Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)

Knockout Tested Rabbit Recombinant Monoclonal SOS1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt	WB	ICC/IF
Human	Tested	Not recommended	Not recommended	Tested	Tested
Mouse	Predicted	Not recommended	Not recommended	Predicted	Predicted
Rat	Predicted	Not recommended	Not recommended	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Target data

See full target information SOS1

Function

Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).

Alternative names

Son of sevenless homolog 1, SOS-1, SOS1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal SOS1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR7480
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab248908 is the carrier-free version of Anti-SOS1 antibody [EPR7480] ab140621.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
False colour image of Western blot: Anti-SOS1 antibody [EPR7480] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-SOS1 antibody [EPR7480] ab140621 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line Human SOS1 knockout A-431 cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Twee^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 Â°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: SOS1 knockout A431 cell lysate at 20 µg
Performed under reducing conditions.
Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SOS1 antibody [EPR7480] (Anti-SOS1 antibody [EPR7480] ab140621) at 1/1000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: THP1 cell lysate at 10 µg
Predicted band size: 152 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with Anti-SOS1 antibody [EPR7480] ab140621 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Raji cells labelling SOS1 with Anti-SOS1 antibody [EPR7480] ab140621 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using Anti-SOS1 antibody [EPR7480] ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using Anti-SOS1 antibody [EPR7480] ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using Anti-SOS1 antibody [EPR7480] ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Glioma tissue using Anti-SOS1 antibody [EPR7480] ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using Anti-SOS1 antibody [EPR7480] ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
This data was developed using Anti-SOS1 antibody [EPR7480] ab140621, the same antibody clone in a different buffer formulation.

Anti-SOS1 antibody [EPR7480] (Anti-SOS1 antibody [EPR7480] ab140621) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-SOS1 antibody [EPR7480] ab140621 was shown to bind specifically to SOS1. A band was observed at 165 kDa in wild-type A549 cell lysates with no signal observed at this size in SOS1 knockout cell line. To generate this image, wild-type and SOS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-SOS1 antibody [EPR7480] (Anti-SOS1 antibody [EPR7480] ab140621) at 1/1000 dilution
Lanes 1 - 3: Western blot at 20 µg
Lane 2: Western blot - Human SOS1 knockout A549 cell line (Human SOS1 knockout A549 cell line ab286377)
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 165 kDa