AB135240

Anti-Sonic Hedgehog antibody [5H4]

Name: Anti-Sonic Hedgehog antibody [5H4] (ab135240) | Abcam
Brand: Abcam Limited
SKU: AB135240

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(13 Publications)

Mouse Monoclonal Sonic Hedgehog antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, African green monkey samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHH aa 1-200.

View Alternative Names

Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC

5 Images

Flow Cytometry - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Flow Cyt

Unknown

Flow Cytometry - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Flow cytometric analysis of HeLa cells labelling Sonic Hedgehog with ab135240 at 1/200 dilution (green). Negative control (red).

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Western blot - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Expected MW is 41 kDa.

All lanes:

Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

All lanes:

Human Sonic Hedgehog recombinant protein

Predicted band size: 49 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Immunohistochemical analysis of Paraffin-embedded Human liver cancer tissue labelling Sonic Hedgehog with ab135240 at 1/200 dilution followed by DAB staining.

Unknown

Western blot - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

All lanes:

Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

HEK293 cell lysate, transfected with Sonic Hedgehog (amino acids 26-161)-hIgGFc

Predicted band size: 119 kDa,16 kDa,17 kDa,33 kDa,35 kDa,44 kDa,49 kDa,61 kDa,62 kDa,65 kDa

false

Unknown

Western blot - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

All lanes:

Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

Lane 1:

LNCaP cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

PANC1 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

SK-N-SH cell lysate

Lane 6:

F9 cell lysate

Lane 7:

NIH 3T3 cell lysate

Lane 8:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5H4

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human, African green monkey

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SHH aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q15465

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR164113-18 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.

Biological function summary

Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.

Pathways

Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.

Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).. Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed : 24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed : 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed : 10753901).

See full target information SHH

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Balkan medical journal 42:440-451 PubMed40888349

2025

Inhibition of PI3K and Hedgehog Signaling Pathway Inhibits Hypoxia-Induced Vasculogenic Mimicry Formation in Ovarian Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liang,Yun Bai,Huan Zhao

Journal of neuroinflammation 21:16 PubMed38200558

2024

Chorioamnionitis accelerates granule cell and oligodendrocyte maturation in the cerebellum of preterm nonhuman primates.

Applications

Unspecified application

Species

Unspecified reactive species

Josef Newman,Xiaoying Tong,April Tan,Toni Yeasky,Vanessa Nunes De Paiva,Pietro Presicce,Paranthaman S Kannan,Kevin Williams,Andreas Damianos,Marione Tamase Newsam,Merline K Benny,Shu Wu,Karen C Young,Lisa A Miller,Suhas G Kallapur,Claire A Chougnet,Alan H Jobe,Roberta Brambilla,Augusto F Schmidt

International journal of molecular sciences 24: PubMed37240278

2023

Expression Pattern of Sonic Hedgehog, Patched and Smoothened in Clear Cell Renal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Dunatov Huljev,Nela Kelam,Benjamin Benzon,Violeta Šoljić,Natalija Filipović,Valdi Pešutić Pisac,Merica Glavina Durdov,Katarina Vukojević

Journal of ovarian research 15:141 PubMed36581994

2022

GIMAP7 induces oxidative stress and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inhibiting sonic hedgehog signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Anran Xu,Yuanyuan Fan,Song Liu,Lianbing Sheng,Yanyan Sun,Huijun Yang

Journal of biophotonics 15:e202200103 PubMed36054290

2022

Enhanced hair growth effects through low-level vortex beams radiation: An experimental animal study.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Zhou,Ziling Huang,Zhenyu Xu,Yue Yin,Yancong He,Lingxiao Huang,Linmao Dai,Xiaochun Li,Siqi Fu,Huihui Huang

Cellular oncology (Dordrecht) 45:639-658 PubMed35902550

2022

Selective targeting BMP2 and 4 in SMAD4 negative esophageal adenocarcinoma inhibits tumor growth and aggressiveness in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Shulin Li,Sanne J M Hoefnagel,Matthew Read,Sybren Meijer,Mark I van Berge Henegouwen,Suzanne S Gisbertz,Elena Bonora,David S H Liu,Wayne A Phillips,Silvia Calpe,Ana C P Correia,Maria D C Sancho-Serra,Sandro Mattioli,Kausilia K Krishnadath

Blood advances 6:5024-5040 PubMed35704688

2022

Noncanonical Sonic Hedgehog signaling amplifies platelet reactivity and thrombogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Arundhati Tiwari,Deepa Gautam,Paresh P Kulkarni,Mohammad Ekhlak,Vijay K Sonkar,Vikas Agrawal,Debabrata Dash

Respiratory research 23:86 PubMed35395850

2022

C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yang,Wei Sun,Xiaoyan Jing,Qian Zhang,Hui Huang,Zuojun Xu

Oxidative medicine and cellular longevity 2022:9955677 PubMed35265269

2022

Exogenous Parathyroid Hormone Alleviates Intervertebral Disc Degeneration through the Sonic Hedgehog Signalling Pathway Mediated by CREB.

Applications

Unspecified application

Species

Unspecified reactive species

You Li,Yifan Wei,He Li,Hui Che,Dengshun Miao,Cheng Ma,Yongxin Ren

International journal of biological sciences 18:956-969 PubMed35173529

2022

A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wu,Rong Wang,Xuechun Kan,Jinghan Zhang,Wen Sun,David Goltzman,Dengshun Miao

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Sonic Hedgehog antibody [5H4]

Flow Cytometry - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Western blot - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Western blot - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Western blot - Anti-Sonic Hedgehog antibody [5H4] (AB135240)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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