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AB308507

Anti-TNF Receptor I antibody [EPR26198-14] - BSA and Azide free

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Rabbit Recombinant Monoclonal TNF Receptor I antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody [EPR26198-14] - BSA and Azide free (AB308507)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody [EPR26198-14] - BSA and Azide free (AB308507)

This data was developed using ab308506, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling Soluble TNF Receptor I + Fc Fragment with ab308506 at 1/50 (10.52 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line.Negative control : Raji (PMID : 10583368, PMID : 8144934). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Western blot - Anti-TNF Receptor I antibody [EPR26198-14] - BSA and Azide free (AB308507)
  • WB

Supplier Data

Western blot - Anti-TNF Receptor I antibody [EPR26198-14] - BSA and Azide free (AB308507)

This data was developed using 308506, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Raji (PMID : 8144934, PMID : 10583368, PMID : 1966549). The molecular weight observed is consistent with the isoforms. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200,000 dilution. Exposure time : Lane 1 : 92 seconds, Lanes 2-4 : 180 seconds.

All lanes:

Western blot - Anti-TNF Receptor I antibody [EPR26198-14] (<a href='/en-us/products/primary-antibodies/soluble-tnf-receptor-i-fc-fragment-antibody-epr26198-14-ab308506'>ab308506</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate, at 20 µg

Lane 2:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate, at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate, at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 55 kDa,38 kDa,28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26198-14

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Soluble TNF Receptor I + Fc Fragment also known as TNFRSF1A-Fc is a recombinant fusion protein with an approximate mass of 75 kDa. Mechanically this fusion protein acts by binding to tumor necrosis factor-alpha (TNF-α) inhibiting its activity and preventing interactions with cell surface TNF receptors. Soluble TNF Receptor I + Fc Fragment keeps the TNF-α activity in check by acting as a decoy receptor. It is expressed in various cells including immune cells and endothelial cells allowing it to modulate inflammatory responses effectively.
Biological function summary

The Soluble TNF Receptor I + Fc Fragment helps regulate immune and inflammatory responses. It functions by blocking the pro-inflammatory effects of TNF-α an element pivotal in the inflammatory process. By neutralizing TNF-α it prevents the recruitment and activation of immune cells and cytokines. This protein does not function alone; it works within larger protein complexes to execute its role in cellular communication and signal transduction.

Pathways

This fusion protein plays a significant role in the NF-κB and apoptosis signal pathways. Within these pathways TNF-α is an important mediator whose activity is moderated by the presence of the Soluble TNF Receptor I + Fc Fragment. By inhibiting TNF-induced activation of NF-κB the fusion protein can influence processes related to cell survival and death. It is closely related to proteins such as IκB (inhibitor of NF-κB) showing how its activity can have downstream effects on a variety of signaling processes.

Soluble TNF Receptor I + Fc Fragment is used therapeutically in conditions like rheumatoid arthritis and inflammatory bowel disease. These disorders involve high levels of TNF-α which contribute to excessive inflammatory responses. The fusion protein helps mitigate such conditions by decreasing the TNF-α activity which in turn lowers inflammation. Through these actions this target is also indirectly connected to the regulatory proteins involved in these disorders like interleukin-6 (IL-6) which can also influence the inflammatory process.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
See full target information TNFRSF1A
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Product promise

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For full details, please see our Terms & Conditions

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