Anti-Somatostatin Receptor 5 antibody [UMB4]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (AB109495)

Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas labelling Somatostatin Receptor 5 with ab109495 at a concentration of 8 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. The heat-mediated antigen retrieval was conducted with DISCOVERY cell conditioning solution (CC1) at 100 °C, pH 8.5 for 32 mins. ab109495 Anti-Somatostatin Receptor 5 antibody [UMB4] was incubated for 16 mins, at 37 °C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (AB109495)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Somatostatin Receptor 5 with Purified ab109495 at 1 : 50 dilution (16.3 µg/ml). Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.. Performed on a Leica Biosystems BOND ® RX instrument.was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (AB109495)

Somatostatin receptor 2 (SSTR2), somatostatin receptor 5 (SSTR5) and CXCR4 expression in the hepatoblastoma cell line HepG2 and in the hepatoma cell lines Hep3B and HuH-7 in comparison to the small cell lung cancer cell line NCI-H69 and the neuroendocrine tumor cell line BON-1. Immunohistochemistry (red-brown color), counterstaining with hematoxylin; scale bar : 20 μm. Representative photomicrographs of three independent batches are shown.

HepG2, Hep3B, HuH-7, NCI-H-69, and BON-1 cells (DSMZ, Braunschweig, Germany) were grown in 75 cm² culture flasks to a confluency of 80%. Cells were washed once with phosphate-buffered saline and transferred into 10% buffered formalin (J.T.Baker, Deventer, The Netherlands) for 2 h. After centrifugation for 10 min at 3500 x g, the supernatant was removed, and 1 ml human pool plasma was added to the cell samples. After brief vortexing, 100 μl human fibrinogen (50–70% protein; ≥80% clottable) was added to each sample, and the samples were vortexed again. The resulting clots were placed for another 24 h in 10% buffered formalin and embedded in paraffin blocks.

Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• WB

Unknown

Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (AB109495)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 0.08 µg/mL

All lanes:

293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false