Anti-Somatostatin 28 antibody [EPR3359(2)]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Carboxypeptidase A (ab278044, gray; Opal™690), anti-GIP (ab271989, green; Opal™520) and anti-Somatostatin 28 (ab111912, red; Opal™570) on human pancreas. Panel B : anti-Carboxypeptidase A stained on acinar cells. Panel C : anti-GIP stained on alpha cells. Panel D : anti-Somatostatin 28 stained on delta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab278044 at 1/4000 dilution (0.135 μg/ml), ab271989 at 1/4000 dilution (0.27 μg/ml) and ab111912 at 1/9000 dilution (0.068 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin 28 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody at 1/4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of human pancreas islet. [PMID : 25019573], [PMID : 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912, at a 1/50 dilution, staining Somatostatin 28 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin 28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated in a TRIS/EDTA buffer pH9 (ab93684).Primary antibody at 1 : 4000 dilution and Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of rat pancreas islet[PMID : 25019573], [PMID : 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin-28 in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100.Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1 : 500 dilution. ab150077 (1 : 1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of mouse hippocampus [PMID : 25469233].

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin-28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).Primary antibody was used at a 1 : 500 dilution. ab150077 (1 : 1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining on delta cells of rat pancreas islet. [PMID : 25019573], [PMID : 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin-28 in Rat hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1 : 500 dilution. ab150077 (1 : 1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of rat hippocampus [PMID : 25469233] .

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Immunohistochemical analysis of paraffin-embedded (A) Hippocampus tissue from wild-type C57BL/6JGpt mice and (B) Hippocampus tissue from SST knockout mice tissue labeling Somatostatin 28 with ab111912 at 1/3000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on (A) Hippocampus tissue from wild-type C57BL/6JGpt mice and no staining on (B) Hippocampus tissue from SST knockout mice.
The section was incubated with ab111912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID : T014160).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Immunohistochemical analysis of paraffin-embedded (A) Brain tissue from wild-type C57BL/6JGpt mice and (B) Brain tissue from SST knockout mice tissue labeling Somatostatin 28 with ab111912 at 1/3000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on (A) Brain tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brain tissue from SST knockout mice.
The section was incubated with ab111912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID : T014160).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Immunohistochemical analysis of paraffin-embedded (A) Pancreas tissue from wild-type C57BL/6JGpt mice and (B) Pancreas tissue from SST knockout mice tissue labeling Somatostatin 28 with ab111912 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on (A) Pancreas tissue from wild-type C57BL/6JGpt mice and no staining on (B) Pancreas tissue from SST knockout mice.
The section was incubated with ab111912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID : T014160).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A : merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
Panel B : anti-Somatostatin 28 staining the delta cells in rat pancreas islet.
Panel C : anti-GIP staining the alpha cells in rat pancreas islet.
Panel D : anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.

Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin 28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody at 1 : 4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Couterstained with hematoxylin. Cytoplasmic staining on delta cells of mouse pancreas islet [PMID : 25019573], [PMID : 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Fluorescence multiplex immunohistochemical analysis of the mouse pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A : merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on mouse pancreas.
Panel B : anti-Somatostatin 28 staining the delta cells in mouse pancreas islet.
Panel C : anti-GIP staining the alpha cells in mouse pancreas islet.
Panel D : anti-Pancreatic Polypeptide staining the PP cells in mouse pancreas islet.

Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

ab111912 staining Somatostatin-28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1 : 500 dilution. ab150077 (1 : 1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining on delta cells of mouse pancreas islet. [PMID : 25019573], [PMID : 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912)

Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue.

Panel A : Merged staining of anti-Pancreatic Polypeptide (ab255827, green), anti-Somatostatin 28 (ab111912, red) and DAPI (blue).

Panel B : Anti-Pancreatic Polypeptide (green) stained on PP cells in rat pancreas islet.

Panel C : Anti-Somatostatin 28 (red) stained on delta cells in rat pancreas islet.

Panel D : Nuclear counter stain, DAPI (blue).

Key protocol steps : The section was incubated in two rounds of staining with ab255827 (1/20000 dilution) and ab111912 (1/2000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore. Heat mediated antigen retrieval was used (Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins after every round of antibody/fluorophore staining.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.