AB216649

Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal Somatostatin Receptor 2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Cited in 13 publications.

View Alternative Names

Somatostatin receptor type 2, SS-2-R, SS2-R, SS2R, SST2, SRIF-1, SSTR2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab134152).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab134152).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Somatostatin Receptor 2 with ab134152 at 1/2000 dilution (0.928 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with ab134152 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas labelling somatostatin receptor 2 with ab134152 at a concentration of 0.2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab134152 anti somatostatin receptor 2 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152). Tissue Microarrays stained for Anti-Somatostatin Receptor 2 antibody [UMB1] using ab134152 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested.The sections were incubated with ab134152 for 30 mins at room temperature used at 1 : 2000 dilution (0.928 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Counterstain was Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Unconjugated

Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
Carrier free

Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Somatostatin Receptor 2 antibody [UMB1]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

UMB1

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody was demonstrated in a Somatostatin Receptor 2-deficient mouse model. Refer to Fischer et al.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "PubMed: 23630299" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab216649 is the carrier-free version of ab134152.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The somatostatin receptor 2 (SSTR2) also known as SS2R serves as a G protein-coupled receptor with a molecular mass of approximately 40-60 kDa. SSTR2 specifically binds somatostatin a peptide hormone that regulates the endocrine system neurotransmission and cell proliferation via interaction with various somatostatin receptors. Scientists find SSTR2 expressed in high levels in the pituitary gland central nervous system gastrointestinal tract and pancreatic cells.

Biological function summary

Somatostatin receptor 2 mediates the inhibitory effects of somatostatin on cell growth and the release of other hormones. It functions as part of the receptor complex modulating intracellular signaling pathways. SSTR2 often interacts closely with other molecules like G proteins to inhibit adenylyl cyclase reducing cyclic AMP (cAMP) levels and influencing diverse cellular responses. The receptor's ability to influence such processes emphasizes its role in maintaining cellular and systemic homeostasis.

Pathways

Somatostatin receptor 2 plays an important role in the inhibitory signaling pathways related to growth hormone regulation and neurotransmitter release. SSTR2 actively participates in the somatostatin signaling pathway intimately interacting with proteins such as adenylate cyclase and phospholipase C. Another important pathway includes the regulation of the MAPK pathway serving to modulate cell proliferation and survival reiterating its regulatory function within endocrine signaling and cellular communication.

Somatostatin receptor 2 demonstrates significant involvement in neuroendocrine tumors and acromegaly. The overexpression of SSTR2 in neuroendocrine tumors provides a valuable target for diagnostic imaging and therapeutic interventions using somatostatin analogs. In acromegaly where excess growth hormone production occurs SSTR2's modulation of growth hormone release becomes important; it often works alongside growth hormone receptors and IGF-1 to limit abnormal growth. Therefore the receptor's targeting offers therapeutic potential and advances disease understanding.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.

See full target information SSTR2

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 130:441-3 PubMed26195322

2015

Somatostatin receptor 2a is a more sensitive diagnostic marker of meningioma than epithelial membrane antigen.

Applications

IHC, IHC-P

Species

Human, Human

Joshua R Menke,David R Raleigh,Allen M Gown,Sean Thomas,Arie Perry,Tarik Tihan

Gland surgery 3:E1-5 PubMed25493261

2014

Detection of insulinoma using (68)Gallium-DOTATATE PET/CT: a case report.

Applications

IHC

Species

Human

Samira M Sadowski,Vladimir Neychev,Candice Cottle-Delisle,Roxanne Merkel,Lily A Yang,Martha M Quezado,Richard Chang,Electron Kebebew

PloS one 9:e91526 PubMed24637622

2014

Carboxyl-terminal receptor domains control the differential dephosphorylation of somatostatin receptors by protein phosphatase 1 isoforms.

Applications

ICC/IF

Species

Human

Andreas Lehmann,Andrea Kliewer,Jan Carlo Märtens,Falko Nagel,Stefan Schulz

Trends in pharmacological sciences 34:676-88 PubMed24183675

2013

Illuminating somatostatin analog action at neuroendocrine tumor receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Claude Reubi,Agnes Schonbrunn

Cancer research 73:6865-73 PubMed24080280

2013

Free somatostatin receptor fraction predicts the antiproliferative effect of octreotide in a neuroendocrine tumor model: implications for dose optimization.

Applications

Unspecified application

Species

Unspecified reactive species

Pedram Heidari,Eric Wehrenberg-Klee,Peiman Habibollahi,Daniel Yokell,Matthew Kulke,Umar Mahmood

Human pathology 44:2711-8 PubMed24060005

2013

Phosphaturic mesenchymal tumors show positive staining for somatostatin receptor 2A (SSTR2A).

Applications

IHC

Species

Human

Michelle Houang,Adele Clarkson,Loretta Sioson,Marianne S Elston,Roderick J Clifton-Bligh,Michael Dray,Dominique Ranchere-Vince,Anne-Valerie Decouvelaere,Arnaud de la Fouchardiere,Anthony J Gill

Diabetes 62:2968-77 PubMed23630299

2013

Somatostatin receptor type 2 antagonism improves glucagon counterregulation in biobreeding diabetic rats.

Applications

IHC-Fr

Species

Rat

Negar Karimian,Tairan Qin,Tao Liang,Mayowa Osundiji,Yachi Huang,Trevor Teich,Michael C Riddell,Mark S Cattral,David H Coy,Mladen Vranic,Herbert Y Gaisano

The Journal of clinical endocrinology and metaboli 98:687-94 PubMed23295468

2013

Improving diagnosis of tumor-induced osteomalacia with Gallium-68 DOTATATE PET/CT.

Applications

IHC-P

Species

Human

Roderick J Clifton-Bligh,Michael S Hofman,Emma Duncan,Ie-Wen Sim,David Darnell,Adele Clarkson,Tricia Wong,John P Walsh,Anthony J Gill,Peter R Ebeling,Rodney J Hicks

Gene therapy 20:283-97 PubMed22592599

2012

Sstr2A: a relevant target for the delivery of genes into human glioblastoma cells using fiber-modified adenoviral vectors.

Applications

Unspecified application

Species

Unspecified reactive species

K Lécolle,S Bégard,R Caillierez,D Demeyer,E Grellier,A Loyens,Z Csaba,J C Beauvillain,J C D'Halluin,M Baroncini,J P Lejeune,A Sharif,V Prévot,P Dournaud,L Buée,M Colin

International journal of clinical and experimental 5:187-94 PubMed22558472

2012

Comparing of IRS and Her2 as immunohistochemical scoring schemes in gastroenteropancreatic neuroendocrine tumors.

Applications

IHC-P

Species

Human

Daniel Kaemmerer,Luisa Peter,Amelie Lupp,Stefan Schulz,Jörg Sänger,Richard Paul Baum,Vikas Prasad,Merten Hommann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (AB216649)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

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Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise