AB121759

Anti-SON/DBP5 antibody

Name: Anti-SON/DBP5 antibody (ab121759) | Abcam
Brand: Abcam Limited
SKU: AB121759
Rating: 5 (1 reviews)

(1 Review)

(14 Publications)

Anti-SON/DBP5 antibody (ab121759) is a rabbit polyclonal antibody detecting SON/DBP5 in IHC-P. Suitable for Human.

View Alternative Names

C21orf50, DBP5, KIAA1019, NREBP, HSPC310, HSPC312, SON, Protein SON, Bax antagonist selected in saccharomyces 1, Negative regulatory element-binding protein, Protein DBP-5, SON3, BASS1, NRE-binding protein

5 Images

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Immunocytochemistry - Anti-SON/DBP5 antibody (AB121759)

ab121759, at 4 μg/ml, staining SON/DBP5 (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Paraffin-embedded human prostate tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Paraffin-embedded human small intestine tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong postiivty in nuclear speckles in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Paraffin-embedded human tonsil tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in germinal center cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Paraffin-embedded human cerebellum tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in Purkinje cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SON aa 1450-1600. The exact immunogen used to generate this antibody is proprietary information.

P18583

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-SON/DBP5 antibody (ab121759) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence : 5'-GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Essential for correct RNA splicing of multiple genes critical for brain development, neuronal migration and metabolism, including TUBG1, FLNA, PNKP, WDR62, PSMD3, PCK2, PFKL, IDH2, and ACY1 (PubMed : 27545680).

See full target information SON

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40900123

2025

DNA methylation insulates genic regions from CTCF loops near nuclear speckles.

Applications

Unspecified application

Species

Unspecified reactive species

Shelby A Roseman,Allison P Siegenfeld,Ceejay Lee,Nicholas Z Lue,Amanda L Waterbury,Brian B Liau

Nature communications 16:390 PubMed39755675

2025

Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.

Applications

Unspecified application

Species

Unspecified reactive species

Alexa B R McIntyre,Adrian Beat Tschan,Katrina Meyer,Severin Walser,Arpan Kumar Rai,Keisuke Fujita,Lucas Pelkmans

Nature cell biology 27:322-335 PubMed39747580

2025

Nuclear speckles regulate functional programs in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine A Alexander,Ruofan Yu,Nicolas Skuli,Nathan J Coffey,Son Nguyen,Christine L Faunce,Hua Huang,Ian P Dardani,Austin L Good,Joan Lim,Catherine Y Li,Nicholas Biddle,Eric F Joyce,Arjun Raj,Daniel Lee,Brian Keith,M Celeste Simon,Shelley L Berger

Communications biology 7:763 PubMed38914653

2024

Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Teng Hsiao,I-Hsin Liao,Bo-Kuan Wu,Hsueh-Ping Catherine Chu,Chia-Lung Hsieh

Journal of virology 98:e0190123 PubMed38629840

2024

Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juliette Bougon,Eileigh Kadijk,Lucie Gallot-Lavallee,Bruce A Curtis,Matthew Landers,John M Archibald,Denys A Khaperskyy

Cell stem cell 30:1658-1673.e10 PubMed38065069

2023

SON is an essential mA target for hematopoietic stem cell fate.

Applications

Unspecified application

Species

Unspecified reactive species

Hanzhi Luo,Mariela Cortés-López,Cyrus L Tam,Michael Xiao,Isaac Wakiro,Karen L Chu,Aspen Pierson,Mandy Chan,Kathryn Chang,Xuejing Yang,Daniel Fecko,Grace Han,Eun-Young Erin Ahn,Quaid D Morris,Dan A Landau,Michael G Kharas

Frontiers in cell and developmental biology 11:1217637 PubMed37484912

2023

Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Hoboth,Martin Sztacho,Alexander Quaas,Baki Akgül,Pavel Hozák

PloS one 17:e0274091 PubMed36201559

2022

Epithelial cell size dysregulation in human lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Clifford W Sandlin,Song Gu,Jun Xu,Charuhas Deshpande,Michael D Feldman,Matthew C Good

MethodsX 8:101372 PubMed34430268

2021

Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Hoboth,Ondřej Šebesta,Martin Sztacho,Enrique Castano,Pavel Hozák

Molecular cell 81:1666-1681.e6 PubMed33823140

2021

p53 mediates target gene association with nuclear speckles for amplified RNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine A Alexander,Allison Coté,Son C Nguyen,Liguo Zhang,Omid Gholamalamdari,Paula Agudelo-Garcia,Enrique Lin-Shiao,K M A Tanim,Joan Lim,Nicolas Biddle,Margaret C Dunagin,Charly R Good,Mariel R Mendoza,Shawn C Little,Andrew Belmont,Eric F Joyce,Arjun Raj,Shelley L Berger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SON/DBP5 antibody

Immunocytochemistry - Anti-SON/DBP5 antibody (AB121759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (AB121759)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise