AB140665

Anti-Somatostatin antibody [7G5]

Name: Anti-Somatostatin antibody [7G5] (ab140665) | Abcam
Brand: Abcam Limited
SKU: AB140665
Price: 500 USD
Availability: InStock
Rating: 2 (2 reviews)

(2 Reviews)

(2 Publications)

Mouse Monoclonal Somatostatin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Somatostatin.

View Alternative Names

Somatostatin, Growth hormone release-inhibiting factor, SST

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (AB140665)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (AB140665)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Somatostatin with ab140665 at a 1/200 dilution followed by DAB staining.

Flow Cytometry - Anti-Somatostatin antibody [7G5] (AB140665)

Flow Cyt

Unknown

Flow Cytometry - Anti-Somatostatin antibody [7G5] (AB140665)

Flow cytometric analysis of HepG2 cells labelling Somatostatin with ab140665 at 1/200 dilution (green); negative control (red).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (AB140665)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Somatostatin with ab140665 at a 1/200 dilution followed by DAB staining.

Unknown

Western blot - Anti-Somatostatin antibody [7G5] (AB140665)

Expected MW is 38.2 kDa.

All lanes:

Western blot - Anti-Somatostatin antibody [7G5] (ab140665) at 1/500 dilution

All lanes:

Somatostatin recombinant protein

Predicted band size: 13 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Somatostatin.

P61278

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Somatostatin also known as growth hormone-inhibiting hormone (GHIH) is a peptide hormone with a molecular mass around 3200 Daltons. This protein is expressed mainly in the central nervous system including the hypothalamus as well as in the pancreas and gastrointestinal tract. Somatostatin consists of two biologically active forms: one with 14 amino acids and another with 28 amino acids. This target exerts its mechanical function by binding to specific G-protein-coupled somatostatin receptors leading to inhibition of various signaling pathways.

Biological function summary

Somatostatin regulates the endocrine system and affects neurotransmission and cell proliferation. It inhibits the secretion of several hormones such as growth hormone thyroid-stimulating hormone and insulin. Somatostatin also plays a role in reducing gastric acid secretion and slowing down gastrointestinal motility. While somatostatin does not act as part of a larger complex its action is highly localized in the tissues where it is produced and released.

Pathways

Somatostatin acts significantly in both the growth hormone and insulin signaling pathways. In the growth hormone pathway it serves as a counter-regulatory hormone to growth hormone-releasing hormone (GHRH) therefore controlling growth hormone levels. In the insulin signaling pathway somatostatin interacts with insulin and glucagon modulating glucose metabolism. It is also related to other proteins like cortistatin which shares similar functions and can bind to the same receptors.

Somatostatin is linked with conditions such as acromegaly and diabetes. In acromegaly excessive growth hormone production occurs due to reduced somatostatin activity making somatostatin analogs a therapeutic option. Meanwhile in diabetes altered somatostatin levels can influence insulin and glucagon balance affecting glucose homeostasis. Somatostatin’s interaction with its receptors and proteins like insulin plays an important role in the management of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Somatostatin-14. Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin.. Neuronostatin. May enhance low-glucose-induced glucagon release by pancreatic alpha cells (By similarity). This effect may be mediated by binding to GPR107 and PKA activation (By similarity). May regulate cardiac contractile function (By similarity). May compromise cardiomyocyte viability (By similarity). In the central nervous system, may impair memory retention and may affect hippocampal excitability (By similarity). May also have anxiolytic and anorexigenic effects (By similarity). May play a role in arterial pressure regulation (By similarity). May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH (PubMed : 29615476).

See full target information Somatostatin

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pflugers Archiv : European journal of physiology 471:633-645 PubMed30767071

2019

The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic β-cell in rodent.

Applications

Unspecified application

Species

Unspecified reactive species

Israa Mohammad Al-Amily,Pontus Dunér,Leif Groop,Albert Salehi

Journal of cell science 129:2462-71 PubMed27173492

2016

An optical method to evaluate both mass and functional competence of pancreatic α- and β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Chengsheng Han,Wenzhen Zhu,Zhengxing Wu,Yanmei Liu,Liangyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Somatostatin antibody [7G5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (AB140665)

Flow Cytometry - Anti-Somatostatin antibody [7G5] (AB140665)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (AB140665)

Western blot - Anti-Somatostatin antibody [7G5] (AB140665)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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