Rabbit Polyclonal Sohlh2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOHLH2 aa 1-200.
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Publications
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- Oncotarget 7:49411-494242016Sohlh2 suppresses epithelial to mesenchymal transition in breast cancer via downregulation of IL-8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufang Ji,Wenfang Zhang,Xiaoli Zhang,Chunyan Hao,Aijun Hao,Qing Gao,Hongying Zhang,Jinhao Sun,Jing HaoPubMed 27384482
- Molecular carcinogenesis 55:1127-372015Sohlh2 inhibits human ovarian cancer cell invasion and metastasis by transcriptional inactivation of MMP9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyu Zhang,Chunyan Hao,Yang Wang,Shufang Ji,Xiaoli Zhang,Wenfang Zhang,Qinghao Zhao,Jinhao Sun,Jing HaoPubMed 26153894
- Carcinogenesis 35:1863-712014Sohlh2 inhibits ovarian cancer cell proliferation by upregulation of p21 and downregulation of cyclin D1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyu Zhang et. al.PubMed 24858206
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SOHLH2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NX45
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Suggested antigen retrieval: use heat mediated 10 mM Citrate buffer or Tris EDTA buffer (pH8.0). |
Target data
Function
Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation. Coordinates oocyte differentiation without affecting meiosis I (By similarity).
Alternative names
TEB1, SOHLH2, Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
Recommended products
Rabbit Polyclonal Sohlh2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOHLH2 aa 1-200.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SOHLH2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NX45
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sohlh2 also known as Spermatogenesis and Oogenesis-specific basic Helix-Loop-Helix 2 is a transcription factor with a molecular mass of about 32 kDa. It is expressed in reproductive tissues notably in the ovaries and testes. Sohlh2 plays a mechanical role by binding to specific DNA sequences regulating the expression of genes essential for germ cell development. This protein influences the differentiation and function of cells critical for reproduction.
- Biological function summary
Sohlh2 influences germline specification and is key for both oogenesis and spermatogenesis. By ensuring the proper expression of specific target genes it plays a pivotal role in reproductive organ development. Sohlh2 could form complexes with other transcription factors although further research may be needed to define such interactions comprehensively. The regulation and presence of Sohlh2 ensure that germ cells undergo proper development and maturation.
- Pathways
Sohlh2 is integrated into reproductive pathways that regulate germ cell differentiation. It interacts through pathways involving gene expression and cellular differentiation. Proteins like Figla and Nobox also participate in these reproductive processes and together with Sohlh2 they orchestrate the development of gametes. These interactions are integral for maintaining fertility and proper reproductive system function.
- Associated diseases and disorders
Sohlh2 has implications in fertility issues and gonadal dysgenesis. Altered expression or mutations in Sohlh2 can contribute to conditions like premature ovarian failure or azoospermia. Disorders involving reproductive development may reflect dysregulation of related proteins such as Lhx8 which works alongside Sohlh2 in the same biological processes. Understanding these interactions and pathways is fundamental for potential therapeutic strategies in treating related fertility disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Sohlh2 antibody (ab101402)
12% SDS PAGE
All lanes: Western blot - Anti-Sohlh2 antibody (ab101402) at 1/1000 dilution
Lane 1: NT2D1 whole cell lysate at 30 µg
Lane 2: MCF7 whole cell lysate at 30 µg
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sohlh2 antibody (ab101402)
Immunohistochemical analysis of paraffin embedded Ca922 xenograft, using ab101402 at a 1/500 dilution.
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