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AB326063

Anti-Somatostatin antibody [EPR30824-36] - BSA and Azide free

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Rabbit Recombinant Monoclonal Somatostatin antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.

View Alternative Names

Somatostatin, Growth hormone release-inhibiting factor, SST

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Competitive ELISA - Anti-Somatostatin antibody [EPR30824-36] - BSA and Azide free (AB326063)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Somatostatin antibody [EPR30824-36] - BSA and Azide free (AB326063)

ab326063 (0.05 µg/mL) is specific to Somatostatin and the percent cross reactivity with other compounds is demonstrated in the table.

Competitive ELISA - Anti-Somatostatin antibody [EPR30824-36] - BSA and Azide free (AB326063)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Somatostatin antibody [EPR30824-36] - BSA and Azide free (AB326063)

0.2 µg/mL Somatostatin-crosslink-BSA was coated onto 96-wells. Serial dilution of free Somatostatin (0, 0.195, 0.391, 0.781, 1.563, 3.125, 6.25, 12.5, 25, 50 ng/mL) was added (50 µL). This was followed by adding 0.05 µg/mL ab326063 (50 µL) into each well. The plate was incubated for 30 mins. After washing, 1 : 20000 diluted GAR Fc-HRP was added and incubated for 30 mins. After washing, 100 µL of TMB was added and incubated for 5-10 mins without shaking for color development. Then 100 µL of stop solution (4.9% Orthophosphoric acid) was dispensed and OD was read at 450nm on a microplate reader within 5-10 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30824-36

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody shows 100% cross-reactivity with Somatostatin-28 and shows no cross-reactivity with Cortistatin-17. Working range: 0.2-50 ng/mL.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab326063 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for competitive ELISAs to quantify Somatostatin.

The reference range value is 0.2-50 ng/mL.

Please note that the range provided for this antibody is only an estimation. Performance of the antibody depends on the specific characteristics of your assay. We guarantee the product works in competitive ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Somatostatin-14. Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin.. Neuronostatin. May enhance low-glucose-induced glucagon release by pancreatic alpha cells (By similarity). This effect may be mediated by binding to GPR107 and PKA activation (By similarity). May regulate cardiac contractile function (By similarity). May compromise cardiomyocyte viability (By similarity). In the central nervous system, may impair memory retention and may affect hippocampal excitability (By similarity). May also have anxiolytic and anorexigenic effects (By similarity). May play a role in arterial pressure regulation (By similarity). May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH (PubMed : 29615476).
See full target information Somatostatin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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