Anti-Somatostatin antibody ab108456 is a rabbit polyclonal antibody that is used in Somatostatin IHC. Suitable for human, mouse and rat samples.
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- Data in brief 48:1092132023Experimental datasets on the immunohistological assessment of δ-cells in the islet organs of the endocrine pancreas of Japanese medaka () fish exposed to graphene oxide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsok K Dasmahapatra,Paul B TchounwouPubMed 37383828
- Ecotoxicology and environmental safety 253:1146492023Evaluation of pancreatic δ- cells as a potential target site of graphene oxide toxicity in Japanese medaka (Oryzias latipes) fish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsok K Dasmahapatra,Paul B TchounwouPubMed 36806823
- Scientific reports 13:21642023Limbic progesterone receptors regulate spatial memory.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuchitra Joshi,Cedric L Williams,Jaideep KapurPubMed 36750584
- Animals : an open access journal from MDPI 13:2022Equine Stomach Development in the Foetal Period of Prenatal Life-An Immunohistochemical Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominik Poradowski,Aleksander ChrószczPubMed 36611768
- Cell reports 41:1117192022Glycemic control releases regenerative potential of pancreatic beta cells blocked by severe hyperglycemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJudith Furth-Lavi,Ayat Hija,Sharona Tornovsky-Babeay,Adi Mazouz,Tehila Dahan,Miri Stolovich-Rain,Agnes Klochendler,Yuval Dor,Dana Avrahami,Benjamin GlaserPubMed 36450253
- The Journal of physiology 600:2973-29992022Epidural electrical stimulation of the cervical spinal cord opposes opioid-induced respiratory depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuyi Huang,Jason Worrell,Eric Garner,Stephanie Wang,Tali Homsey,Bo Xu,Erika L Galer,Yan Zhou,Sherwin Tavakol,Meelod Daneshvar,Timothy Le,Harry V Vinters,Noriko Salamon,David L McArthur,Marc R Nuwer,Irene Wu,James C Leiter,Daniel C LuPubMed 35639046
- Oncogenesis 10:832021Cdk5 drives formation of heterogeneous pancreatic neuroendocrine tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela M Carter,Nilesh Kumar,Brendon Herring,Chunfeng Tan,Rachael Guenter,Rahul Telange,Wayne Howse,Fabrice Viol,Tyler R McCaw,Hayden H Bickerton,Priyanka Gupta,Frank Gillardon,Eugene A Woltering,Deepti Dhall,John Totenhagen,Ronadip R Banerjee,Elizabeth M Kurian,Sushanth Reddy,Herbert Chen,Joerg Schrader,J Bart Rose,M Shahid Mukhtar,James A BibbPubMed 34862365
- Cellular and molecular gastroenterology and hepatology 13:309-337.e32021Unique Neural Circuit Connectivity of Mouse Proximal, Middle, and Distal Colon Defines Regional Colonic Motor Patterns.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Nestor-Kalinoski,Kristen M Smith-Edwards,Kimberly Meerschaert,Joseph F Margiotta,Bartek Rajwa,Brian M Davis,Marthe J HowardPubMed 34509687
- Molecular metabolism 53:1012682021K channel blockers control glucagon secretion by distinct mechanisms: A direct stimulation of α-cells involving a [Ca] rise and an indirect inhibition mediated by somatostatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBilal Singh,Firas Khattab,Heeyoung Chae,Lieven Desmet,Pedro L Herrera,Patrick GilonPubMed 34118477
- Scientific reports 10:215162020The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyako Y Murayama et. al.PubMed 33299078
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Tested | Not recommended |
| Chimpanzee | Predicted | Not recommended |
| Cow | Predicted | Not recommended |
| Dog | Predicted | Not recommended |
| Gorilla | Predicted | Not recommended |
| Horse | Predicted | Not recommended |
| Orangutan | Predicted | Not recommended |
| Pig | Predicted | Not recommended |
| Rabbit | Predicted | Not recommended |
| Sheep | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Somatostatin-14Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin.NeuronostatinMay enhance low-glucose-induced glucagon release by pancreatic alpha cells (By similarity). This effect may be mediated by binding to GPR107 and PKA activation (By similarity). May regulate cardiac contractile function (By similarity). May compromise cardiomyocyte viability (By similarity). In the central nervous system, may impair memory retention and may affect hippocampal excitability (By similarity). May also have anxiolytic and anorexigenic effects (By similarity). May play a role in arterial pressure regulation (By similarity). May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH (PubMed:29615476).
Alternative names
Somatostatin, Growth hormone release-inhibiting factor, SST
Recommended products
Anti-Somatostatin antibody ab108456 is a rabbit polyclonal antibody that is used in Somatostatin IHC. Suitable for human, mouse and rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Somatostatin also known as growth hormone-inhibiting hormone (GHIH) is a peptide hormone with a molecular mass around 3200 Daltons. This protein is expressed mainly in the central nervous system including the hypothalamus as well as in the pancreas and gastrointestinal tract. Somatostatin consists of two biologically active forms: one with 14 amino acids and another with 28 amino acids. This target exerts its mechanical function by binding to specific G-protein-coupled somatostatin receptors leading to inhibition of various signaling pathways.
- Biological function summary
Somatostatin regulates the endocrine system and affects neurotransmission and cell proliferation. It inhibits the secretion of several hormones such as growth hormone thyroid-stimulating hormone and insulin. Somatostatin also plays a role in reducing gastric acid secretion and slowing down gastrointestinal motility. While somatostatin does not act as part of a larger complex its action is highly localized in the tissues where it is produced and released.
- Pathways
Somatostatin acts significantly in both the growth hormone and insulin signaling pathways. In the growth hormone pathway it serves as a counter-regulatory hormone to growth hormone-releasing hormone (GHRH) therefore controlling growth hormone levels. In the insulin signaling pathway somatostatin interacts with insulin and glucagon modulating glucose metabolism. It is also related to other proteins like cortistatin which shares similar functions and can bind to the same receptors.
- Associated diseases and disorders
Somatostatin is linked with conditions such as acromegaly and diabetes. In acromegaly excessive growth hormone production occurs due to reduced somatostatin activity making somatostatin analogs a therapeutic option. Meanwhile in diabetes altered somatostatin levels can influence insulin and glucagon balance affecting glucose homeostasis. Somatostatin’s interaction with its receptors and proteins like insulin plays an important role in the management of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody (ab108456)
IHC image of Somatostatin staining in a section of formalin-fixed paraffin-embedded normal rat pancreas performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab108456, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody (ab108456)
IHC image of Somatostatin staining in a section of formalin-fixed paraffin-embedded normal mouse pancreas performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab108456, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody (ab108456)
IHC image of Somatostatin staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108456, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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