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AB240438

Anti-Sonic Hedgehog antibody

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(5 Publications)

Goat Polyclonal Sonic Hedgehog antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SHH aa 400 to C-terminus.

View Alternative Names

Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (AB240438)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (AB240438)

Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Sonic Hedgehog (Green) using ab240438 in ICC/IF. Primary incubation for 1 hour (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (Blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody (AB240438)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody (AB240438)

Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) labeling Sonic Hedgehog (blue line) with ab240438. Primary incubation for 1 hour at 10 μg/ml, followed by Alexa Fluor®488 secondary antibody (1 μg/ml).

IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor®488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (AB240438)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (AB240438)

Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized U-251 MG (human brain glioma cell line) cells labeling Sonic Hedgehog (Green) using ab240438 in ICC/IF. Primary incubation for 1 hour (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (AB240438)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (AB240438)

Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Sonic Hedgehog (Green) using ab240438 in ICC/IF. Primary incubation for 1 hour (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (Blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SHH aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15465

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.
Biological function summary

Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.

Pathways

Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.

Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the endoplasmic reticulum (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).. Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed : 24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed : 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed : 10753901).
See full target information SHH
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684590

2024

Gliotic Response and Reprogramming Potential of Human Müller Cell Line MIO-M1 Exposed to High Glucose and Glucose Fluctuations.

Applications

Unspecified application

Species

Unspecified reactive species

Benedetta Russo,Giorgia D'Addato,Giulia Salvatore,Marika Menduni,Simona Frontoni,Luigi Carbone,Antonella Camaioni,Francesca Gioia Klinger,Massimo De Felici,Fabiana Picconi,Gina La Sala

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403627 PubMed39535354

2024

Spatiotemporal Molecular Architecture of Lineage Allocation and Cellular Organization in Tooth Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shengjie Jiang,Yuning Zhang,Huimin Zheng,Kai Zhao,Yue Yang,Binbin Lai,Xuliang Deng,Yan Wei

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2310048 PubMed39474995

2024

Cathepsin K-Positive Cell Lineage Promotes In Situ Dentin Formation Controlled by Nociceptive Sonic Hedgehog.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoshi Xu,Xiaohan Zhang,Weimin Lin,Yushun Wang,Danting Zhang,Shuang Jiang,Linfeng Liu,Jiaying Wang,Xutao Luo,Xiao Zhang,Junjun Jing,Quan Yuan,Chenchen Zhou

Scientific reports 13:15508 PubMed37726363

2023

A 3D adrenocortical carcinoma tumor platform for preclinical modeling of drug response and matrix metalloproteinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priya H Dedhia,Hemamylammal Sivakumar,Marco A Rodriguez,Kylie G Nairon,Joshua M Zent,Xuguang Zheng,Katie Jones,Liudmila V Popova,Jennifer L Leight,Aleksander Skardal

Journal of cellular biochemistry 121:3256-3265 PubMed31904145

2020

Interference with SMO increases chemotherapy drug sensitivity of A2780/DDP cells by inhibiting the Hh/Gli signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shihong Ma,Dan Liu,Wenhua Tan,Botao Du,Wei Liu,Weijia Li,Yufei Jiao
View all publications
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Product promise

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