Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)

Rabbit Recombinant Multiclonal Somatostatin antibody. Carrier free. Suitable for IHC-P, IHC-Fr, ICC/IF, mIHC, Flow Cyt (Intra) and reacts with Human, Rat, Transfected cell line, Mouse samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (AB315107), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IHC-Fr	ICC/IF	mIHC	Flow Cyt (Intra)	WB
Human	Tested	Expected	Expected	Tested	Expected	Not recommended
Mouse	Tested	Tested	Tested	Expected	Tested	Not recommended
Rat	Tested	Tested	Tested	Expected	Tested	Not recommended
Transfected cell line	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Transfected cell line	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell line	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Somatostatin

Function

Somatostatin-14. Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin. May enhance low-glucose-induced glucagon release by pancreatic alpha cells (By similarity). This effect may be mediated by binding to GPR107 and PKA activation (By similarity). May regulate cardiac contractile function (By similarity). May compromise cardiomyocyte viability (By similarity). In the central nervous system, may impair memory retention and may affect hippocampal excitability (By similarity). May also have anxiolytic and anorexigenic effects (By similarity). May play a role in arterial pressure regulation (By similarity). May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH (PubMed:29615476).

Alternative names

Somatostatin, Growth hormone release-inhibiting factor, SST

Recommended products

Rabbit Recombinant Multiclonal Somatostatin antibody. Carrier free. Suitable for IHC-P, IHC-Fr, ICC/IF, mIHC, Flow Cyt (Intra) and reacts with Human, Rat, Transfected cell line, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1087
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315107 is the carrier-free version of Anti-Somatostatin 28 antibody [RM1087] ab315106.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Somatostatin 28 also known as SST-28 is a biologically active peptide that functions as a hormone. It is an alternate form of somatostatin the other being somatostatin-14. Somatostatin 28 is characterized by its longer peptide chain with a molecular mass of approximately 3.2 kDa. This peptide is expressed mainly in the central nervous system particularly in the hypothalamus and in the gastrointestinal tract. Its synthesis involves specific posttranslational processing steps and it acts in various cellular locations performing regulatory roles in endocrine and exocrine systems.

Biological function summary

Somatostatin 28 plays an important role in inhibiting the release of certain hormones and neurotransmitters. It exerts its effects by binding to specific somatostatin receptors which are part of a family of G-protein-coupled receptors. These receptors when activated by somatostatin 28 can inhibit adenylyl cyclase activity reducing cyclic AMP levels and therefore suppressing hormone secretion. While somatostatin 28 does not form part of a large multi-protein complex it acts collaboratively with related peptides and receptors to modulate neuronal and digestive functions.

Pathways

Somatostatin 28 participates centrally in the regulation of the endocrine signaling pathways notably influencing the growth hormone inhibitory pathway. This pathway involves interconnected proteins such as growth hormone-releasing hormone (GHRH) which somatostatin 28 suppresses. Additionally the peptide plays a role in modulating the insulin signaling pathway where it inhibits the secretion of insulin. These pathways highlight somatostatin 28's regulatory influence on metabolism and cellular growth by interacting with other proteins like insulin and GHRH.

Associated diseases and disorders

Somatostatin 28 is implicated in conditions such as acromegaly and certain types of diabetes. In acromegaly excessive growth hormone production often due to pituitary adenomas is counteracted by somatostatin 28's inhibitory effects. The peptide's interaction with the receptor-bound growth hormone contributes to potential therapeutic approaches to managing this disorder. In diabetes specifically type 2 somatostatin 28's role in inhibiting insulin release provides insight into its involvement in glucose metabolism dysregulation. Understanding these connections highlights the importance of somatostatin 28 and related proteins like insulin in the pathophysiology of metabolic diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

18 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Brain tissue from wild-type C57BL/6JGpt mice and (B) Brain tissue from SST knockout mice tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Brain tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brain tissue from SST knockout mice.

The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID: T014160).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Pancreas tissue from wild-type C57BL/6JGpt mice and (B) Pancreas tissue from SST knockout mice tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Pancreas tissue from wild-type C57BL/6JGpt mice and no staining on (B) Pancreas tissue from SST knockout mice.

The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID: T014160).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Hippocampus tissue from wild-type C57BL/6JGpt mice and (B) Hippocampus tissue from SST knockout mice tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Hippocampus tissue from wild-type C57BL/6JGpt mice and no staining on (B) Hippocampus tissue from SST knockout mice.

The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and SST-KO homozygous mice (Strain ID: T014160).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat liver. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a SST expression vector containing a EGFP tag. (B) HEK-293T transfected with empty vector containing a EGFP tag. tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a SST expression vector containing a EGFP tag. No staining on (B) HEK-293T transfected with empty vector containing a EGFP tag. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat pancreatic islet. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse pancreatic islet. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat cortex primary neuron cells labelling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human pancreatic islet. The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse cortex primary neuron cells labelling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/50 dilution (1 ug, Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Left) isotype control .
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas labelling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/7500 dilution (0.069 μg/ml) (B), GIP with Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989 at 1/4000 dilution (0.27 μg/ml) (C) and Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 dilution (0.135 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain.
Panel A: merged staining of anti-Carboxypeptidase A (gray; Opal™690), anti-GIP (green; Opal™520) and anti-Somatostatin 28 (red; Opal™570) on human pancreas.
Panel B: anti-Somatostatin 28 stained on delta cells.
Panel C: anti-GIP stained on alpha cells.
Panel D: anti-Carboxypeptidase A stained on acinar cells.
The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989, and Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas (fresh) tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/500 (1.03 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on rat pancreatic islet The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas (fresh) tissue labeling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/500 (1.03 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on mouse pancreatic islet. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Somatostatin 28 antibody [RM1087] ab315106 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat cortex primary neuron cells labelling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/100 (5.15 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic staining in rat cortex primary neuron cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Somatostatin 28 antibody [RM1087] - BSA and Azide free (ab315107)
This data was developed using Anti-Somatostatin 28 antibody [RM1087] ab315106, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse cortex primary neuron cells labelling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/100 (5.15 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic staining in mouse cortex primary neuron cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.