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Rabbit Recombinant Monoclonal Sortilin/NT3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.


Images

Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (AB188586), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR15010] (AB188586), expandable thumbnail
  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (AB188586), expandable thumbnail
  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (AB188586), expandable thumbnail
  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (AB188586), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed:16787399). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

Alternative names

Sortilin, 100 kDa NT receptor, Glycoprotein 95, Neurotensin receptor 3, Gp95, NT3, NTR3, SORT1

Recommended products

Rabbit Recombinant Monoclonal Sortilin/NT3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15010
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sortilin also known as NT3 receptor is a type I membrane protein with an approximate molecular mass of 100 kDa. It is expressed in various tissues especially the central nervous system and adipose tissue. Sortilin belongs to the Vps10p domain receptor family and functions in intracellular protein sorting. Its primary role involves the transport and signaling of neurotrophins particularly NT-3 protein (neurotrophin-3) which is vital for neuronal growth and survival.

Biological function summary

NT-3 protein influences the development and differentiation of the nervous system. Sortilin serves as a receptor for NT-3 facilitating the interaction with the TrkC receptor on neuronal cells. Sortilin forms a complex with NT-3 contributing to critical cellular processes. Being part of this receptor complex Sortilin modulates neuron-related signaling pathways which are essential for neurogenesis and synaptic plasticity.

Pathways

Sortilin and NT-3 play significant roles in the neurotrophin signaling pathway and influence the MAPK signaling cascade. These pathways are pivotal in neuron survival differentiation and growth. NT-3 interacts with other proteins such as TrkC and p75 to propagate these signals. Through its association with TrkC receptor Sortilin mediates critical cellular responses linked to neurotrophin signaling. The interplay between these proteins allows precise regulation of neuronal activities in various physiological states.

Associated diseases and disorders

NT-3 and Sortilin have associations with neurodegenerative conditions such as Alzheimer's disease and metabolic disorders like obesity. Dysregulation of Sortilin can contribute to abnormal NT-3 signaling affecting neuronal homeostasis. The protein also shows links with amyloid precursor protein (APP) in Alzheimer's disease where abnormal interactions may exacerbate disease progression. Understanding the dynamics of Sortilin in these conditions can offer insights into potential therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586), expandable thumbnail

    Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)

    Lanes 1- 2: Merged signal (red and green). Green - ab188586 observed at 100 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab188586 was shown to react with Sortilin/NT3 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human SORT1 (Sortilin/NT3) knockout HeLa cell line ab264772 (knockout cell lysate Human SORT1 (Sortilin/NT3) knockout HeLa cell lysate ab257696) was used. Wild-type HeLa and SORT1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188586 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human SORT1 (Sortilin/NT3) knockout HeLa cell lysate (Human SORT1 (Sortilin/NT3) knockout HeLa cell lysate ab257696) at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Lanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 92 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sortilin/NT3 with ab188586 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586), expandable thumbnail

    Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Sortilin/NT3 knockout HAP1 cell lysate (20 μg)
    Lane 3: SW480 cell lysate (20 μg)
    Lane 4: HeLa cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab188586 observed at 92 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.


    ab188586 was shown to react with Sortilin/NT3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Sortilin/NT3 knockout samples were examined. Wild-type and Sortilin/NT3 knockout samples were subjected to SDS-PAGE. ab188586 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)

    Predicted band size: 92 kDa

  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586), expandable thumbnail

    Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586) at 1/1000 dilution

    Lane 1: SH-SY5Y whole cell lysate at 15 µg

    Lane 2: Human hippocampus tissue lysate at 15 µg

    Lane 3: Human fetal brain tissue lysate at 15 µg

    Lane 4: Mouse brain tissue lysate at 15 µg

    Lane 5: 3T3-L1 whole cell lysate at 15 µg

    Lane 6: Rat brain tissue lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 92 kDa

    Observed band size: 92 kDa

    Exposure time: 30s

  • Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586), expandable thumbnail

    Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)

    All lanes: Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586) at 1/2000 dilution

    Lane 1: SW480 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: SH-SY5Y cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 92 kDa

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Product protocols

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