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Rabbit Recombinant Monoclonal SON/DBP5 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (AB317311), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] (AB317311), expandable thumbnail
  • Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (AB317311), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] (AB317311), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (AB317311), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IFIHC-PIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Essential for correct RNA splicing of multiple genes critical for brain development, neuronal migration and metabolism, including TUBG1, FLNA, PNKP, WDR62, PSMD3, PCK2, PFKL, IDH2, and ACY1 (PubMed:27545680).

Alternative names

C21orf50, DBP5, KIAA1019, NREBP, HSPC310, HSPC312, SON, Protein SON, Bax antagonist selected in saccharomyces 1, Negative regulatory element-binding protein, Protein DBP-5, SON3, BASS1, NRE-binding protein

Recommended products

Rabbit Recombinant Monoclonal SON/DBP5 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28617-69
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311), expandable thumbnail

    Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)

    SON/DBP5 Western blot staining using rabbit Anti-SON/DBP5 antibody

    SON can undergo degradation easily.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:18952841, 27545680).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg with NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting SON whole cell lysate at 40 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 264 kDa, 124 kDa

    Exposure time: 26s

  • Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling SON/DBP5 with ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing nuclear staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311), expandable thumbnail

    Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)

    SON can undergo degradation easily.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:18952841, 27545680).

    Lysates of lanes 2-4 were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: A431 whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 264 kDa

    Exposure time: 37s

  • Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431(human epidermoid carcinoma epithelial cell) cells labelling SON/DBP5 with ab317311 at 1/500 dilution (0.1ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SON/DBP5 with ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon carcinoma. The section was incubated with ab317311 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SON/DBP5 with ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human testis. The section was incubated with ab317311 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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