Name: Anti-Somatostatin 28 antibody [EPR3359(2)]
SKU: ab111912
Rating: 5 (4 reviews)

Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912) is a rabbit monoclonal antibody detecting Somatostatin 28 in IHC-P, IHC-Fr, mIHC. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents

- Biophysical QC for unrivalled batch-batch consistency

Related conjugates and formulations

ab227422
Carrier free
Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free
ab313257
Conjugated
Alexa Fluor® 555 Anti-Somatostatin 28 antibody [EPR3359(2)]
ab311776
Conjugated
Alexa Fluor® 594 Anti-Somastatin 28 antibody [EPR3359(2)]
ab311139
Conjugated
Alexa Fluor® 647 Anti-Somatostatin 28 antibody [EPR3359(2)]
ab311011
Conjugated
Alexa Fluor® 488 Anti-Somatostatin 28 antibody [EPR3359(2)]
ab313057
Conjugated
Alexa Fluor® 568 Anti-Somatostatin 28 antibody [EPR3359(2)]
ab320968
Conjugated
Alexa Fluor® 750 Anti-Somatostatin 28 antibody [EPR3359(2)]

Images

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (AB111912), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	Flow Cyt	ICC/IF	IHC-Fr	IHC-P	WB	IP
Human	Tested	Not recommended	Not recommended	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Not recommended	Not recommended	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Not recommended	Not recommended	Tested	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/9000	Notes -
Species Mouse	Dilution info 1/9000	Notes -
Species Rat	Dilution info 1/2000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/50 - 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/3000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

See full target information Somatostatin

Function

Somatostatin-14. Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin. May enhance low-glucose-induced glucagon release by pancreatic alpha cells (By similarity). This effect may be mediated by binding to GPR107 and PKA activation (By similarity). May regulate cardiac contractile function (By similarity). May compromise cardiomyocyte viability (By similarity). In the central nervous system, may impair memory retention and may affect hippocampal excitability (By similarity). May also have anxiolytic and anorexigenic effects (By similarity). May play a role in arterial pressure regulation (By similarity). May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH (PubMed:29615476).

Additional Targets

Somatostatin 28

Alternative names

Somatostatin, Growth hormone release-inhibiting factor, SST

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR3359(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912) has been confirmed by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) testing in Somatostatin Knockout Mouse Brain samples.

Other related products

We have a range of other formats of antibody clone [EPR3359(2)] also available for your convenience:
ab111912, Carrier free - ab227422, Alexa Fluor® 488 - ab311011, Alexa Fluor® 647 - ab311139, Alexa Fluor® 594 - ab311776, Alexa Fluor® 568 - ab313057, Alexa Fluor® 555 - ab313257, Alexa Fluor® 750 - ab320968

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Somatostatin 28 also known as SST-28 is a biologically active peptide that functions as a hormone. It is an alternate form of somatostatin the other being somatostatin-14. Somatostatin 28 is characterized by its longer peptide chain with a molecular mass of approximately 3.2 kDa. This peptide is expressed mainly in the central nervous system particularly in the hypothalamus and in the gastrointestinal tract. Its synthesis involves specific posttranslational processing steps and it acts in various cellular locations performing regulatory roles in endocrine and exocrine systems.

Biological function summary

Somatostatin 28 plays an important role in inhibiting the release of certain hormones and neurotransmitters. It exerts its effects by binding to specific somatostatin receptors which are part of a family of G-protein-coupled receptors. These receptors when activated by somatostatin 28 can inhibit adenylyl cyclase activity reducing cyclic AMP levels and therefore suppressing hormone secretion. While somatostatin 28 does not form part of a large multi-protein complex it acts collaboratively with related peptides and receptors to modulate neuronal and digestive functions.

Pathways

Somatostatin 28 participates centrally in the regulation of the endocrine signaling pathways notably influencing the growth hormone inhibitory pathway. This pathway involves interconnected proteins such as growth hormone-releasing hormone (GHRH) which somatostatin 28 suppresses. Additionally the peptide plays a role in modulating the insulin signaling pathway where it inhibits the secretion of insulin. These pathways highlight somatostatin 28's regulatory influence on metabolism and cellular growth by interacting with other proteins like insulin and GHRH.

Associated diseases and disorders

Somatostatin 28 is implicated in conditions such as acromegaly and certain types of diabetes. In acromegaly excessive growth hormone production often due to pituitary adenomas is counteracted by somatostatin 28's inhibitory effects. The peptide's interaction with the receptor-bound growth hormone contributes to potential therapeutic approaches to managing this disorder. In diabetes specifically type 2 somatostatin 28's role in inhibiting insulin release provides insight into its involvement in glucose metabolism dysregulation. Understanding these connections highlights the importance of somatostatin 28 and related proteins like insulin in the pathophysiology of metabolic diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue.
Panel A: Merged staining of anti-Pancreatic Polypeptide (Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827, green), anti-Somatostatin 28 (ab111912, red) and DAPI (blue).
Panel B: Anti-Pancreatic Polypeptide (green) stained on PP cells in rat pancreas islet.
Panel C: Anti-Somatostatin 28 (red) stained on delta cells in rat pancreas islet.
Panel D: Nuclear counter stain, DAPI (blue).
Key protocol steps: The section was incubated in two rounds of staining with Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 (1/20000 dilution) and ab111912 (1/2000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore. Heat mediated antigen retrieval was used (Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins after every round of antibody/fluorophore staining.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912, at a 1/50 dilution, staining Somatostatin 28 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin-28 in Rat hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of rat hippocampus [PMID: 25469233] .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin 28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Primary antibody at 1:4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Couterstained with hematoxylin. Cytoplasmic staining on delta cells of mouse pancreas islet [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .
Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin-28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).Primary antibody was used at a 1:500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining on delta cells of rat pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .
Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin-28 in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100.Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of mouse hippocampus [PMID: 25469233].
Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin-28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining on delta cells of mouse pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin 28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated in a TRIS/EDTA buffer pH9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684).Primary antibody at 1:4000 dilution and Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of rat pancreas islet[PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
ab111912 staining Somatostatin 28 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Primary antibody at 1/4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of human pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36 No. 1-2 24-32] .
Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Carboxypeptidase A (Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, gray; Opal™690), anti-GIP (Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989, green; Opal™520) and anti-Somatostatin 28 (ab111912, red; Opal™570) on human pancreas. Panel B: anti-Carboxypeptidase A stained on acinar cells. Panel C: anti-GIP stained on alpha cells. Panel D: anti-Somatostatin 28 stained on delta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 dilution (0.135 μg/ml), Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989 at 1/4000 dilution (0.27 μg/ml) and ab111912 at 1/9000 dilution (0.068 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Immunohistochemical analysis of paraffin-embedded (A) Hippocampus tissue from wild-type C57BL/6JGpt mice and (B) Hippocampus tissue from SST knockout mice tissue labeling Somatostatin 28 with ab111912 at 1/3000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Hippocampus tissue from wild-type C57BL/6JGpt mice and no staining on (B) Hippocampus tissue from SST knockout mice.

The section was incubated with ab111912 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID: T014160).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Immunohistochemical analysis of paraffin-embedded (A) Brain tissue from wild-type C57BL/6JGpt mice and (B) Brain tissue from SST knockout mice tissue labeling Somatostatin 28 with ab111912 at 1/3000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Brain tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brain tissue from SST knockout mice.

The section was incubated with ab111912 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^®
RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID: T014160).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Fluorescence multiplex immunohistochemical analysis of the mouse pancreas (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on mouse pancreas.

Panel B: anti-Somatostatin 28 staining the delta cells in mouse pancreas islet.

Panel C: anti-GIP staining the alpha cells in mouse pancreas islet.

Panel D: anti-Pancreatic Polypeptide staining the PP cells in mouse pancreas islet.
Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining: in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989 at 1/4000 dilution (0.25 μg/ml) and Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.

Panel B: anti-Somatostatin 28 staining the delta cells in rat pancreas islet.

Panel C: anti-GIP staining the alpha cells in rat pancreas islet.

Panel D: anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.
Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining: in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989 at 1/4000 dilution (0.25 μg/ml) and Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
Immunohistochemical analysis of paraffin-embedded (A) Pancreas tissue from wild-type C57BL/6JGpt mice and (B) Pancreas tissue from SST knockout mice tissue labeling Somatostatin 28 with ab111912 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Pancreas tissue from wild-type C57BL/6JGpt mice and no staining on (B) Pancreas tissue from SST knockout mice.

The section was incubated with ab111912 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^®
RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID: T014160).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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Anti-Somatostatin 28 antibody [EPR3359(2)]

Images

Publications

Key facts

Immunogen

Reactivity data

mIHC

TestedTested

Flow Cyt

Not recommendedNot recommended

ICC/IF

Not recommendedNot recommended

IHC-Fr

TestedTested

IHC-P

TestedTested

WB

Not recommendedNot recommended

IP

Not recommendedNot recommended

Associated Products

2 products for Alternative Product

Target data

Function

Additional Targets

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

15 product images

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Downloads

Product protocols

Tested
Tested

Not recommended
Not recommended

Not recommended
Not recommended

Tested
Tested

Tested
Tested

Not recommended
Not recommended

Not recommended
Not recommended