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Exposure time: 3s

Unconjugated

Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25414-249

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-Fr, Flow Cyt (Intra), ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The sodium/potassium-transporting ATPase also known as Na+/K+ ATPase or the pan alpha subunit functions as an essential pump that exchanges three sodium ions out of the cell for two potassium ions into the cell using ATP as the energy source. This process maintains the electrochemical gradients of sodium and potassium across the plasma membrane. The ATPase has a molecular mass of approximately 112 kDa and consists of multiple isoforms including ATP1A1 ATP1A2 ATP1A3 and ATP1A4 expressed in various tissues such as the brain kidney and heart.

Biological function summary

The Na+/K+ ATPase is part of a larger complex that influences numerous cellular activities including cell volume regulation and secondary active transport. It helps maintain the resting potential of cells and facilitates transport of nutrients and other molecules across cell membranes. This pump’s activity is critical for nerve impulse transmission muscle contraction and kidney function tying it to several essential physiological processes.

Pathways

The Na+/K+ ATPase plays a role in key pathways like the cardiac contractility pathway and the neural activity pathway. In the cardiac contractility pathway it influences heart muscle contraction and is functionally related to proteins such as phospholamban and SERCA. In neural activity it helps generate action potentials and is closely related to the function of voltage-gated sodium channels.

Mutations or malfunctions in the Na+/K+ ATPase can relate to conditions like familial hemiplegic migraine and cardiac arrhythmias. In familial hemiplegic migraine changes in ATP1A2 have been implicated influencing ion homeostasis in neurons. In cardiac arrhythmias ATP1A1 has connections to abnormal ion gradients affecting the rhythm of heartbeats and linking it to other proteins involved in cardiac excitability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

Additional targets

ATP1A4,ATP1A3,ATP1A2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (BSA and Azide free) (AB300508)

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]-BSA&Azide free (AB300508)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise