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AB192873

Anti-Solo antibody [EPR13772-74]

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(1 Publication)

Rabbit Recombinant Monoclonal Solo antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

SOLO, SESTD1, SEC14 domain and spectrin repeat-containing protein 1, Huntingtin-interacting protein-like protein, Protein Solo

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Solo antibody [EPR13772-74] (AB192873)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Solo antibody [EPR13772-74] (AB192873)

Immunofluorescence analysis of Neuro2a cells labeling Solo using ab192873 at 1/50 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor®488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain : DAPI.

Western blot - Anti-Solo antibody [EPR13772-74] (AB192873)
  • WB

Supplier Data

Western blot - Anti-Solo antibody [EPR13772-74] (AB192873)

All lanes:

Western blot - Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Western blot - Anti-Solo antibody [EPR13772-74] (AB192873)
  • WB

Supplier Data

Western blot - Anti-Solo antibody [EPR13772-74] (AB192873)

All lanes:

Western blot - Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13772-74

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Solo' also known as the SQSTM1-like protein is a multi-functional protein with a mass of approximately 62 kDa. It is expressed abundantly in the cytoplasm of various cell types and tissues particularly in muscle and nerve tissues. Solo functions mechanistically by serving as a scaffold protein that interacts with other proteins to regulate cellular processes such as autophagy and signal transduction. Its expression level can vary depending on the cellular context indicating its role in dynamic cellular modulation.
Biological function summary

Solo operates within the context of cellular maintenance and response mechanisms. It acts as an important regulator in autophagic and ubiquitin-proteasome systems often but not always working in conjunction with other proteins to form complexes. Solo modulates the degradation of misfolded proteins and damaged organelles ensuring cellular homeostasis and stress response. Its interactions may influence cellular survival pathways emphasizing Solo's participation in maintaining cellular integrity and normal function.

Pathways

Solo's involvement spans important biological processes such as the autophagy-lysosome pathway and the mTOR signaling pathway. In the autophagy pathway Solo interacts with proteins like LC3 and p62 playing an important role in cargo recognition and transport to lysosomes for degradation. In the mTOR pathway it influences cell growth and proliferation by modulating nutrient sensing and energy balance. Solo's interactions within these pathways highlight its role in regulating cellular metabolism and stress responses.

Solo has been associated with neurodegenerative conditions and muscular dystrophies. In diseases like Alzheimer's Solo's dysregulation can affect protein aggregation and clearance linking it to proteins such as tau and amyloid-beta. In muscular dystrophies alterations in Solo expression and function might impact muscle cell health associating it with dystrophin and related proteins. Understanding Solo's roles in these diseases can provide insights into therapeutic targets and strategies for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as the primary docking protein directing membrane turnover and assembly of the transient receptor potential channels TRPC4 and TRPC5. Binds phospholipids such as phosphatidylinositol monophosphates, phosphatidylinositol diphosphates (PIP2s) and phosphatidic acid, but not less polar lipids including phosphatidylcholine, phosphatidylserine, and phosphatidylinositol. The binding to PIP2s is calcium dependent. Might be involved in the plasma membrane localization of CTNNB1.
See full target information SESTD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:13711-13726 PubMed41064490

2025

Comprehensive Analysis and Experimental Validation of Immune-Related Biomarkers and Immune Microenvironment in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Weini Zhou,Ziyang Zeng,Xunjia Li,Mei Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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