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Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (AB300507), expandable thumbnail
  • Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (AB300507), expandable thumbnail
  • Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (AB300507), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (AB300507), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (AB300507), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Expected
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Tested
Tested
Not recommended
Rat
Tested
Tested
Tested
Expected
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed:29499166, PubMed:30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

Additional Targets

ATP1A4, ATP1A3, ATP1A2

Alternative names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

Recommended products

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25414-249
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The sodium/potassium-transporting ATPase also known as Na+/K+ ATPase or the pan alpha subunit functions as an essential pump that exchanges three sodium ions out of the cell for two potassium ions into the cell using ATP as the energy source. This process maintains the electrochemical gradients of sodium and potassium across the plasma membrane. The ATPase has a molecular mass of approximately 112 kDa and consists of multiple isoforms including ATP1A1 ATP1A2 ATP1A3 and ATP1A4 expressed in various tissues such as the brain kidney and heart.

Biological function summary

The Na+/K+ ATPase is part of a larger complex that influences numerous cellular activities including cell volume regulation and secondary active transport. It helps maintain the resting potential of cells and facilitates transport of nutrients and other molecules across cell membranes. This pump’s activity is critical for nerve impulse transmission muscle contraction and kidney function tying it to several essential physiological processes.

Pathways

The Na+/K+ ATPase plays a role in key pathways like the cardiac contractility pathway and the neural activity pathway. In the cardiac contractility pathway it influences heart muscle contraction and is functionally related to proteins such as phospholamban and SERCA. In neural activity it helps generate action potentials and is closely related to the function of voltage-gated sodium channels.

Associated diseases and disorders

Mutations or malfunctions in the Na+/K+ ATPase can relate to conditions like familial hemiplegic migraine and cardiac arrhythmias. In familial hemiplegic migraine changes in ATP1A2 have been implicated influencing ion homeostasis in neurons. In cardiac arrhythmias ATP1A1 has connections to abnormal ion gradients affecting the rhythm of heartbeats and linking it to other proteins involved in cardiac excitability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507), expandable thumbnail

    Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507)

    Sodium/potassium-transporting ATPase (pan alpha subunit) Western blot staining using rabbit Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The samples are unboiled.

    All lanes: Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507) at 1/1000 dilution

    Lane 1: A-172 (human brain glioblastoma ) whole cell lysate

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 110 kDa

    Exposure time: 48s

  • Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507), expandable thumbnail

    Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507)

    Sodium/potassium-transporting ATPase (pan alpha subunit) Western blot staining using rabbit Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The samples are unboiled.

    All lanes: Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507) at 1/5000 dilution

    Lane 1: Human cerebellum tissue lysate

    Lane 2: Mouse brain tissue lysate

    Lane 3: Rat brain tissue lysate

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 110 kDa

  • Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507), expandable thumbnail

    Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507)

    Sodium/potassium-transporting ATPase (pan alpha subunit) Western blot staining using rabbit Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The samples are unboiled.Bands above 110 kDa are aggregates of Na+/K+-ATPase alpha subunits.

    All lanes: Western blot - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] - Plasma Membrane Loading Control (ab300507) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate 10

    Lane 2: HEK-293 transfected with ATP1A1 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

    Lane 3: HEK-293 transfected with ATP1A2 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

    Lane 4: HEK-293 transfected with ATP1A3 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

    Lane 5: HEK-293 transfected with ATP1A4 (WT) expression vector containi a myc-His-tag®, whole cell lysate 10

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 110 kDa

    Exposure time: 3s

  • Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/50 (10.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membrane and weak cytoplasm staining in Neuro-2a cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-172 (human brain glioblastoma) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/50 (10.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in A-172 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with human ATP1A1 or ATP1A2 or ATP1A4 expression vector containing a myc tag. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/500 (1.076 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with human ATP1A3 expression vector containing a myc tag. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cardiac muscle, particularly on the intercalated discs. The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID: 32301109).The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum.The section was incubated with ab300507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ATP1A1+ATP1A2+ATP1A3+ATP1A4 with ab300507 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cardiac muscle, particularly on the intercalated discs (PMID: 26109061).The section was incubated with ab300507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

  • Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249] (ab300507)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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