Skip to main content

Rabbit Recombinant Monoclonal SorLA/SORL1 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (AB302508), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (AB302508), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (AB302508), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (AB302508), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (AB302508), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFIHC-FrIHC-PWBFlow Cyt (Intra)
Human
Not recommended
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

-

Species

Human

Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info
1/500
Notes

-

Species

Mouse

Dilution info
1/500
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Mouse

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Human

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Target data

Function

Sorting receptor that directs several proteins to their correct location within the cell (Probable). Along with AP-1 complex, involved Golgi apparatus - endosome sorting (PubMed:17646382). Sorting receptor for APP, regulating its intracellular trafficking and processing into amyloidogenic-beta peptides. Retains APP in the trans-Golgi network, hence preventing its transit through late endosomes where amyloid beta peptides Abeta40 and Abeta42 are generated (PubMed:16174740, PubMed:16407538, PubMed:17855360, PubMed:24523320). May also sort newly produced amyloid-beta peptides to lysosomes for catabolism (PubMed:24523320). Does not affect APP trafficking from the endoplasmic reticulum to Golgi compartments (PubMed:17855360). Sorting receptor for the BDNF receptor NTRK2/TRKB that facilitates NTRK2 trafficking between synaptic plasma membranes, postsynaptic densities and cell soma, hence positively regulates BDNF signaling by controlling the intracellular location of its receptor (PubMed:23977241). Sorting receptor for GDNF that promotes GDNF regulated, but not constitutive secretion (PubMed:21994944). Sorting receptor for the GDNF-GFRA1 complex, directing it from the cell surface to endosomes. GDNF is then targeted to lysosomes and degraded, while its receptor GFRA1 recycles back to the cell membrane, resulting in a GDNF clearance pathway. The SORL1-GFRA1 complex further targets RET for endocytosis, but not for degradation, affecting GDNF-induced neurotrophic activities (PubMed:23333276). Sorting receptor for ERBB2/HER2. Regulates ERBB2 subcellular distribution by promoting its recycling after internalization from endosomes back to the plasma membrane, hence stimulating phosphoinositide 3-kinase (PI3K)-dependent ERBB2 signaling. In ERBB2-dependent cancer cells, promotes cell proliferation (PubMed:31138794). Sorting receptor for lipoprotein lipase LPL. Promotes LPL localization to endosomes and later to the lysosomes, leading to degradation of newly synthesized LPL (PubMed:21385844). Potential sorting receptor for APOA5, inducing APOA5 internalization to early endosomes, then to late endosomes, wherefrom a portion is sent to lysosomes and degradation, another portion is sorted to the trans-Golgi network (PubMed:18603531). Sorting receptor for the insulin receptor INSR. Promotes recycling of internalized INSR via the Golgi apparatus back to the cell surface, thereby preventing lysosomal INSR catabolism, increasing INSR cell surface expression and strengthening insulin signal reception in adipose tissue. Does not affect INSR internalization (PubMed:27322061). Plays a role in renal ion homeostasis, controlling the phospho-regulation of SLC12A1/NKCC2 by STK39/SPAK kinase and PPP3CB/calcineurin A beta phosphatase, possibly through intracellular sorting of STK39 and PPP3CB (By similarity). Stimulates, via the N-terminal ectodomain, the proliferation and migration of smooth muscle cells, possibly by increasing cell surface expression of the urokinase receptor uPAR/PLAUR. This may promote extracellular matrix proteolysis and hence facilitate cell migration (PubMed:14764453). By acting on the migration of intimal smooth muscle cells, may accelerate intimal thickening following vascular injury (PubMed:14764453). Promotes adhesion of monocytes (PubMed:23486467). Stimulates proliferation and migration of monocytes/macrophages (By similarity). Through its action on intimal smooth muscle cells and macrophages, may accelerate intimal thickening and macrophage foam cell formation in the process of atherosclerosis (By similarity). Regulates hypoxia-enhanced adhesion of hematopoietic stem and progenitor cells to the bone marrow stromal cells via a PLAUR-mediated pathway. This function is mediated by the N-terminal ectodomain (PubMed:23486467). Metabolic regulator, which functions to maintain the adequate balance between lipid storage and oxidation in response to changing environmental conditions, such as temperature and diet. The N-terminal ectodomain negatively regulates adipose tissue energy expenditure, acting through the inhibition the BMP/Smad pathway (By similarity). May regulate signaling by the heterodimeric neurotrophic cytokine CLCF1-CRLF1 bound to the CNTFR receptor by promoting the endocytosis of the tripartite complex CLCF1-CRLF1-CNTFR and lysosomal degradation (PubMed:26858303). May regulate IL6 signaling, decreasing cis signaling, possibly by interfering with IL6-binding to membrane-bound IL6R, while up-regulating trans signaling via soluble IL6R (PubMed:28265003).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SorLA/SORL1 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23262-4

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SorLA also known as SORL1 or sortilin-related receptor 1 plays a mechanical role in intracellular transport processes. This multi-domain protein with a molecular weight of approximately 250 kDa assists in the trafficking of proteins between the Golgi apparatus endosomes and the cell surface. SorLA expresses most abundantly in neurons but also shows presence in other tissues like the heart and kidney. Its functional expression in these areas indicates a broad role in cellular logistics particularly in neurons where it impacts synaptic functioning.

Biological function summary

SorLA acts as a sorting receptor and contributes significantly to the regulation of protein sorting and signaling pathways within the cell. SorLA interacts with various ligands and forms complexes including lipid transport proteins and neuropeptides. The protein also plays a role in the sorting of the amyloid precursor protein (APP) assisting in the regulation of its processing and reducing the production of amyloid-beta a component significant in neurodegenerative processes.

Pathways

SorLA participates actively in the cellular pathways associated with protein trafficking and processing. It is integral to pathways involving endocytosis and the regulation of APP processing. SorLA interacts with important proteins such as APP and the low-density lipoprotein receptor-related protein 1 (LRP1). These interactions exemplify SorLA’s critical involvement in the cellular maintenance of protein homeostasis and in mitigating the production of potentially pathogenic peptides therefore interfacing with wider neurobiological functions.

Associated diseases and disorders

SorLA’s role connects with neurodegenerative conditions with a notable focus on Alzheimer’s disease. The protein's interaction with APP processing places it in a central position in the pathology of Alzheimer’s disease as dysregulation can lead to increased amyloid-beta production and plaque formation. Additionally SorLA exhibits relevance in some cardiovascular diseases through its modulation of lipoprotein metabolism linking it with the functioning of proteins like apolipoproteins which when disrupted can contribute to the progression of atherosclerosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling SorLA/SORL1 with ab302508 at 1/500 (1.032 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat skeletal muscle (PMID: 9157966) is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh) tissue labeling SorLA/SORL1 with ab302508 at 1/500 (1.032 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat lung (PMID: 9157966) is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SorLA/SORL1 with ab302508 at 1/500 (1.032 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling SorLA/SORL1 with ab302508 at 1/500 (1.032 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse skeletal muscle (PMID: 9157966) is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling SorLA/SORL1 with ab302508 at 1/500 (1.032 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse lung (PMID: 9157966) is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SorLA/SORL1 with ab302508 at 1/500 (1.032 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling SorLA/SORL1 with ab302508 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neural/glia cell.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.Negative control: NIH/3T3 (PMID: 11082041) is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (ab302508)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labelling SorLA/SORL1 with ab302508 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in SK-BR-3 cell lineNegative control: MDA-MB-231 (PMID: 31138794) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com