Rabbit Polyclonal Sonic Hedgehog antibody. Suitable for WB, sELISA and reacts with Mouse, Synthetic peptide - Human samples. Cited in 24 publications.
Images
Publications
Filter by
- Journal of biophotonics 16:e2023000432023Regeneration of taste through sonic hedgehog upregulation by photobiomodulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHa Young Lee,Celine Dg Abueva,Andrew Padalhin,So Young Park,Hyun Seok Ryu,Phil-Sang Chung,Hee Jung Kim,Jeongyun Kim,Seung Hoon WooPubMed 37483112
- Neural regeneration research 18:2208-22182023M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Gui Huang,Jiang-Xia Ren,Yue Chen,Ming-Fen Tian,Li Zhou,Jun Wen,Xiao-Song Song,You-Lin Wu,Qing-Huan Yang,Pei-Ran Jiang,Jia-Ni Wang,Qin YangPubMed 37056140
- Molecular biology reports 50:3317-33272023Sirt1 regulates microglial activation and inflammation following oxygen-glucose deprivation/reoxygenation injury by targeting the Shh/Gli-1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Liao,Jiagui Huang,Jie Liu,Huimin Zhu,Yue Chen,Xuemei Li,Jun Wen,Qin YangPubMed 36725745
- Stem cells international 2020:88825492020Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArjun Sinkemani et. al.PubMed 33424982
- Life sciences 262:1184772020CtBP2 interacts with ZBTB18 to promote malignancy of glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Chen et. al.PubMed 32971103
- Aging 12:11446-114652020microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy ISL1-mediated sonic hedgehog signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Sun et. al.PubMed 32544883
- Artificial cells, nanomedicine, and biotechnology 48:939-9472020Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Tian,Zhenkun HePubMed 32496832
- Bioscience reports :2020Regulation of miR-375 and Sonic hedgehog on vascular endothelial growth factor in preeclampsia rats and its effect on trophoblast cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanfang Ren et. al.PubMed 32412057
- Diabetes 69:1549-15612020Role of VIP and Sonic Hedgehog Signaling Pathways in Mediating Epithelial Wound Healing, Sensory Nerve Regeneration, and Their Defects in Diabetic Corneas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangyang Zhang et. al.PubMed 32345752
- International journal of molecular sciences 21:2020Discrete Hedgehog Factor Expression and Action in the Developing Phallus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGerard A Tarulli et. al.PubMed 32059607
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | sELISA | IHC-Fr | |
|---|---|---|---|
| Human | Predicted | Predicted | Not recommended |
| Mouse | Tested | Expected | Not recommended |
| Rat | Predicted | Predicted | Not recommended |
| Chicken | Predicted | Predicted | Not recommended |
| Cow | Predicted | Predicted | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended |
| Xenopus laevis | Predicted | Predicted | Not recommended |
| Zebrafish | Predicted | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Human, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Human, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Did not detect Shh using IHC-Fr of E11.5 and E13.5 embryonic mouse brain sections at 1ug/ml following paraformaldehyde fixation |
Species Rat | Dilution info - | Notes - |
Species Chicken | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Xenopus laevis | Dilution info - | Notes - |
Species Zebrafish | Dilution info - | Notes - |
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity). Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed:24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed:10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed:10753901).
Alternative names
Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
Recommended products
Rabbit Polyclonal Sonic Hedgehog antibody. Suitable for WB, sELISA and reacts with Mouse, Synthetic peptide - Human samples. Cited in 24 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.
- Biological function summary
Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.
- Pathways
Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.
- Associated diseases and disorders
Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Anti-Sonic Hedgehog antibody (ab19897)
Western blot - Anti-Sonic Hedgehog antibody (ab19897)
ab19897 detects a 180 AA (22 kDa) recombinant fragment of mouse Sonic Hedgehog (lane 1). Binding of ab19897 to Sonic Hedgehog was reduced when blocking using the immunising peptide (lane 2).
All lanes: Western blot - Anti-Sonic Hedgehog antibody (ab19897) at 1 µg/mL
Lane 1: 22 kDa fragment of Mouse recombinant Sonic Hedgehog protein at 1 µg
Lane 2: 22 kDa fragment of Mouse recombinant Sonic Hedgehog protein at 1 µg with Human Sonic Hedgehog peptide (ab21448)
Predicted band size: 49 kDa
Observed band size: 22 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com