Anti-SorCS1 antibody [EPR30536-522]

• WB

Lab

Western blot - Anti-SorCS1 antibody [EPR30536-522] (AB325598)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human SORCS3 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (130KDa).

All lanes:

Western blot - Anti-SorCS1 antibody [EPR30536-522] (ab325598) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human SORCS1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a human SORCS3 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-SorCS1 antibody [EPR30536-522] (AB325598)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : WI-38

Samples are non-boiled as boiling may cause protein aggregation.

Lanes 2-3 developed were using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Lane1 was incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 2-3 were incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-SorCS1 antibody [EPR30536-522] (ab325598) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

WI-38 (human fetal lung fibroblast) whole cell lysate at 40 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 2 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-SorCS1 antibody [EPR30536-522] (AB325598)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen(PMID : 12482870)

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-SorCS1 antibody [EPR30536-522] (ab325598) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 40 µg

Lane 2:

Mouse E14 brain lysate at 40 µg

Lane 3:

Mouse spleen tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-SorCS1 antibody [EPR30536-522] (AB325598)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen(PMID : 12482870)

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-SorCS1 antibody [EPR30536-522] (ab325598) at 1/1000 dilution

Lane 1:

Rat brainstem tissue lysate at 40 µg

Lane 2:

Rat E15 brain lysate at 40 µg

Lane 3:

Rat spleen tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

true

Exposure time: 180s