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AB86462

Anti-Sonic Hedgehog antibody [RM0128-4A37]

4

(1 Review)

|

(2 Publications)

Rat Monoclonal Sonic Hedgehog antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Shh.

View Alternative Names

Hhg1, Shh, Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM0128-4A37

Isotype

IgG2

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Shh. The exact immunogen used to generate this antibody is proprietary information.

Q62226

Specificity

ab86462 detects Sonic Hedgehog, but not dhh or ihh.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab86462 was produced from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
IgG fraction of culture supernatant purified by Protein A/G affinity chromatography and 0.2 µm filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.
Biological function summary

Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.

Pathways

Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.

Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (PubMed : 7736596, PubMed : 7891723, PubMed : 8824192). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (PubMed : 8824192). Both activities occur in the reticulum endoplasmic (PubMed : 21357747). Once cleaved, ShhC is degraded in the endoplasmic reticulum (PubMed : 21357747).. Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed : 11430830, PubMed : 24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (PubMed : 15315762). Essential for axon guidance (PubMed : 12679031). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (By similarity).
See full target information Shh
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 146: PubMed31511252

2019

Developmentally regulated expression is robust to TAD perturbations.

Applications

Unspecified application

Species

Unspecified reactive species

Iain Williamson,Lauren Kane,Paul S Devenney,Ilya M Flyamer,Eve Anderson,Fiona Kilanowski,Robert E Hill,Wendy A Bickmore,Laura A Lettice

Development (Cambridge, England) 141:1715-25 PubMed24715461

2014

Development of five digits is controlled by a bipartite long-range cis-regulator.

Applications

IHC-P

Species

Mouse

Laura A Lettice,Iain Williamson,Paul S Devenney,Fiona Kilanowski,Julia Dorin,Robert E Hill
View all publications
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