Rabbit Recombinant Monoclonal SON/DBP5 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, WB, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--western-blot-img430067.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312)")
![Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--immunocytochemistry-immunofluorescence-img430093.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312)")
![Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--western-blot-img430068.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312)")
![Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--flow-cytometry-intracellular-img430186.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430110.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (AB317312)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IHC-P | WB | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Essential for correct RNA splicing of multiple genes critical for brain development, neuronal migration and metabolism, including TUBG1, FLNA, PNKP, WDR62, PSMD3, PCK2, PFKL, IDH2, and ACY1 (PubMed:27545680).
Alternative names
C21orf50, DBP5, KIAA1019, NREBP, HSPC310, HSPC312, SON, Protein SON, Bax antagonist selected in saccharomyces 1, Negative regulatory element-binding protein, Protein DBP-5, SON3, BASS1, NRE-binding protein
Recommended products
Rabbit Recombinant Monoclonal SON/DBP5 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28617-69
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317312 is the carrier-free version of Anti-SON/DBP5 antibody [EPR28617-69] ab317311.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--western-blot-img430067.jpg "Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)
SON/DBP5 Western blot staining using rabbit Anti-SON/DBP5 antibody
This data was developed using Anti-SON/DBP5 antibody [EPR28617-69] ab317311, the same antibody clone in a different buffer formulation.
SON can undergo degradation easily.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:18952841, 27545680).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (Anti-SON/DBP5 antibody [EPR28617-69] ab317311) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg with NFDM/TBST
Lane 2: HeLa transfected with siRNA specifically targeting SON whole cell lysate at 40 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 264 kDa, 124 kDa
Exposure time: 26s
![Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--immunocytochemistry-immunofluorescence-img430093.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)
This data was developed using Anti-SON/DBP5 antibody [EPR28617-69] ab317311, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling SON/DBP5 with Anti-SON/DBP5 antibody [EPR28617-69] ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--western-blot-img430068.jpg "Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Western blot - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)
This data was developed using Anti-SON/DBP5 antibody [EPR28617-69] ab317311, the same antibody clone in a different buffer formulation.
SON can undergo degradation easily.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:18952841, 27545680).
Lysates of lanes 2-4 were freshly made and used for Western Blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (Anti-SON/DBP5 antibody [EPR28617-69] ab317311) at 1/1000 dilution
Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: A431 whole cell lysate at 20 µg with NFDM/TBST
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 264 kDa
Exposure time: 37s
![Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--flow-cytometry-intracellular-img430186.jpg "Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)
This data was developed using Anti-SON/DBP5 antibody [EPR28617-69] ab317311, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431(human epidermoid carcinoma epithelial cell) cells labelling SON/DBP5 with Anti-SON/DBP5 antibody [EPR28617-69] ab317311 at 1/500 dilution (0.1ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430110.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)
This data was developed using Anti-SON/DBP5 antibody [EPR28617-69] ab317311, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SON/DBP5 with Anti-SON/DBP5 antibody [EPR28617-69] ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon carcinoma. The section was incubated with Anti-SON/DBP5 antibody [EPR28617-69] ab317311 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430109.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)
This data was developed using Anti-SON/DBP5 antibody [EPR28617-69] ab317311, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SON/DBP5 with Anti-SON/DBP5 antibody [EPR28617-69] ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human testis. The section was incubated with Anti-SON/DBP5 antibody [EPR28617-69] ab317311 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312), expandable thumbnail](https://content.abcam.com/products/images/son-dbp5-antibody-epr28617-69-bsa-and-azide-free-ab317312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430109.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] - BSA and Azide free (ab317312)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com