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Rabbit Recombinant Monoclonal Sorbitol Dehydrogenase antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (AB250982), expandable thumbnail
  • Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (AB250982), expandable thumbnail
  • Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (AB250982), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively (PubMed:3365415). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia (PubMed:12962626, PubMed:25105142, PubMed:29966615). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (PubMed:16278369). May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor (PubMed:3365415).

Alternative names

Sorbitol dehydrogenase, SDH, L-iditol 2-dehydrogenase, Polyol dehydrogenase, Ribitol dehydrogenase, Xylitol dehydrogenase, RDH, XDH, SORD

Recommended products

Rabbit Recombinant Monoclonal Sorbitol Dehydrogenase antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15857
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250982 is the carrier-free version of Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sorbitol Dehydrogenase (SDH) also known as SORD is an enzyme that catalyzes the oxidation of sorbitol to fructose. This reaction involves the conversion of a secondary alcohol group to a ketone. SDH has a molecular mass of approximately 38 kDa. It is expressed in various human tissues most importantly in the liver kidneys and seminal vesicles. The enzyme requires NAD+ as a cofactor for its catalytic activity.

Biological function summary

SDH plays a part in the polyol pathway which is important for the metabolism of glucose into fructose. It functions alongside aldose reductase to regulate the conversion of glucose into sorbitol and then sorbitol into fructose. SDH acts independently without forming a complex with other proteins. The amount and activity of SDH can affect cellular osmotic balance and energy storage.

Pathways

SDH is involved in the polyol pathway and influences sorbitol levels. An excess of sorbitol can contribute to cellular damage especially in the context of diabetes. SDH’s role is linked to the exclusive conversion of sorbitol to fructose contrasting with aldose reductase which initiates sorbitol production from glucose. Both enzymes are key in maintaining the balance between extracellular and intracellular glucose.

Associated diseases and disorders

Alterations in SDH activity relate to diabetic complications like diabetic retinopathy and neuropathy. The accumulation of sorbitol due to insufficient SDH activity contributes to the development of these conditions. SDH's connection with aldose reductase is particularly notable here as both proteins impact the sorbitol levels that affect various tissues under hyperglycemic conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (ab250982), expandable thumbnail

    Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (ab250982)

    This data was developed using Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248) at 1/20000 dilution

    Lane 1: Human fetal kidney tissue lysate at 20 µg

    Lane 2: Human fetal liver tissue lysate at 20 µg

    Lane 3: Hela cell lysate at 20 µg

    Lane 4: PC-3 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (ab250982), expandable thumbnail

    Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (ab250982)

    This data was developed using Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248, the same antibody clone in a different buffer formulation.Immunoprecipitation of Human fetal liver labeling Sorbitol Dehydrogenase with Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

    All lanes: Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248)

    Predicted band size: 38 kDa

  • Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (ab250982), expandable thumbnail

    Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] - BSA and Azide free (ab250982)

    This data was developed using Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Sorbitol Dehydrogenase antibody [EPR15857] (Anti-Sorbitol Dehydrogenase antibody [EPR15857] ab189248) at 1/5000 dilution

    Lane 1: Mouse kidney tissue lysate at 10 µg

    Lane 2: Mouse spleen tissue lysate at 10 µg

    Lane 3: Rat kidney tissue lysate at 10 µg

    Lane 4: C6 lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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