AB189248

Anti-Sorbitol Dehydrogenase antibody [EPR15857]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Sorbitol Dehydrogenase antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Sorbitol dehydrogenase, SDH, L-iditol 2-dehydrogenase, Polyol dehydrogenase, Ribitol dehydrogenase, Xylitol dehydrogenase, RDH, XDH, SORD

3 Images

Supplier Data

Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (AB189248)

Immunoprecipitation of Human fetal liver labeling Sorbitol Dehydrogenase with ab189248 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

All lanes:

Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (ab189248)

Predicted band size: 38 kDa

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Supplier Data

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (AB189248)

All lanes:

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (ab189248) at 1/20000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Lane 3:

Hela cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (AB189248)

All lanes:

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (ab189248) at 1/5000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

C6 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Carrier free

Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15857

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sorbitol Dehydrogenase (SDH) also known as SORD is an enzyme that catalyzes the oxidation of sorbitol to fructose. This reaction involves the conversion of a secondary alcohol group to a ketone. SDH has a molecular mass of approximately 38 kDa. It is expressed in various human tissues most importantly in the liver kidneys and seminal vesicles. The enzyme requires NAD+ as a cofactor for its catalytic activity.

Biological function summary

SDH plays a part in the polyol pathway which is important for the metabolism of glucose into fructose. It functions alongside aldose reductase to regulate the conversion of glucose into sorbitol and then sorbitol into fructose. SDH acts independently without forming a complex with other proteins. The amount and activity of SDH can affect cellular osmotic balance and energy storage.

Pathways

SDH is involved in the polyol pathway and influences sorbitol levels. An excess of sorbitol can contribute to cellular damage especially in the context of diabetes. SDH’s role is linked to the exclusive conversion of sorbitol to fructose contrasting with aldose reductase which initiates sorbitol production from glucose. Both enzymes are key in maintaining the balance between extracellular and intracellular glucose.

Alterations in SDH activity relate to diabetic complications like diabetic retinopathy and neuropathy. The accumulation of sorbitol due to insufficient SDH activity contributes to the development of these conditions. SDH's connection with aldose reductase is particularly notable here as both proteins impact the sorbitol levels that affect various tissues under hyperglycemic conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively (PubMed : 3365415). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia (PubMed : 12962626, PubMed : 25105142, PubMed : 29966615). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (PubMed : 16278369). May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor (PubMed : 3365415).

See full target information SORD

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113100 PubMed37676773

2023

Mechanism and evolutionary origins of alanine-tail C-degron recognition by E3 ligases Pirh2 and CRL2-KLHDC10.

Applications

Unspecified application

Species

Unspecified reactive species

Pratik Rajendra Patil,A Maxwell Burroughs,Mohit Misra,Federico Cerullo,Carlos Costas-Insua,Hao-Chih Hung,Ivan Dikic,L Aravind,Claudio A P Joazeiro

NPJ genomic medicine 6:1 PubMed33397963

2021

Biallelic SORD pathogenic variants cause Chinese patients with distal hereditary motor neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Lin Dong,Jia-Qi Li,Gong-Lu Liu,Hao Yu,Zhi-Ying Wu

Nature genetics 52:473-481 PubMed32367058

2020

Biallelic mutations in SORD cause a common and potentially treatable hereditary neuropathy with implications for diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Cortese,Yi Zhu,Adriana P Rebelo,Sara Negri,Steve Courel,Lisa Abreu,Chelsea J Bacon,Yunhong Bai,Dana M Bis-Brewer,Enrico Bugiardini,Elena Buglo,Matt C Danzi,Shawna M E Feely,Alkyoni Athanasiou-Fragkouli,Nourelhoda A Haridy,Rosario Isasi,Alaa Khan,Matilde Laurà,Stefania Magri,Menelaos Pipis,Chiara Pisciotta,Eric Powell,Alexander M Rossor,Paola Saveri,Janet E Sowden,Stefano Tozza,Jana Vandrovcova,Julia Dallman,Elena Grignani,Enrico Marchioni,Steven S Scherer,Beisha Tang,Zhiqiang Lin,Abdullah Al-Ajmi,Rebecca Schüle,Matthis Synofzik,Thierry Maisonobe,Tanya Stojkovic,Michaela Auer-Grumbach,Mohamed A Abdelhamed,Sherifa A Hamed,Ruxu Zhang,Fiore Manganelli,Lucio Santoro,Franco Taroni,Davide Pareyson,Henry Houlden,David N Herrmann,Mary M Reilly,Michael E Shy,R Grace Zhai,Stephan Zuchner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sorbitol Dehydrogenase antibody [EPR15857]

Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (AB189248)

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (AB189248)

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (AB189248)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise