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AB101402

Anti-Sohlh2 antibody

1

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Sohlh2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOHLH2 aa 1 to C-terminus.

View Alternative Names

TEB1, SOHLH2, Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sohlh2 antibody (AB101402)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sohlh2 antibody (AB101402)

Immunohistochemical analysis of paraffin embedded Ca922 xenograft, using ab101402 at a 1/500 dilution.

Western blot - Anti-Sohlh2 antibody (AB101402)
  • WB

Unknown

Western blot - Anti-Sohlh2 antibody (AB101402)

12% SDS PAGE

All lanes:

Western blot - Anti-Sohlh2 antibody (ab101402) at 1/1000 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

MCF7 whole cell lysate at 30 µg

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SOHLH2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NX45

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval: use heat mediated 10 mM Citrate buffer or Tris EDTA buffer (pH8.0).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sohlh2 also known as Spermatogenesis and Oogenesis-specific basic Helix-Loop-Helix 2 is a transcription factor with a molecular mass of about 32 kDa. It is expressed in reproductive tissues notably in the ovaries and testes. Sohlh2 plays a mechanical role by binding to specific DNA sequences regulating the expression of genes essential for germ cell development. This protein influences the differentiation and function of cells critical for reproduction.
Biological function summary

Sohlh2 influences germline specification and is key for both oogenesis and spermatogenesis. By ensuring the proper expression of specific target genes it plays a pivotal role in reproductive organ development. Sohlh2 could form complexes with other transcription factors although further research may be needed to define such interactions comprehensively. The regulation and presence of Sohlh2 ensure that germ cells undergo proper development and maturation.

Pathways

Sohlh2 is integrated into reproductive pathways that regulate germ cell differentiation. It interacts through pathways involving gene expression and cellular differentiation. Proteins like Figla and Nobox also participate in these reproductive processes and together with Sohlh2 they orchestrate the development of gametes. These interactions are integral for maintaining fertility and proper reproductive system function.

Sohlh2 has implications in fertility issues and gonadal dysgenesis. Altered expression or mutations in Sohlh2 can contribute to conditions like premature ovarian failure or azoospermia. Disorders involving reproductive development may reflect dysregulation of related proteins such as Lhx8 which works alongside Sohlh2 in the same biological processes. Understanding these interactions and pathways is fundamental for potential therapeutic strategies in treating related fertility disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation. Coordinates oocyte differentiation without affecting meiosis I (By similarity).
See full target information SOHLH2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:49411-49424 PubMed27384482

2016

Sohlh2 suppresses epithelial to mesenchymal transition in breast cancer via downregulation of IL-8.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Ji,Wenfang Zhang,Xiaoli Zhang,Chunyan Hao,Aijun Hao,Qing Gao,Hongying Zhang,Jinhao Sun,Jing Hao

Molecular carcinogenesis 55:1127-37 PubMed26153894

2015

Sohlh2 inhibits human ovarian cancer cell invasion and metastasis by transcriptional inactivation of MMP9.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyu Zhang,Chunyan Hao,Yang Wang,Shufang Ji,Xiaoli Zhang,Wenfang Zhang,Qinghao Zhao,Jinhao Sun,Jing Hao

Carcinogenesis 35:1863-71 PubMed24858206

2014

Sohlh2 inhibits ovarian cancer cell proliferation by upregulation of p21 and downregulation of cyclin D1.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyu Zhang,Xiaoli Zhang,Shufang Ji,Chunyan Hao,Yulan Mu,Jinhao Sun,Jing Hao
View all publications
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Product promise

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