Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Mouse Monoclonal Sortilin/NT3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Sortilin aa 600-750.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (AB243043), expandable thumbnail

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human Sortilin aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q99523

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000 - 1/10000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000 - 1/10000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 2-10 µg/mL	Notes Fixation/Permeabilization: PFA/Triton X-100

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

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Target data

See full target information Sortilin

Function

Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed:16787399). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

Alternative names

Sortilin, 100 kDa NT receptor, Glycoprotein 95, Neurotensin receptor 3, Gp95, NT3, NTR3, SORT1

Recommended products

Mouse Monoclonal Sortilin/NT3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Sortilin aa 600-750.

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human Sortilin aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q99523
Clone number: CL6526
Purification technique: Affinity purification Protein A
Concentration
Purification notes: Purified from TCS.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sortilin also known as NT3 receptor is a type I membrane protein with an approximate molecular mass of 100 kDa. It is expressed in various tissues especially the central nervous system and adipose tissue. Sortilin belongs to the Vps10p domain receptor family and functions in intracellular protein sorting. Its primary role involves the transport and signaling of neurotrophins particularly NT-3 protein (neurotrophin-3) which is vital for neuronal growth and survival.

Biological function summary

NT-3 protein influences the development and differentiation of the nervous system. Sortilin serves as a receptor for NT-3 facilitating the interaction with the TrkC receptor on neuronal cells. Sortilin forms a complex with NT-3 contributing to critical cellular processes. Being part of this receptor complex Sortilin modulates neuron-related signaling pathways which are essential for neurogenesis and synaptic plasticity.

Pathways

Sortilin and NT-3 play significant roles in the neurotrophin signaling pathway and influence the MAPK signaling cascade. These pathways are pivotal in neuron survival differentiation and growth. NT-3 interacts with other proteins such as TrkC and p75 to propagate these signals. Through its association with TrkC receptor Sortilin mediates critical cellular responses linked to neurotrophin signaling. The interplay between these proteins allows precise regulation of neuronal activities in various physiological states.

Associated diseases and disorders

NT-3 and Sortilin have associations with neurodegenerative conditions such as Alzheimer's disease and metabolic disorders like obesity. Dysregulation of Sortilin can contribute to abnormal NT-3 signaling affecting neuronal homeostasis. The protein also shows links with amyloid precursor protein (APP) in Alzheimer's disease where abnormal interactions may exacerbate disease progression. Understanding the dynamics of Sortilin in these conditions can offer insights into potential therapeutic approaches.

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9 product images

Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraformaldehyde-fixed, Triton X-100 permeabilized RH-30 cells stained for Sortilin/NT3 (Green) using ab243043 at 10 μg/ml in ICC/IF analysis. Microtubules are visulized in red, nucleus in blue.
Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Remaining relative intensity is presented.
Loading control: Anti-GAPDH
All lanes: Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/mL
Lane 1: RH-30 (Human bone marrow metastasis cell line) cells transfected with control siRNA
Lane 2: RH-30 cells transfected with target specific siRNA probe #1
Lane 3: RH-30 cells transfected with target specific siRNA probe #2
Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraffin-embedded mouse hippocampus tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
All lanes: Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/mL
All lanes: SK-MEL-30 cell lysate
Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraffin-embedded mouse cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraffin-embedded human fallopian tube tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraffin-embedded human testis tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraffin-embedded human cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Negative control:
Paraffin-embedded human lymph node tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.