Anti-SOS1 antibody [EPR7480] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation. Anti-SOS1 antibody [EPR7480] (ab140621) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 165 kDa in wild-type A549 cell lysates with no signal observed at this size in SOS1 knockout cell line. To generate this image, wild-type and SOS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SOS1 antibody [EPR7480] (<a href='/en-us/products/primary-antibodies/sos1-antibody-epr7480-ab140621'>ab140621</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot at 20 µg

Lane 2:

Western blot - Human SOS1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-sos1-knockout-a549-cell-line-ab286377'>ab286377</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 152 kDa

Observed band size: 165 kDa

false

• WB

Unknown

Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

This data was developed using ab140621, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SOS1 antibody [EPR7480] (<a href='/en-us/products/primary-antibodies/sos1-antibody-epr7480-ab140621'>ab140621</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

THP1 cell lysate at 10 µg

Predicted band size: 152 kDa

false

• WB

Lab

Western blot - Anti-SOS1 antibody [EPR7480] - BSA and Azide free (AB248908)

False colour image of Western blot : Anti-SOS1 antibody [EPR7480] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Twee^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

SOS1 knockout A431 cell lysate at 20 µg

false